Quantitative determination of the biodegradable polymer Poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics.

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:The efficiency of a system developed for the recovery of viruses contaminating large quantities of vegetables was investigated in the laboratory and tested in the field. Viruses seeded onto a number of leafy vegetables in the laboratory were eluted with a phosphate-buffered saline solution (pH 9.0). The eluate was cla...

journal_title：Applied and environmental microbiology

authors： Ward BK,Chenoweth CM,Irving LG

更新日期：1982-12-01 00:00:00

abstract：:Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

journal_title：Applied and environmental microbiology

authors： Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

更新日期：2013-05-01 00:00:00

abstract：:Saturation point mutagenesis was carried out at position 479 in the polyhydroxyalkanoate (PHA) synthase from Chromobacterium sp. strain USM2 (PhaC(Cs)) with specificities for short-chain-length (SCL) [(R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyvalerate (3HV)] and medium-chain-length (MCL) [(R)-3-hydroxyhexanoate (3H...

journal_title：Applied and environmental microbiology

authors： Chuah JA,Tomizawa S,Yamada M,Tsuge T,Doi Y,Sudesh K,Numata K

更新日期：2013-06-01 00:00:00

abstract：:Laccase is a copper-containing phenoloxidase, involved in lignin degradation by white rot fungi. The laccase substrate range can be extended to include nonphenolic lignin subunits in the presence of a noncatalytic cooxidant such as 2, 2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), with ABTS being oxidized to t...

journal_title：Applied and environmental microbiology

authors： Collins PJ,Dobson ADW,Field JA

更新日期：1998-06-01 00:00:00

abstract：:In samples of flooded soil containing blue-green algae (cyanobacteria), the presence of rice plants did not influence the nitrogenase activity of the algae. Nitrogenase activity of heterotrophic bacteria was enhanced by the presence of rice plants, but this activity was not affected by changes in plant density. The ra...

journal_title：Applied and environmental microbiology

authors： Habte M,Alexander M

更新日期：1980-09-01 00:00:00

abstract：:The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Oberleiter A,Psenner R

更新日期：1996-12-01 00:00:00

abstract：:Cronobacter species are opportunistic pathogens commonly found in the environment. Among the seven Cronobacter species, Cronobacter sakazakii sequence type 4 (ST-4) is predominantly associated with recorded cases of infantile meningitis. This study reports on a 26-month powdered infant formula (PIF) surveillance progr...

journal_title：Applied and environmental microbiology

authors： Yan Q,Wang J,Gangiredla J,Cao Y,Martins M,Gopinath GR,Stephan R,Lampel K,Tall BD,Fanning S

更新日期：2015-07-01 00:00:00

abstract：:Dibenzothiophene is degraded to 3-hydroxy-2-formyl benzothiophene by various bacteria, including a strain of Pseudomonas putida that also forms dibenzothiophene sulfone via an alternate pathway. By using these end products as substrates, mixed enrichment cultures that could degrade 3-hydroxy-2-formyl benzothiophene an...

journal_title：Applied and environmental microbiology

authors： Mormile MR,Atlas RM

更新日期：1988-12-01 00:00:00

abstract：:Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

journal_title：Applied and environmental microbiology

authors： Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

更新日期：2020-01-07 00:00:00

abstract：:To evaluate the microbial populations involved in the reduction of Fe(III) in an acidic, iron-rich sediment, the anaerobic flow of supplemental carbon and reductant was evaluated in sediment microcosms at the in situ temperature of 12 degrees C. Supplemental glucose and cellobiose stimulated the formation of Fe(II); 4...

journal_title：Applied and environmental microbiology

authors： Küsel K,Dorsch T,Acker G,Stackebrandt E

更新日期：1999-08-01 00:00:00

abstract：:The ability of indigenous Rhizobium leguminosarum and Rhizobium meliloti to use organic nutrients as growth substrates in soil was assessed by indirect bacteriophage analysis. A total of 17 organic compounds, including 9 carbohydrates, 3 organic acids, and 5 amino acids, were tested (1,000 mug g) in three soils with d...

journal_title：Applied and environmental microbiology

authors： Germida JJ

更新日期：1988-01-01 00:00:00

abstract：:The thermophilic homoacetogenic bacterium Moorella sp. strain HUC22-1 ferments glyoxylate to acetate roughly according to the reaction 2 glyoxylate --> acetate + 2 CO(2). A batch culture with glyoxylate and yeast extract yielded 11.7 g per mol of cells per substrate, which was much higher than that obtained with H(2) ...

journal_title：Applied and environmental microbiology

authors： Sakai S,Inokuma K,Nakashimada Y,Nishio N

更新日期：2008-03-01 00:00:00

abstract：:Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...

journal_title：Applied and environmental microbiology

authors： Villena I,Aubert D,Gomis P,Ferté H,Inglard JC,Denis-Bisiaux H,Dondon JM,Pisano E,Ortis N,Pinon JM

更新日期：2004-07-01 00:00:00

abstract：:Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soil...

journal_title：Applied and environmental microbiology

authors： Velázquez E,Martínez-Romero E,Rodríguez-Navarro DN,Trujillo ME,Daza A,Mateos PF,Martínez-Molina E,van Berkum P

更新日期：2001-02-01 00:00:00

abstract：:Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limi...

journal_title：Applied and environmental microbiology

authors： Olstorpe M,Schnürer J,Passoth V

更新日期：2012-01-01 00:00:00

abstract：:The genus Exiguobacterium can adapt readily to, and survive in, diverse environments. Our study demonstrated that Exiguobacterium sp. strain S3-2, isolated from marine sediment, is resistant to five antibiotics. The plasmid pMC1 in this strain carries seven putative resistance genes. We functionally characterized thes...

journal_title：Applied and environmental microbiology

authors： Yang J,Wang C,Wu J,Liu L,Zhang G,Feng J

更新日期：2014-02-01 00:00:00

abstract：:The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

journal_title：Applied and environmental microbiology

authors： Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

更新日期：2015-07-01 00:00:00

abstract：:A collection of 13 field isolates of Rhizobium leguminosarum bv. viciae that have the ability to nodulate the roots of current North American cultivars of peas as well as a "primitive" cultivar, Afghanistan, was examined. These isolates originated in diverse geographical regions of the world, which indicates that this...

journal_title：Applied and environmental microbiology

authors： Ma SW,Iyer VN

更新日期：1990-07-01 00:00:00

abstract：:Using scanning electron microscopy, we observed sucker-like structures on amoebae of 13 human isolates of Naegleria fowleri. The number of suckers per amoeba seemed to vary according to the virulence of the strain. We propose the term amoebastome to describe this unique sucker-like structure of N. fowleri. ...

journal_title：Applied and environmental microbiology

authors： John DT,Cole TB Jr,Marciano-Cabral FM

更新日期：1984-01-01 00:00:00

abstract：:A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp ...

journal_title：Applied and environmental microbiology

authors： Sahl JW,Schmidt R,Swanner ED,Mandernack KW,Templeton AS,Kieft TL,Smith RL,Sanford WE,Callaghan RL,Mitton JB,Spear JR

更新日期：2008-01-01 00:00:00

abstract：:Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobact...

journal_title：Applied and environmental microbiology

authors： Francis AJ,Dobbs S,Nine BJ

更新日期：1980-07-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:The LlaDII restriction/modification (R/M) system was found on the naturally occurring 8.9-kb plasmid pHW393 in Lactococcus lactis subsp. cremoris W39. A 2.4-kb PstI-EcoRI fragment inserted into the Escherichia coli-L. lactis shuttle vector pCI3340 conferred to L. lactis LM2301 and L. lactis SMQ86 resistance against re...

journal_title：Applied and environmental microbiology

authors： Madsen A,Josephsen J

更新日期：1998-07-01 00:00:00

abstract：:Antibody production against aflatoxin B1 was compared in three rabbits and one goat. Titers obtained were 20 times higher in the rabbits than in the goat. The goat antiserum appeared to have a higher degree of cross-reactivity for other aflatoxins and related metabolites than did the rabbit antiserum. ...

journal_title：Applied and environmental microbiology

authors： Gaur PK,El-Nakib O,Chu FS

更新日期：1980-09-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:N-acylhomoserine lactones (AHLs) are signaling molecules in many quorum-sensing (QS) systems that regulate interactions between various pathogenic bacteria and their hosts. Quorum quenching by the enzymatic inactivation of AHLs holds great promise in preventing and treating infections, and several such enzymes have be...

journal_title：Applied and environmental microbiology

authors： Mei GY,Yan XX,Turak A,Luo ZQ,Zhang LQ

更新日期：2010-08-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

journal_title：Applied and environmental microbiology

authors： Hawkins AB,Adams MW,Kelly RM

更新日期：2014-04-01 00:00:00

abstract：:This study was initiated to determine if reduced sulfur contained in coal gasifier ash and slag was available for microbial and chemical oxidation because eventual large-quantity landfill disposal of these solid wastes is expected. Continuous application of distilled water to a column containing a high-sulfur-content ...

journal_title：Applied and environmental microbiology

authors： Strayer RF,Davis EC

更新日期：1983-03-01 00:00:00