Isolation, sequence, and characterization of the Cercospora nicotianae phytoene dehydrogenase gene.

Abstract:

:We have cloned and sequenced the Cercospora nicotianae gene for the carotenoid biosynthetic enzyme phytoene dehydrogenase. Analysis of the derived amino acid sequence revealed it has greater than 50% identity with its counterpart in Neurospora crassa and approximately 30% identity with prokaryotic phytoene dehydrogenases and is related, but more distantly, to phytoene dehydrogenases from plants and cyanobacteria. Our analysis confirms that phytoene dehydrogenase proteins fall into two groups: those from plants and cyanobacteria and those from eukaryotic and noncyanobacter prokaryotic microbes. Southern analysis indicated that the C. nicotianae phytoene dehydrogenase gene is present in a single copy. Extraction of beta-carotene, the sole carotenoid accumulated by C. nicotianae, showed that both light- and dark-grown cultures synthesize carotenoids, but higher levels accumulate in the light. Northern (RNA) analysis of poly(A)+ RNA, however, showed no differential accumulation of phytoene dehydrogenase mRNA between light- and dark-grown fungal cultures.

journal_name

Appl Environ Microbiol

authors

Ehrenshaft M,Daub ME

doi

10.1128/AEM.60.8.2766-2771.1994

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

2766-71

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

60

pub_type

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