PhaG-mediated synthesis of Poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.

abstract：:Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that inf...

journal_title：Applied and environmental microbiology

authors： Whistler CA,Stockwell VO,Loper JE

更新日期：2000-07-01 00:00:00

abstract：:In this study, the effect of individual lecithin phospholipids on the antimicrobial properties of eugenol against Escherichia coli C600 was investigated. We tested five major phospholipids common in soy or egg lecithin (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine [DPPC], 1,2-dioctadecanoyl-sn-glycero-3-phosphocholi...

journal_title：Applied and environmental microbiology

authors： Zhang H,Dudley EG,Harte F

更新日期：2017-10-17 00:00:00

abstract：:A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...

journal_title：Applied and environmental microbiology

authors： Park S,Navratil S,Gregory A,Bauer A,Srinath I,Szonyi B,Nightingale K,Anciso J,Jun M,Han D,Lawhon S,Ivanek R

更新日期：2015-04-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:In this study, multilocus sequence typing (MLST) was combined with the genetic detection of six genetic markers, ansB, dmsA, ggt, cj1585c, cjj81176-1367/71 (cj1365c), and the two-gene marker tlp7 (cj0951c plus cj0952c), to assess if their presence correlated with different C. jejuni clonal groups. Using a collection o...

journal_title：Applied and environmental microbiology

authors： Zautner AE,Herrmann S,Corso J,Tareen AM,Alter T,Gross U

更新日期：2011-04-01 00:00:00

abstract：:To cause an infection, bacteriophages must penetrate the alginate exopolysaccharide of Pseudomonas aeruginosa to reach the bacterial surface. Despite a lack of intrinsic motility, phage were shown to diffuse through alginate gels at alginate concentrations up to 8% (wt/vol) and to bring about a 2-log reduction in the ...

journal_title：Applied and environmental microbiology

authors： Hanlon GW,Denyer SP,Olliff CJ,Ibrahim LJ

更新日期：2001-06-01 00:00:00

abstract：:Streptococcus thermophilus is a facultative anaerobic bacterium that has the ability to grow and survive in aerobic environments, but the mechanism for this remains unclear. In this study, the efeB gene, encoding a dye-decolorizing peroxidase, was identified in the genome of Streptococcus thermophilus CGMCC 7.179, and...

journal_title：Applied and environmental microbiology

authors： Zhang C,Xin Y,Wang Y,Guo T,Lu S,Kong J

更新日期：2015-09-01 00:00:00

abstract：:Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

journal_title：Applied and environmental microbiology

authors： Arthurs CE,Lloyd D

更新日期：1999-08-01 00:00:00

abstract：:An immunoblot procedure for the strain-specific quantitative analysis of commercial Rhizobium inoculants was developed. The technique greatly reduced the time required for inoculant analysis. Correlation between immunoblot analysis and traditional plant nodule grow-out most-probable-number techniques was r = 0.90 for ...

journal_title：Applied and environmental microbiology

authors： Olsen PE,Rice WA

更新日期：1989-02-01 00:00:00

abstract：:The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both...

journal_title：Applied and environmental microbiology

authors： Li B,Nagalla SR,Renganathan V

更新日期：1997-02-01 00:00:00

abstract：:Swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. This study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions...

journal_title：Applied and environmental microbiology

authors： Abdelzaher AM,Wright ME,Ortega C,Solo-Gabriele HM,Miller G,Elmir S,Newman X,Shih P,Bonilla JA,Bonilla TD,Palmer CJ,Scott T,Lukasik J,Harwood VJ,McQuaig S,Sinigalliano C,Gidley M,Plano LR,Zhu X,Wang JD,Fleming LE

更新日期：2010-02-01 00:00:00

abstract：:Filamentous, gliding, sulfide-oxidizing bacteria of the genus Thioploca were found on sediments in profundal areas of Lake Biwa, a Japanese freshwater mesotrophic lake, and were characterized morphologically and phylogenetically. The Lake Biwa Thioploca resembled morphologically Thioploca ingrica, a brackish water spe...

journal_title：Applied and environmental microbiology

authors： Kojima H,Teske A,Fukui M

更新日期：2003-01-01 00:00:00

abstract：:Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus f...

journal_title：Applied and environmental microbiology

authors： Solheim M,Aakra A,Vebø H,Snipen L,Nes IF

更新日期：2007-09-01 00:00:00

abstract：:Pseudomonas aureofaciens PGS12 produces three phenazine antibiotics, in addition to siderophores, hydrogen cyanide, pyrrolnitrin, and indoleacetic acid. Tn5-259.7 transposon mutagenesis was carried out to identify and clone a chromosomal locus involved in phenazine biosynthesis. Three classes of mutants were obtained:...

journal_title：Applied and environmental microbiology

authors： Georgakopoulos DG,Hendson M,Panopoulos NJ,Schroth MN

更新日期：1994-08-01 00:00:00

abstract：:Lactococcus lactis subsp. cremoris MG1363 is a model for the lactic acid bacteria (LAB) used in the dairy industry. The proteolytic system, consisting of a proteinase, several peptide and amino acid uptake systems, and a host of intracellular peptidases, plays a vital role in nitrogen metabolism and is of eminent impo...

journal_title：Applied and environmental microbiology

authors： Huang C,Hernandez-Valdes JA,Kuipers OP,Kok J

更新日期：2020-04-01 00:00:00

abstract：:This study quantified the levels of airborne microorganisms in six swine farms with more than 10,000 pigs in subtropical Taiwan. We evaluated breeding, growing, and finishing stalls, which were primarily open-air buildings, as well as partially enclosed farrowing and nursery piggeries. Airborne culturable bacteria, gr...

journal_title：Applied and environmental microbiology

authors： Chang CW,Chung H,Huang CF,Su HJ

更新日期：2001-01-01 00:00:00

abstract：:In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsib...

journal_title：Applied and environmental microbiology

authors： Bao Z,Okubo T,Kubota K,Kasahara Y,Tsurumaru H,Anda M,Ikeda S,Minamisawa K

更新日期：2014-08-01 00:00:00

abstract：:The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invad...

journal_title：Applied and environmental microbiology

authors： Hudault S,Liévin V,Bernet-Camard MF,Servin AL

更新日期：1997-02-01 00:00:00

abstract：:Batch cultures of a lithotrophic Fe(II)-oxidizing bacterium, strain BrT, isolated from the rhizosphere of a wetland plant, were grown in bioreactors and used to determine the significance of microbial Fe(II) oxidation at circumneutral pH and to identify abiotic variables that affect the partitioning between microbial ...

journal_title：Applied and environmental microbiology

authors： Neubauer SC,Emerson D,Megonigal JP

更新日期：2002-08-01 00:00:00

abstract：:Alkyl glycosides are attractive surfactants because of their high surface activity and good biodegradability and can be produced from renewable resources. Through enzymatic catalysis, one can obtain well-defined alkyl glycosides, something that is very difficult to do using conventional chemistry. However, there is a ...

journal_title：Applied and environmental microbiology

authors： Lundemo P,Adlercreutz P,Karlsson EN

更新日期：2013-06-01 00:00:00

abstract：:The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

journal_title：Applied and environmental microbiology

authors： Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

更新日期：2007-05-01 00:00:00

abstract：:A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...

journal_title：Applied and environmental microbiology

authors： Yurkov V,Beatty JT

更新日期：1998-01-01 00:00:00

abstract：:A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

journal_title：Applied and environmental microbiology

authors： Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

更新日期：2004-10-01 00:00:00

abstract：:Cellular fatty acids of 10 strains of lactic acid bacteria were analyzed. The purpose of this work was to find lactic acid bacteria with high lactobacillic acid contents. The bacteria studied were unable to synthesize oleic acid. Some strains did not synthesize lactobacillic acid, although all were able to form dihydr...

journal_title：Applied and environmental microbiology

authors： Johnsson T,Nikkila P,Toivonen L,Rosenqvist H,Laakso S

更新日期：1995-12-01 00:00:00

abstract：:The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

journal_title：Applied and environmental microbiology

authors： Garcia-Pichel F,Wingard CE,Castenholz RW

更新日期：1993-01-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:It is now possible to obtain accurate total counts of the bacteria of natural waters with the use of acridine orange staining and epifluorescence microscopy. This approach can be coupled to highly sensitive measurements of heterotrophic activity using radioisotopes. To accomplish this, three variations of a "specific ...

journal_title：Applied and environmental microbiology

authors： Wright RT

更新日期：1978-08-01 00:00:00

abstract：:Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotro...

journal_title：Applied and environmental microbiology

authors： Londry KL,Des Marais DJ

更新日期：2003-05-01 00:00:00

abstract：:Protoplasts of the monokaryotic strain 52J of Trametes versicolor were treated with UV light and screened for the inability to produce a colored precipitate on guaiacol-containing agar plates. Mutants unable to oxidize guaiacol had absent or very low secretion of laccase and manganese peroxidase (MnP) proteins. All is...

journal_title：Applied and environmental microbiology

authors： Addleman K,Dumonceaux T,Paice MG,Bourbonnais R,Archibald FS

更新日期：1995-10-01 00:00:00