Oxygen demand by lipid-accumulating yeasts in continuous culture.

abstract：:The dynamic control of gene expression is important for adjusting fluxes in order to obtain desired products and achieve appropriate cell growth, particularly when the synthesis of a desired product drains metabolites required for cell growth. For dynamic gene expression, a promoter responsive to a particular environm...

journal_title：Applied and environmental microbiology

authors： Yin X,Shin HD,Li J,Du G,Liu L,Chen J

更新日期：2017-03-02 00:00:00

abstract：:Eighteen strains of Agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. Of six agrocin-producing strains, one (D286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type Ti plasmids. Sensitivity to agrocin D286 was found to map in...

journal_title：Applied and environmental microbiology

authors： Hendson M,Askjaer L,Thomson JA,van Montagu M

更新日期：1983-05-01 00:00:00

abstract：:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detect...

journal_title：Applied and environmental microbiology

authors： Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg H

更新日期：2015-12-11 00:00:00

abstract：:A model conditional-suicide system to control genetically engineered microorganisms able to degrade substituted benzoates is reported. The system is based on two elements. One element consists of a fusion between the promoter of the Pseudomonas putida TOL plasmid-encoded meta-cleavage pathway operon (P(m)) and the lac...

journal_title：Applied and environmental microbiology

authors： Contreras A,Molin S,Ramos JL

更新日期：1991-05-01 00:00:00

abstract：:The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitr...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Anda M,Inaba S,Eda S,Sato S,Sasaki K,Tabata S,Mitsui H,Sato T,Shinano T,Minamisawa K

更新日期：2011-03-01 00:00:00

abstract：:Rhodobacter capsulatus E1F1, a phototrophic purple nonsulfur bacterium capable of photoassimilating nitrate or nitrite, grew phototrophically in the presence of mono- and dinitrophenols with acetate as a carbon source, the highest growth levels being obtained under microaerobic conditions. Utilization of 2,4-dinitroph...

journal_title：Applied and environmental microbiology

authors： Blasco R,Castillo F

更新日期：1992-02-01 00:00:00

abstract：:The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h(-1) for 30 min and 25 mm h(-1) for 180 min were applied to soil plots with artificial fecal pats seeded with app...

journal_title：Applied and environmental microbiology

authors： Davies CM,Ferguson CM,Kaucner C,Krogh M,Altavilla N,Deere DA,Ashbolt NJ

更新日期：2004-02-01 00:00:00

abstract：:We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium oxidation (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized Fe(2+) and reduced NO3(-) to nitrogen gas at rates of 3.7 ± 0.2 and 1.3 ± 0.1 (mean ± standard deviation [SD]) nmol mg protein(-1) min(-...

journal_title：Applied and environmental microbiology

authors： Oshiki M,Ishii S,Yoshida K,Fujii N,Ishiguro M,Satoh H,Okabe S

更新日期：2013-07-01 00:00:00

abstract：:Efficient enrichment of staphylococcal cells displaying specific heterologous affinity ligands on their cell surfaces was demonstrated by using fluorescence-activated cell sorting. Using bacterial surface display of peptide or protein libraries for the purpose of combinatorial protein engineering has previously been i...

journal_title：Applied and environmental microbiology

authors： Wernérus H,Samuelson P,Ståhl S

更新日期：2003-09-01 00:00:00

abstract：:Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. Percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis A virus, 77 for human...

journal_title：Applied and environmental microbiology

authors： Lewis GD,Metcalf TG

更新日期：1988-08-01 00:00:00

abstract：:This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport l-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport syste...

journal_title：Applied and environmental microbiology

authors： Eighmy TT,Bishop PL

更新日期：1985-07-01 00:00:00

abstract：:The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

journal_title：Applied and environmental microbiology

authors： Alvarez AJ,Buttner MP,Stetzenbach LD

更新日期：1995-10-01 00:00:00

abstract：:Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not ind...

journal_title：Applied and environmental microbiology

authors： Abbaszadegan M,Huber MS,Gerba CP,Pepper IL

更新日期：1997-01-01 00:00:00

abstract：:The bacterial pathogen Vibrio vulnificus is found naturally in brackish coastal waters but can be greatly concentrated by filter-feeding organisms such as shellfish. Numerous experiments in which exogenous V. vulnificus cells are added to oysters in an attempt to measure uptake and depuration have been performed. In n...

journal_title：Applied and environmental microbiology

authors： Froelich B,Oliver J

更新日期：2013-09-01 00:00:00

abstract：:Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

journal_title：Applied and environmental microbiology

authors： Picardeau M

更新日期：2008-01-01 00:00:00

abstract：:Typing of F-specific RNA (FRNA) coliphages has been proposed as a useful method for distinguishing human from animal fecal contamination in environmental samples. Group II and III FRNA coliphages are generally associated with human wastes, but several exceptions have been noted. In the present study, we have genotyped...

journal_title：Applied and environmental microbiology

authors： Stewart JR,Vinjé J,Oudejans SJ,Scott GI,Sobsey MD

更新日期：2006-02-01 00:00:00

abstract：:Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

journal_title：Applied and environmental microbiology

authors： Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

更新日期：2010-12-01 00:00:00

abstract：:Pierce's disease is a devastating lethal disease of Vitus vinifera grapevines caused by the bacterium Xylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the...

journal_title：Applied and environmental microbiology

authors： Azizi A,Arora A,Markiv A,Lampe DJ,Miller TA,Kang AS

更新日期：2012-04-01 00:00:00

abstract：:The survival of microorganisms can be predicted through the use of active biological containment systems. We have constructed contained Pseudomonas putida strains that degrade alkylbenzoates. The modified strain carries a fusion of the Plac promoter to the gef gene, which encodes a killing protein. Expression from Pla...

journal_title：Applied and environmental microbiology

authors： Ronchel MC,Ramos C,Jensen LB,Molin S,Ramos JL

更新日期：1995-08-01 00:00:00

abstract：:Vibrio cholerae non-O1/non-O139 strains have caused several cases of ear, wound, and blood infections, including one lethal case of septicemia in Austria, during recent years. All of these cases had a history of local recreational activities in the large eastern Austrian lake Neusiedler See. Thus, a monitoring program...

journal_title：Applied and environmental microbiology

authors： Kirschner AK,Schlesinger J,Farnleitner AH,Hornek R,Süss B,Golda B,Herzig A,Reitner B

更新日期：2008-04-01 00:00:00

abstract：:We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the c...

journal_title：Applied and environmental microbiology

authors： Zwirglmaier K,Ludwig W,Schleifer KH

更新日期：2004-01-01 00:00:00

abstract：:Previously isolated dissimilatory perchlorate-reducing bacteria (DPRB) have been primarily affiliated with the Betaproteobacteria. Enrichments from the cathodic chamber of a bioelectrical reactor (BER) inoculated from creek water in Berkeley, CA, yielded a novel organism most closely related to a previously described ...

journal_title：Applied and environmental microbiology

authors： Thrash JC,Ahmadi S,Torok T,Coates JD

更新日期：2010-07-01 00:00:00

abstract：:The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest ...

journal_title：Applied and environmental microbiology

authors： Frostegård A,Tunlid A,Bååth E

更新日期：1993-11-01 00:00:00

abstract：:Intact core tetraether membrane lipids of marine planktonic Crenarchaeota were quantified in water column-suspended particulate matter obtained from four depth intervals ( approximately 70, 500, 1,000 and 1,500 m) at seven stations in the northwestern Arabian Sea to investigate the distribution of the organisms at var...

journal_title：Applied and environmental microbiology

authors： Sinninghe Damsté JS,Rijpstra WI,Hopmans EC,Prahl FG,Wakeham SG,Schouten S

更新日期：2002-06-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:Two microorganisms which could degrade soybean lees efficiently were isolated and identified as Bacillus circulans and B. stearothermophilus. These two strains secreted thermostable proteases into the medium and could digest soybean lees rapidly and completely at 50 degrees C. Initially, the soybean lees were degraded...

journal_title：Applied and environmental microbiology

authors： Kubo M,Okajima J,Hasumi F

更新日期：1994-01-01 00:00:00

abstract：:The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

journal_title：Applied and environmental microbiology

authors： Prince HN

更新日期：1978-08-01 00:00:00

abstract：:Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...

journal_title：Applied and environmental microbiology

authors： Murinda SE,Roberts RF,Wilson RA

更新日期：1996-09-01 00:00:00

abstract：:To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

journal_title：Applied and environmental microbiology

authors： Richter D,Schlee DB,Allgöwer R,Matuschka FR

更新日期：2004-11-01 00:00:00

abstract：:More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

journal_title：Applied and environmental microbiology

authors： Hahn MW

更新日期：2003-09-01 00:00:00