Interaction of transcriptional repressor ArgR with transcriptional regulator FarR at the argB promoter region in Corynebacterium glutamicum.

abstract：:A compound required for the luxuriant growth of Lactobacillus sanfrancisco in laboratory media was isolated from a freshly prepared yeast extract, purified, and characterized. The stimulant was identified as a small peptide with a molecular weight of approximately 1,065, containing aspartic acid, cysteine, glutamic ac...

journal_title：Applied and environmental microbiology

authors： Berg RW,Sandine WE,Anderson AW

更新日期：1981-11-01 00:00:00

abstract：:The haloalkane dehalogenase enzyme DmmA was identified by marine metagenomic screening. Determination of its crystal structure revealed an unusually large active site compared to those of previously characterized haloalkane dehalogenases. Here we present a biochemical characterization of this interesting enzyme with e...

journal_title：Applied and environmental microbiology

authors： Buryska T,Babkova P,Vavra O,Damborsky J,Prokop Z

更新日期：2018-01-02 00:00:00

abstract：:The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to c...

journal_title：Applied and environmental microbiology

authors： Clarkson SM,Giannone RJ,Kridelbaugh DM,Elkins JG,Guss AM,Michener JK

更新日期：2017-08-31 00:00:00

abstract：:A study of six child care centers was conducted to assess the microbiological quality of three food contact surfaces (one food serving surface and two food preparation surfaces) and one non-food contact surface (diaper changing surface) to determine the effectiveness of cleaning and sanitization procedures within the ...

journal_title：Applied and environmental microbiology

authors： Cosby CM,Costello CA,Morris WC,Haughton B,Devereaux MJ,Harte F,Davidson PM

更新日期：2008-11-01 00:00:00

abstract：:Three expedition huts in the Ross Sea region of Antarctica, built between 1901 and 1911 by Robert F. Scott and Ernest Shackleton, sheltered and stored the supplies for up to 48 men for 3 years during their explorations and scientific investigation in the South Pole region. The huts, built with wood taken to Antarctica...

journal_title：Applied and environmental microbiology

authors： Blanchette RA,Held BW,Jurgens JA,McNew DL,Harrington TC,Duncan SM,Farrell RL

更新日期：2004-03-01 00:00:00

abstract：:The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitr...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Anda M,Inaba S,Eda S,Sato S,Sasaki K,Tabata S,Mitsui H,Sato T,Shinano T,Minamisawa K

更新日期：2011-03-01 00:00:00

abstract：:The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Borneman J,Triplett EW

更新日期：1998-12-01 00:00:00

abstract：:The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

journal_title：Applied and environmental microbiology

authors： Lakshmidevi G,Davidson BE,Hillier AJ

更新日期：1988-04-01 00:00:00

abstract：:Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...

journal_title：Applied and environmental microbiology

authors： Fuentes FA,Biamon EJ,Hazen TC

更新日期：1983-12-01 00:00:00

abstract：:We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

journal_title：Applied and environmental microbiology

authors： Nakayama A,Yano Y,Yoshida K

更新日期：1994-11-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:Compared to freshwater and the open ocean, less is known about bacterioplankton community structure and spatiotemporal dynamics in estuaries, particularly those with long residence times. The Chesapeake Bay is the largest estuary in the United States, but despite its ecological and economic significance, little is kno...

journal_title：Applied and environmental microbiology

authors： Kan J,Suzuki MT,Wang K,Evans SE,Chen F

更新日期：2007-11-01 00:00:00

abstract：:Zoothamnium niveum is a giant, colonial marine ciliate from sulfide-rich habitats obligatorily covered with chemoautotrophic, sulfide-oxidizing bacteria which appear as coccoid rods and rods with a series of intermediate shapes. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization showed ...

journal_title：Applied and environmental microbiology

authors： Rinke C,Schmitz-Esser S,Stoecker K,Nussbaumer AD,Molnár DA,Vanura K,Wagner M,Horn M,Ott JA,Bright M

更新日期：2006-03-01 00:00:00

abstract：:rRNA internal transcribed spacer phylogeny showed that Chesapeake Bay is populated with diverse Synechococcus strains, including members of the poorly studied marine cluster B. Marine cluster B prevailed in the upper bay, while marine cluster A was common in the lower bay. Interestingly, marine cluster B Synechococcus...

journal_title：Applied and environmental microbiology

authors： Chen F,Wang K,Kan J,Suzuki MT,Wommack KE

更新日期：2006-03-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal ...

journal_title：Applied and environmental microbiology

authors： Hamby KA,Hernández A,Boundy-Mills K,Zalom FG

更新日期：2012-07-01 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：:The incidence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection has been increasing; however, the sources of infection remain unclear. Therefore, we investigated the involvement of meat as a possible mediator of CA-MRSA infection. We examined the distribution of MRSA strains in comm...

journal_title：Applied and environmental microbiology

authors： Ogata K,Narimatsu H,Suzuki M,Higuchi W,Yamamoto T,Taniguchi H

更新日期：2012-04-01 00:00:00

abstract：:A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

journal_title：Applied and environmental microbiology

authors： Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

更新日期：2006-10-01 00:00:00

abstract：:The lactose operon from Lactobacillus casei is regulated by very tight glucose repression and substrate induction mechanisms, which made it a tempting candidate system for the expression of foreign genes or metabolic engineering. An integrative vector was constructed, allowing stable gene insertion in the chromosomal ...

journal_title：Applied and environmental microbiology

authors： Gosalbes MJ,Esteban CD,Galán JL,Pérez-Martínez G

更新日期：2000-11-01 00:00:00

abstract：:Molecular tools that can provide an estimate of the in situ growth rate of Geobacter species could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole-genome microarray analyses of a subsurface isolate of Geobacter uraniireducens, grown under a variety of conditions, identified...

journal_title：Applied and environmental microbiology

authors： Holmes DE,Giloteaux L,Barlett M,Chavan MA,Smith JA,Williams KH,Wilkins M,Long P,Lovley DR

更新日期：2013-03-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:The influence of antimicrobial agents on the development of antimicrobial resistance (AMR) in Campylobacter isolates recovered from 300 beef cattle maintained in an experimental feedlot was monitored over a 315-day period (11 sample times). Groups of calves were assigned to one of the following antimicrobial treatment...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McAllister TA,Busz HW,Yanke LJ,Morck DW,Olson ME,Read RR

更新日期：2005-07-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:Three different strains of bacteria isolated from spoiled, uncooked chicken were grown in pure culture on Trypticase soy agar supplemented with yeast extract. The volatile organic compounds produced by each culture were concentrated on a porous polymer precolumn and analyzed by high-resolution gas chromatographic mass...

journal_title：Applied and environmental microbiology

authors： Lee ML,Smith DL,Freeman LR

更新日期：1979-01-01 00:00:00

abstract：:Growth on 3-chlorobenzoate was found to induce the enzymes of the protocatechuate meta ring fission pathway in Alcaligenes sp. strain BR60. The chlorobenzoate catabolic genes, designated cba, were localized to a 3.7-kb NotI-EcoRI fragment within the nonrepeated region of the composite transposon Tn5271. The cba genes ...

journal_title：Applied and environmental microbiology

authors： Nakatsu CH,Wyndham RC

更新日期：1993-11-01 00:00:00

abstract：:We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...

journal_title：Applied and environmental microbiology

authors： Sharma NK,Rees CE,Dodd CE

更新日期：2000-04-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:The phylogenetic diversity of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, E.C. 4.1.1.39) large-subunit genes of deep-sea microorganisms was analyzed. Bulk genomic DNA was isolated from seven samples, including samples from the Mid-Atlantic Ridge and various deep-sea habitats around Japan. The kinds o...

journal_title：Applied and environmental microbiology

authors： Elsaied H,Naganuma T

更新日期：2001-04-01 00:00:00