Abstract:
:Enterohemorrhagic Escherichia coli O157:H7 has a natural reservoir in the intestinal tracts of cattle. Colonization is asymptomatic and transient, but it is not clear if protective immunity is induced. This study demonstrates that prior colonization induces humoral immune responses to bacterial antigens and reduces bacterial shedding after experimental challenge with the homologous strain.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Naylor SW,Flockhart A,Nart P,Smith DG,Huntley J,Gally DL,Low JCdoi
10.1128/AEM.02670-06subject
Has Abstractpub_date
2007-06-01 00:00:00pages
3765-7issue
11eissn
0099-2240issn
1098-5336pii
AEM.02670-06journal_volume
73pub_type
杂志文章abstract::This study concerns the exploitation of the lytic enzyme enterolysin A (EntL), produced by Enterococcus faecalis strain DPC5280, to elicit the controlled autolysis of starter lactococci. EntL, a cell wall metalloendopeptidase secreted by some E. faecalis strains, can kill a wide range of gram-positive bacteria, includ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.3.1744-1748.2004
更新日期:2004-03-01 00:00:00
abstract::We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.2.504-513.2001
更新日期:2001-02-01 00:00:00
abstract::The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4440-4447.2001
更新日期:2001-10-01 00:00:00
abstract::The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2818-2825.1990
更新日期:1990-09-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00252-16
更新日期:2016-05-02 00:00:00
abstract::Artificially designed gelatins comprising tandemly repeated 30-amino-acid peptide units derived from human alphaI collagen were successfully produced with a Bacillus brevis system. The DNA encoding the peptide unit was synthesized by taking into consideration the codon usage of the host cells, but no clones having a t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.1.304-309.2000
更新日期:2000-01-01 00:00:00
abstract::The gene cluster that codes for feedback-resistant aspartate kinase (lysCalpha and lysCbeta) and aspartate semialdehyde dehydrogenase (asd) was cloned from a mutant strain of Corynebacterium glutamicum. Its functional analysis by subcloning, enzyme assays, and type of aspartate kinase regulation enabled the isolation ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.6.1746-1752.1991
更新日期:1991-06-01 00:00:00
abstract::Disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) gave Aspergillus parasiticus transformants that accumulated versicolorin A. This gene is predicted to encode the Baeyer-Villiger oxidase necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.12.8963-8965.2005
更新日期:2005-12-01 00:00:00
abstract::Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.5.1207-1208.1977
更新日期:1977-05-01 00:00:00
abstract::Sodium gradients in cyanobacteria play an important role in energy storage under photoautotrophic conditions but have not been well studied during autofermentative metabolism under the dark, anoxic conditions widely used to produce precursors to fuels. Here we demonstrate significant stress-induced acceleration of aut...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00612-11
更新日期:2011-10-01 00:00:00
abstract::Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.3.597-603.1982
更新日期:1982-09-01 00:00:00
abstract::Phytophthora ramorum is a recently described pathogen causing oak mortality (sudden oak death) in forests in coastal areas of California and southern Oregon and dieback and leaf blight in a range of tree, shrub, and herbaceous species in the United States and Europe. Due to the threat posed by this organism, stringent...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.11.6702-6710.2005
更新日期:2005-11-01 00:00:00
abstract::Compost amendment and inoculations with specific microorganisms are fundamentally different soil treatment methods, commonly used in agriculture for the improvement of plant growth and health. Although distinct, both methods affect the rhizosphere and the plant roots. In the present study we used a 16S rRNA gene appro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00736-09
更新日期:2009-10-01 00:00:00
abstract::In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00026-12
更新日期:2012-07-01 00:00:00
abstract::Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.7.4035-4039.2004
更新日期:2004-07-01 00:00:00
abstract::Vibrio parahaemolyticus is indigenous to coastal environments and a frequent cause of seafood-borne gastroenteritis in the United States, primarily due to raw-oyster consumption. Previous seasonal-cycle studies of V. parahaemolyticus have identified water temperature as the strongest environmental predictor. Salinity ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01700-07
更新日期:2007-12-01 00:00:00
abstract::A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.8.4888-4892.2005
更新日期:2005-08-01 00:00:00
abstract::[This corrects the article on p. 2161 in vol. 59.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Salinity effects on microbial community structure and on potential rates of arsenate reduction, arsenite oxidation, sulfate reduction, denitrification, and methanogenesis were examined in sediment slurries from two California soda lakes. We conducted experiments with Mono Lake and Searles Lake sediments over a wide ra...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00771-07
更新日期:2007-08-01 00:00:00
abstract::Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01536-18
更新日期:2018-10-30 00:00:00
abstract::The understanding of the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-C]linoleic acid or [5-C]sodium mevalonate revealed that both fungal strains...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.9.2945-2950.1993
更新日期:1993-09-01 00:00:00
abstract::Mold-damaged building materials may contain biologically active agents, such as (1-->3)-beta-D-glucan, allergens, and mycotoxins, which have been associated with adverse health effects. The release of these components from contaminated surfaces into the air is not well understood. The purpose of this study was to char...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01791-07
更新日期:2008-02-01 00:00:00
abstract::A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.4.771-776.1978
更新日期:1978-04-01 00:00:00
abstract::A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.7.1761-1765.1988
更新日期:1988-07-01 00:00:00
abstract::Recent declines in bumble bee populations are of great concern and have prompted critical evaluations of the role of pathogen introductions and host resistance in bee health. One factor that may influence host resilience when facing infection is the gut microbiota. Previous experiments with Bombus terrestris, a Europe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02335-17
更新日期:2018-03-19 00:00:00
abstract::Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.2.416-421.1987
更新日期:1987-02-01 00:00:00
abstract::Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01693-14
更新日期:2014-10-01 00:00:00
abstract::A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures....
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.3.1150-1152.1995
更新日期:1995-03-01 00:00:00
abstract::A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03793-14
更新日期:2015-04-01 00:00:00
abstract::Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. T...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.4.3016-3020.2006
更新日期:2006-04-01 00:00:00