Abstract:
:Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner and outer membranes. In the present study, we developed a method by which the porin AlgE was detected by immunogold labeling and transmission electron microscopy. Localization of the AlgE protein was found to depend on the presence of other proteins in the multiprotein complex. No correlation was found between the number of alginate factories and the alginate production level, nor were the numbers of these factories affected in an algC mutant that is unable to produce the precursor needed for alginate biosynthesis. Precursor availability and growth phase thus seem to be the main determinants for the alginate production rate in our strain. Clustering analysis demonstrated that the alginate multiprotein complexes were not distributed randomly over the entire outer cell membrane surface.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Maleki S,Almaas E,Zotchev S,Valla S,Ertesvåg Hdoi
10.1128/AEM.03114-15subject
Has Abstractpub_date
2015-12-11 00:00:00pages
1227-1236issue
4eissn
0099-2240issn
1098-5336pii
AEM.03114-15journal_volume
82pub_type
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更新日期:2003-11-01 00:00:00
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