Inhibition of beta-galactosidase biosynthesis in Escherichia coli by tetracycline residues in milk.

Abstract:

:Low levels of tetracyclines found as residues in milk inhibited the biosynthesis of beta-galactosidase in Escherichia coli. To produce the same effect, other antibacterials had to occur in concentrations that were more than 10-fold higher. This relative selectivity was exploited for the development of a screening test for tetracyclines in milk based on a chemiluminometric assay of beta-galactosidase. The method was validated with spiked samples of raw milk and applied to field samples contaminated with tetracyclines.

journal_name

Appl Environ Microbiol

authors

D'Haese E,Nelis HJ,Reybroeck W

doi

10.1128/AEM.63.10.4116-4119.1997

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

4116-9

issue

10

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

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