Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.

Abstract:

:The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin correlated with the expression of enzymes involved in alkene and epoxide metabolism, since cell suspensions prepared from cultures grown with glucose or acetone, in which the enzymes of alkene and epoxide oxidation are not expressed, did not degrade epichlorohydrin. The alkene monooxygenase-specific inhibitor propyne had no effect on the degradation of epichlorohydrin, demonstrating that alkene monooxygenase is not involved in epichlorohydrin conversion. The interaction of epichlorohydrin and epibromohydrin with the epoxidase which catalyzes aliphatic epoxide conversions was established by showing that the epihalohydrins were specific and potent inhibitors of propylene oxide-dependent O(inf2) consumption by cell suspensions. The rates of degradation of epoxides in whole-cell suspensions decreased in the series propylene oxide > epifluorohydrin > epichlorohydrin > epibromohydrin. The pathway of epichlorohydrin degradation was investigated and found to proceed with stoichiometric dechlorination of epichlorohydrin. The first detectable product of epichlorohydrin degradation was chloroacetone. Chloroacetone was further degraded by the cell suspensions, and in the process, acetone was formed as a nonstoichiometric product. Acetone was further degraded by the cell suspensions with enzymes apparently induced by the accumulation of acetone. The metabolism of allyl chloride (3-chloropropylene) by propylene-grown cells was initiated by alkene monooxygenase and proceeded through epichlorohydrin, chloroacetone, and acetone as intermediate degradation products. These studies reveal a new pathway for halogenated epoxide degradation which involves halogenated and aliphatic ketones as well as other unidentified intermediates and which is unique from previously characterized hydrolytic degradative pathways.

journal_name

Appl Environ Microbiol

authors

Small FJ,Tilley JK,Ensign SA

doi

10.1128/AEM.61.4.1507-1513.1995

keywords:

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

1507-13

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

61

pub_type

杂志文章
  • Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.

    abstract::Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunizatio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.169-176.1982

    authors: Dahle AB,Laake M

    更新日期:1982-01-01 00:00:00

  • Next-Generation Sequencing Confirms Presumed Nosocomial Transmission of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in the Netherlands.

    abstract:UNLABELLED:Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was detected in 2003 and rapidly became the predominant MRSA clade in the Netherlands. Studies have shown that transmissions are difficult to identify, since this MRSA variant represents a genetically homogenous clade when current typ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00773-16

    authors: Bosch T,Witteveen S,Haenen A,Landman F,Schouls LM

    更新日期:2016-06-30 00:00:00

  • Comparative Toxicities of Salts on Microbial Processes in Soil.

    abstract::Soil salinization is a growing threat to global agriculture and carbon sequestration, but to date it remains unclear how microbial processes will respond. We studied the acute response to salt exposure of a range of anabolic and catabolic microbial processes, including bacterial (leucine incorporation) and fungal (ace...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04052-15

    authors: Rath KM,Maheshwari A,Bengtson P,Rousk J

    更新日期:2016-01-22 00:00:00

  • Regulation of Product Formation in Bacteroides xylanolyticus X5-1 by Interspecies Electron Transfer.

    abstract::Bacteroides xylanolyticus X5-1 was grown in pure culture and in mixed culture with Methanospirillum hungatei JF-1 under xylose limitation in the chemostat. In the pure culture, ethanol, acetate, CO(2), and hydrogen were the products. In the mixed culture, acetate, CO(2), and presumably hydrogen were the only products ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.4.1347-1352.1994

    authors: Biesterveld S,Zehnder AJ,Stams AJ

    更新日期:1994-04-01 00:00:00

  • Multimeric hemicellulases facilitate biomass conversion.

    abstract::Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02181-08

    authors: Fan Z,Wagschal K,Chen W,Montross MD,Lee CC,Yuan L

    更新日期:2009-03-01 00:00:00

  • PhaG-mediated synthesis of Poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.

    abstract::Recently, a new metabolic link between fatty acid de novo biosynthesis and biosynthesis of poly(3-hydroxy-alkanoate) consisting of medium-chain-length constituents (C(6) to C(14)) (PHA(MCL)), catalyzed by the 3-hydroxydecanoyl-[acyl-carrier-protein]:CoA transacylase (PhaG), has been identified in Pseudomonas putida (B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.2117-2124.2000

    authors: Fiedler S,Steinbüchel A,Rehm BH

    更新日期:2000-05-01 00:00:00

  • Evaluation of coliphage detection as a rapid indicator of water quality.

    abstract::A rapid coliphage analysis technique for enumerating coliphages in natural waters has been evaluated by water quality laboratories located throughout the United States. Correlations were established between coliphages and coliforms in natural water systems. These correlations were highly significant. This relationship...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.430-434.1982

    authors: Wentsel RS,O'Neill PE,Kitchens JF

    更新日期:1982-02-01 00:00:00

  • Evidence for geographic isolation and signs of endemism within a protistan morphospecies.

    abstract::The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00601-06

    authors: Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

    更新日期:2006-08-01 00:00:00

  • Attached Growth of Sphaerotilus natans in Continuous-Flow Apparatus and Its Growth Inhibition by 9-beta-d-Arabinofuranosyladenine.

    abstract::Sphaerotilus natans grew at the maximum specific growth rate (mu(max)) of 0.43/h when cultivated on PGY medium at 25 degrees C. The organism mainly grew attached to inside of the culture vessels when the culture medium was fed to the completely mixed continuous-flow apparatus at a dilution rate above mu(max) and the a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.2.200-204.1979

    authors: Yoshikawa H,Takiguchi Y

    更新日期:1979-08-01 00:00:00

  • Streptococcus thermophilus is able to produce a beta-galactosidase active during its transit in the digestive tract of germ-free mice.

    abstract::This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.938-941.2002

    authors: Drouault S,Anba J,Corthier G

    更新日期:2002-02-01 00:00:00

  • Evaluation of MK filters for recovery of enteroviruses from tap water.

    abstract::The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.6.1974-1977.1994

    authors: Ma JF,Naranjo J,Gerba CP

    更新日期:1994-06-01 00:00:00

  • Production of Eremofortins A, B, and C Relative to Formation of PR Toxin by Penicillium roqueforti.

    abstract::The production of various eremophilane-type sesquiterpenes by Penicillium roqueforti strains has allowed us to propose a biochemical pathway for PR toxin synthesis. A time-course study of P. roqueforti metabolite production by high-performance liquid chromatography was performed to check this hypothetical pathway. The...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.4.770-776.1980

    authors: Moreau S,Lablache-Combier A,Biguet J

    更新日期:1980-04-01 00:00:00

  • Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis.

    abstract::Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.3.545-549.1984

    authors: Kakoyiannis CK,Winter PJ,Marshall RB

    更新日期:1984-09-01 00:00:00

  • Poly-N-acetylglucosamine matrix polysaccharide impedes fluid convection and transport of the cationic surfactant cetylpyridinium chloride through bacterial biofilms.

    abstract::Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01900-08

    authors: Ganeshnarayan K,Shah SM,Libera MR,Santostefano A,Kaplan JB

    更新日期:2009-03-01 00:00:00

  • Rapid PCR-based method which can determine both phenotype and genotype of Lactococcus lactis subspecies.

    abstract::A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.5.2209-2213.2002

    authors: Nomura M,Kobayashi M,Okamoto T

    更新日期:2002-05-01 00:00:00

  • Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons.

    abstract::A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03048-10

    authors: Ganguli PS,Chen W,Yates MV

    更新日期:2011-08-01 00:00:00

  • Temperature-driven adaptation of the bacterial community in peat measured by using thymidine and leucine incorporation.

    abstract::The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1116-1122.2001

    authors: Ranneklev SB,Bååth E

    更新日期:2001-03-01 00:00:00

  • Host exopolysaccharide quantity and composition impact Erwinia amylovora bacteriophage pathogenesis.

    abstract::Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00067-13

    authors: Roach DR,Sjaarda DR,Castle AJ,Svircev AM

    更新日期:2013-05-01 00:00:00

  • Isolation and characterization of Micromonospora phage PhiHAU8 and development into a phasmid.

    abstract::PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Dif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.3893-3897.2004

    authors: Li X,Zhou X,Deng Z

    更新日期:2004-07-01 00:00:00

  • Gene cloning, purification, and characterization of a novel peptidoglutaminase-asparaginase from Aspergillus sojae.

    abstract::Glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. By using the genome sequence and expressed sequence tag data available for Aspergillus oryzae RIB40, we cloned a novel glutaminase gene...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00765-12

    authors: Ito K,Matsushima K,Koyama Y

    更新日期:2012-08-01 00:00:00

  • Enhanced inactivation of Salmonella and Pseudomonas biofilms on stainless steel by use of T-128, a fresh-produce washing aid, in chlorinated wash solutions.

    abstract::The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01094-12

    authors: Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

    更新日期:2012-10-01 00:00:00

  • Reduction of Chromate by Desulfovibrio vulgaris and Its c(3) Cytochrome.

    abstract::Washed cell suspensions of Desulfovibrio vulgaris rapidly reduced Cr(VI) to Cr(III) with H(2) as the electron donor. The c(3) cytochrome from this organism functioned as a Cr(VI) reductase. D. vulgaris may have advantages over previously described Cr(VI) reducers for the bioremediation of Cr(VI)-contaminated waters. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.2.726-728.1994

    authors: Lovley DR,Phillips EJ

    更新日期:1994-02-01 00:00:00

  • Secretion of cryparin, a fungal hydrophobin.

    abstract::Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5431-5435.1999

    authors: McCabe PM,Van Alfen NK

    更新日期:1999-12-01 00:00:00

  • Microbiota-Mediated Modulation of Organophosphate Insecticide Toxicity by Species-Dependent Interactions with Lactobacilli in a Drosophila melanogaster Insect Model.

    abstract::Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02820-17

    authors: Daisley BA,Trinder M,McDowell TW,Collins SL,Sumarah MW,Reid G

    更新日期:2018-04-16 00:00:00

  • Investigation of malic acid production in Aspergillus oryzae under nitrogen starvation conditions.

    abstract::Malic acid has great potential for replacing petrochemical building blocks in the future. For this application, high yields, rates, and titers are essential in order to sustain a viable biotechnological production process. Natural high-capacity malic acid producers like the malic acid producer Aspergillus flavus have ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01445-13

    authors: Knuf C,Nookaew I,Brown SH,McCulloch M,Berry A,Nielsen J

    更新日期:2013-10-01 00:00:00

  • Expression of a tetraheme protein, Desulfovibrio vulgaris Miyazaki F cytochrome c(3), in Shewanella oneidensis MR-1.

    abstract::Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.4168-4171.2000

    authors: Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

    更新日期:2000-09-01 00:00:00

  • Presence of enterohemorrhagic Escherichia coli ST678/O104:H4 in France prior to 2011.

    abstract::Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06524-11

    authors: Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

    更新日期:2011-12-01 00:00:00

  • OusB, a broad-specificity ABC-type transporter from Erwinia chrysanthemi, mediates uptake of glycine betaine and choline with a high affinity.

    abstract::The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolate...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.7.3389-3398.2005

    authors: Choquet G,Jehan N,Pissavin C,Blanco C,Jebbar M

    更新日期:2005-07-01 00:00:00

  • Microbial reduction of Fe(III) in acidic sediments: isolation of Acidiphilium cryptum JF-5 capable of coupling the reduction of Fe(III) to the oxidation of glucose.

    abstract::To evaluate the microbial populations involved in the reduction of Fe(III) in an acidic, iron-rich sediment, the anaerobic flow of supplemental carbon and reductant was evaluated in sediment microcosms at the in situ temperature of 12 degrees C. Supplemental glucose and cellobiose stimulated the formation of Fe(II); 4...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.8.3633-3640.1999

    authors: Küsel K,Dorsch T,Acker G,Stackebrandt E

    更新日期:1999-08-01 00:00:00

  • Spatial relationship of the vaginal microflora to the vaginal epithelium of female rats: scanning electron microscopy.

    abstract::The vaginal mucosa of ovariectomized female rats has been examined by scanning electron microscopy before and after estrogen [1,3,5(10)-estratriene-3,17beta-diol] treatment. Without estrogen stimulation vaginal colonization is minimal and the epithelium is characterized by a layer of epithelial cells covered with smal...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.1.80-87.1977

    authors: Larsen B,Markovetz AJ,Galask RP

    更新日期:1977-07-01 00:00:00