Isolation and characterization of Micromonospora phage PhiHAU8 and development into a phasmid.

Abstract:

:PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Difco nutrient broth within a pH range of 6 to 12. PhiHAU8 plaque formation on Micromonospora sp. strain 40027 was optimal with 32 mM Ca(2+) and 30 mM Mg(2+). A lysogen, LXH8, was isolated from turbid plaques, and a phasmid derivative that functions as a lambda cosmid vector in Escherichia coli and as a phage in Micromonospora sp. strain 40027 was constructed. Pulsed-field gel electrophoresis of AseI-digested total DNA showed that PhiHAU8 DNA integrates into the 500-kb AseI fragment of Micromonospora sp. strain 40027.

journal_name

Appl Environ Microbiol

authors

Li X,Zhou X,Deng Z

doi

10.1128/AEM.70.7.3893-3897.2004

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

3893-7

issue

7

eissn

0099-2240

issn

1098-5336

pii

70/7/3893

journal_volume

70

pub_type

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