PhaG-mediated synthesis of Poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.

abstract：:A new compound, designated ML-449, structurally similar to the known 20-membered macrolactam BE-14106, was isolated from a marine sediment-derived Streptomyces sp. Cloning and sequencing of the 83-kb ML-449 biosynthetic gene cluster revealed its high level of similarity to the BE-14106 gene cluster. Comparison of the ...

journal_title：Applied and environmental microbiology

authors： Jørgensen H,Degnes KF,Dikiy A,Fjaervik E,Klinkenberg G,Zotchev SB

更新日期：2010-01-01 00:00:00

abstract：:Laboratory experiments have definitively shown that exopolymer-producing bacteria have the potential to modify the flow of fluids in oil reservoirs to enhance oil production. Once injected into the reservoir, they will be subjected to a wide range of pH values and to starvation resulting from nutrient depletion. For s...

journal_title：Applied and environmental microbiology

authors： Kim DS,Thomas S,Fogler HS

更新日期：2000-03-01 00:00:00

abstract：:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (b...

journal_title：Applied and environmental microbiology

authors： Ceragioli M,Mols M,Moezelaar R,Ghelardi E,Senesi S,Abee T

更新日期：2010-05-01 00:00:00

abstract：:When mixed ruminal bacteria were incubated with a pancreatic casein hydrolysate and free amino acids of a similar composition, rates of ammonia production were much greater for peptides than for amino acids. The pancreatic digest of casein was then fractionated with 90% isopropyl alcohol. Hydrophobic peptides which di...

journal_title：Applied and environmental microbiology

authors： Chen G,Strobel HJ,Russell JB,Sniffen CJ

更新日期：1987-09-01 00:00:00

abstract：:Campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in Sydney, Australia. Three of four flocks examined carried C. jejuni. Eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. ...

journal_title：Applied and environmental microbiology

authors： Shanker S,Rosenfield JA,Davey GR,Sorrell TC

更新日期：1982-05-01 00:00:00

abstract：:The products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of Pseudomonas fluorescens Pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. Five analogs of rhizoxin, a 16-member macrolide w...

journal_title：Applied and environmental microbiology

authors： Loper JE,Henkels MD,Shaffer BT,Valeriote FA,Gross H

更新日期：2008-05-01 00:00:00

abstract：:Three species of colonic bacteria can ferment the mucopolysaccharide chondroitin sulfate: Bacteroides ovatus, Bacteroides sp. strain 3452A (an unnamed DNA homology group), and B. thetaiotaomicron. Proteins associated with the utilization of chondroitin sulfate by B. thetaiotaomicron have been characterized previously....

journal_title：Applied and environmental microbiology

authors： Lipeski L,Guthrie EP,O'Brien M,Kotarski SF,Salyers AA

更新日期：1986-05-01 00:00:00

abstract：:Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

journal_title：Applied and environmental microbiology

authors： Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

更新日期：2014-02-01 00:00:00

abstract：:To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

journal_title：Applied and environmental microbiology

authors： Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

更新日期：2020-01-21 00:00:00

abstract：:Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. s...

journal_title：Applied and environmental microbiology

authors： Asaka O,Shoda M

更新日期：1996-11-01 00:00:00

abstract：:Bacteriovorous protozoa harboring symbiotic algae are abundant in aquatic ecosystems, yet despite a recent interest in protozoan bacterivory, the influence of light on their ingestion rates has not been investigated. In this study, Paramecium bursaria containing endosymbiotic Chlorella was tested for the effect of lig...

journal_title：Applied and environmental microbiology

authors： Berk SG,Parks LH,Ting RS

更新日期：1991-08-01 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:A unique lipolytic bacterium was isolated in a selective growth system consisting of 99% triglycerides and a 1% water phase. The bacterium, termed Pseudomonas aeruginosa YS-7, was able to grow in an environment of low water content and could also survive amphipathic, osmotic, and matrical water stress in a triglycerid...

journal_title：Applied and environmental microbiology

authors： Shabtai Y

更新日期：1991-06-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains ...

journal_title：Applied and environmental microbiology

authors： Wozny MA,Bryant MP,Holdeman LV,Moore WE

更新日期：1977-05-01 00:00:00

abstract：:The U.S. Environmental Protection Agency's information collection rule requires the use of 1MDS electropositive filters for concentrating enteric viruses from water, but unfortunately, these filters are not cost-effective for routine viral monitoring. In this study, an inexpensive electropositive cartridge filter, the...

journal_title：Applied and environmental microbiology

authors： Karim MR,Rhodes ER,Brinkman N,Wymer L,Fout GS

更新日期：2009-04-01 00:00:00

abstract：:A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

journal_title：Applied and environmental microbiology

authors： Choi Y,Shin H,Lee JH,Ryu S

更新日期：2013-08-01 00:00:00

abstract：:Suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees C were subjected to smoke from four cigarettes over a 4-h period. The cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. The virus suspensions were then titrated...

journal_title：Applied and environmental microbiology

authors： Bardell D

更新日期：1978-11-01 00:00:00

abstract：:Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yi...

journal_title：Applied and environmental microbiology

authors： Grant MA

更新日期：2004-02-01 00:00:00

abstract：:Facultative endosymbionts can benefit insect hosts in a variety of ways, including context-dependent roles, such as providing defense against pathogens. The role of some symbionts in defense may be overlooked, however, when pathogen infection is transient, sporadic, or asymptomatic. The facultative endosymbiont Ricket...

journal_title：Applied and environmental microbiology

authors： Hendry TA,Hunter MS,Baltrus DA

更新日期：2014-12-01 00:00:00

abstract：:Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH,...

journal_title：Applied and environmental microbiology

authors： Wilkins JC,Homer KA,Beighton D

更新日期：2001-08-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:Chromohalobacter salexigens DSM 3043 can grow on N,N-dimethylglycine (DMG) as the sole C, N, and energy source and utilize sarcosine as the sole N source under aerobic conditions. However, little is known about the genes and enzymes involved in the conversion of DMG to sarcosine in this strain. In the present study, g...

journal_title：Applied and environmental microbiology

authors： Yang T,Shao YH,Guo LZ,Meng XL,Yu H,Lu WD

更新日期：2020-08-18 00:00:00

abstract：:The currently used microbial decontamination method for spacecraft and components uses dry-heat microbial reduction at temperatures of >110°C for extended periods to prevent the contamination of extraplanetary destinations. This process is effective and reproducible, but it is also long and costly and precludes the us...

journal_title：Applied and environmental microbiology

authors： Pottage T,Macken S,Giri K,Walker JT,Bennett AM

更新日期：2012-06-01 00:00:00

abstract：:The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximatel...

journal_title：Applied and environmental microbiology

authors： Senko JM,Stolz JF

更新日期：2001-08-01 00:00:00

abstract：:The genus Sepiola (Cephalopoda: Sepiolidae) contains 10 known species that occur in the Mediterranean Sea today. All Sepiola species have a light organ that contains at least one of two species of luminous bacteria, Vibrio fischeri and Vibrio logei. The two Vibrio species coexist in at least four Sepiola species (S. a...

journal_title：Applied and environmental microbiology

authors： Nishiguchi MK

更新日期：2000-08-01 00:00:00

abstract：:Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plas...

journal_title：Applied and environmental microbiology

authors： Picardeau M

更新日期：2008-01-01 00:00:00

abstract：:Streptomyces subtilisin inhibitor-like proteins were found to be distributed widely in streptomycetes by using the combination of the convenient, newly developed plate assay system and an established liquid culture assay. Almost all the strains formerly categorized as Streptoverticillium species produced proteins that...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Kikuchi H,Suzuki M,Kojima S,Terabe M,Miura K,Nakase T,Momose H

更新日期：1993-12-01 00:00:00

abstract：:Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (...

journal_title：Applied and environmental microbiology

authors： Délye C,Ronchi V,Laigret F,Corio-Costet MF

更新日期：1999-09-01 00:00:00

abstract：:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation mi...

journal_title：Applied and environmental microbiology

authors： Rizzi A,Leroux J,Charron-Lamoureux V,Roy S,Beauregard PB,Bellenger JP

更新日期：2020-10-28 00:00:00