Abstract:
:PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Difco nutrient broth within a pH range of 6 to 12. PhiHAU8 plaque formation on Micromonospora sp. strain 40027 was optimal with 32 mM Ca(2+) and 30 mM Mg(2+). A lysogen, LXH8, was isolated from turbid plaques, and a phasmid derivative that functions as a lambda cosmid vector in Escherichia coli and as a phage in Micromonospora sp. strain 40027 was constructed. Pulsed-field gel electrophoresis of AseI-digested total DNA showed that PhiHAU8 DNA integrates into the 500-kb AseI fragment of Micromonospora sp. strain 40027.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Li X,Zhou X,Deng Zdoi
10.1128/AEM.70.7.3893-3897.2004keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
3893-7issue
7eissn
0099-2240issn
1098-5336pii
70/7/3893journal_volume
70pub_type
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