Reduction of the 2,2'-Azinobis(3-ethylbenzthiazoline-6-sulfonate) cation radical by physiological organic acids in the absence and presence of manganese

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detect...

journal_title：Applied and environmental microbiology

authors： Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg H

更新日期：2015-12-11 00:00:00

abstract：:Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate betwe...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Ast JC

更新日期：2005-02-01 00:00:00

abstract：:The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

journal_title：Applied and environmental microbiology

authors： Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

更新日期：2007-03-01 00:00:00

abstract：:Lactococcin G and enterocin 1071 are two homologous two-peptide bacteriocins. Expression vectors containing the gene encoding the putative lactococcin G immunity protein (lagC) or the gene encoding the enterocin 1071 immunity protein (entI) were constructed and introduced into strains sensitive to one or both of the b...

journal_title：Applied and environmental microbiology

authors： Oppegård C,Emanuelsen L,Thorbek L,Fimland G,Nissen-Meyer J

更新日期：2010-02-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:There are a number of genetic tools available for studying Francisella tularensis, the etiological agent of tularemia; however, there is no effective inducible or repressible gene expression system. Here, we describe inducible and repressible gene expression systems for F. tularensis based on the Tet repressor, TetR. ...

journal_title：Applied and environmental microbiology

authors： LoVullo ED,Miller CN,Pavelka MS Jr,Kawula TH

更新日期：2012-10-01 00:00:00

abstract：:Torula corallina, a strain presently being used for the industrial production of erythritol, has the highest erythritol yield ever reported for an erythritol-producing microorganism. The increased production of erythritol by Torula corallina with trace elements such as Cu(2+) has been thoroughly reported, but the mech...

journal_title：Applied and environmental microbiology

authors： Lee JK,Koo BS,Kim SY

更新日期：2002-09-01 00:00:00

abstract：:The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

journal_title：Applied and environmental microbiology

authors： Hai T,Frey KM,Steinbüchel A

更新日期：2006-12-01 00:00:00

abstract：:The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...

journal_title：Applied and environmental microbiology

authors： Widmer G,Lee Y

更新日期：2010-10-01 00:00:00

abstract：:Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

journal_title：Applied and environmental microbiology

authors： Murphy SK,Oliver JD

更新日期：1992-09-01 00:00:00

abstract：:The nucleotide sequence of a new plasmid pCNB1 from Comamonas sp. strain CNB-1 that degrades 4-chloronitrobenzene (4CNB) was determined. pCNB1 belongs to the IncP-1beta group and is 91,181 bp in length. A total of 95 open reading frames appear to be involved in (i) the replication, maintenance, and transfer of pCNB1; ...

journal_title：Applied and environmental microbiology

authors： Ma YF,Wu JF,Wang SY,Jiang CY,Zhang Y,Qi SW,Liu L,Zhao GP,Liu SJ

更新日期：2007-07-01 00:00:00

abstract：:The bacterial communities associated with the development of necroses in injured agria cactus tissue were examined in the field by using both human-induced injuries and cactus tissue inoculated with cactophilic bacteria. Whole-cell bacterial fatty acids were used to determine when and where each of 23 detected species...

journal_title：Applied and environmental microbiology

authors： Foster JL,Fogleman JC

更新日期：1994-02-01 00:00:00

abstract：:Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Glaasker E,Smelt JP

更新日期：1998-02-01 00:00:00

abstract：:Washed cell suspensions of Desulfovibrio vulgaris rapidly reduced Cr(VI) to Cr(III) with H(2) as the electron donor. The c(3) cytochrome from this organism functioned as a Cr(VI) reductase. D. vulgaris may have advantages over previously described Cr(VI) reducers for the bioremediation of Cr(VI)-contaminated waters. ...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1994-02-01 00:00:00

abstract：:The effects of polychlorinated biphenyl (PCB) and phenanthrene stress on glucose uptake by natural microbial populations were examined by the heterotrophic potential technique. Temporal and spatial distributions in glucose uptake velocities were examined for natural samples as well as PCB- and phenanthrene-stressed sa...

journal_title：Applied and environmental microbiology

authors： Sayler GS,Lund LC,Shiaris MP,Sherrill TW,Perkins RE

更新日期：1979-05-01 00:00:00

abstract：UNLABELLED:A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalyt...

journal_title：Applied and environmental microbiology

authors： Horiuchi A,Aslam M,Kanai T,Atomi H

更新日期：2016-05-31 00:00:00

abstract：:Five rumen bacteria, Selenomonas ruminantium, Bacteroides ruminicola, Megasphaera elsdenii, Butyrivibrio fibrisolvens, and Streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. Each bacterium was grown with every substrate that it...

journal_title：Applied and environmental microbiology

authors： Russell JB,Delfino FJ,Baldwin RL

更新日期：1979-03-01 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:Shellfish-transmitted Vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the Northeast United States. This change coincided with a bacterial population shift toward human-pathogenic variants occurring in part through the introduction of several Pa...

journal_title：Applied and environmental microbiology

authors： Xu F,Gonzalez-Escalona N,Drees KP,Sebra RP,Cooper VS,Jones SH,Whistler CA

更新日期：2017-08-31 00:00:00

abstract：:Flavobacteria are a poorly understood and speciated group of commensal bacteria and opportunistic pathogens. The psychrotroph Flavobacterium psychrophilum is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic diseases that heavily impact salmonids. Consequently, two verifi...

journal_title：Applied and environmental microbiology

authors： Crump EM,Perry MB,Clouthier SC,Kay WW

更新日期：2001-02-01 00:00:00

abstract：:The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

journal_title：Applied and environmental microbiology

authors： Sasaki T,Ohshima Y

更新日期：1987-07-01 00:00:00

abstract：:Thirteen strains of Agrobacterium tumefaciens isolated from grapevine tumors in northern China were surveyed. These strains varied in their host range properties, although all were tumorigenic on grapevines. Twelve of these strains belonged to Agrobacterium sp. biotype 3, and 11 strains resulted in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Ma DQ,Yanofsky MF,Gordon MP,Nester EW

更新日期：1987-06-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabo...

journal_title：Applied and environmental microbiology

authors： Morrow KM,Bromhall K,Motti CA,Munn CB,Bourne DG

更新日期：2016-12-15 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:1,2-Dichloroethane (1,2-DCA) and 1,2-dibromoethane (ethylene dibromide [EDB]) contaminate groundwater at many hazardous waste sites. The objectives of this study were to measure yields, maximum specific growth rates (μ), and half-saturation coefficients (K(S)) in enrichment cultures that use 1,2-DCA and EDB as termina...

journal_title：Applied and environmental microbiology

authors： Yu R,Peethambaram HS,Falta RW,Verce MF,Henderson JK,Bagwell CE,Brigmon RL,Freedman DL

更新日期：2013-02-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00