Toxic effects of fermented and unfermented sorghum meal diets naturally contaminated with mycotoxins.

abstract：:Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

journal_title：Applied and environmental microbiology

authors： Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

更新日期：2008-02-01 00:00:00

abstract：:The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

journal_title：Applied and environmental microbiology

authors： Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

更新日期：2002-01-01 00:00:00

abstract：:Predicting the presence of enteric viruses in surface waters is a complex modeling problem. Multiple water quality parameters that indicate the presence of human fecal material, the load of fecal material, and the amount of time fecal material has been in the environment are needed. This paper presents the results of ...

journal_title：Applied and environmental microbiology

authors： Black LE,Brion GM,Freitas SJ

更新日期：2007-06-01 00:00:00

abstract：:Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

journal_title：Applied and environmental microbiology

authors： Liu W,Hansen JN

更新日期：1990-08-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

journal_title：Applied and environmental microbiology

authors： Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

更新日期：1987-08-01 00:00:00

abstract：:The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a sclerotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zo...

journal_title：Applied and environmental microbiology

authors： Wicklow DT,Joshi BK,Gamble WR,Gloer JB,Dowd PF

更新日期：1998-11-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

journal_title：Applied and environmental microbiology

authors： Huber H,Stetter KO

更新日期：1990-02-01 00:00:00

abstract：:Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

journal_title：Applied and environmental microbiology

authors： Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

更新日期：2006-04-01 00:00:00

abstract：UNLABELLED:Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in a healthy population. We conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of Bifidobacterium bifidum strain Bb on the IME of adult hea...

journal_title：Applied and environmental microbiology

authors： Gargari G,Taverniti V,Balzaretti S,Ferrario C,Gardana C,Simonetti P,Guglielmetti S

更新日期：2016-09-16 00:00:00

abstract：:Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

journal_title：Applied and environmental microbiology

authors： Schesser K,Luder A,Henson JM

更新日期：1991-02-01 00:00:00

abstract：:Newcastle disease, one of the most important health problems that affects the poultry industry around the world, is caused by virulent strains of Newcastle disease virus. Newcastle disease virus is considered to be endemic in several countries in the Americas, including Mexico. In order to control Newcastle disease ou...

journal_title：Applied and environmental microbiology

authors： Cardenas Garcia S,Navarro Lopez R,Morales R,Olvera MA,Marquez MA,Merino R,Miller PJ,Afonso CL

更新日期：2013-08-01 00:00:00

abstract：:Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Sa...

journal_title：Applied and environmental microbiology

authors： Kim HJ,Park SH,Kim HY

更新日期：2006-09-01 00:00:00

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, wh...

journal_title：Applied and environmental microbiology

authors： Coletta-Filho HD,Takita MA,de Souza AA,Aguilar-Vildoso CI,Machado MA

更新日期：2001-09-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：:Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the ...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McConville M,de Jong A

更新日期：2006-06-01 00:00:00

abstract：:A new thermostable dipeptidase gene was cloned from the thermophile Brevibacillus borstelensis BCS-1 by genetic complementation of the D-Glu auxotroph Escherichia coli WM335 on a plate containing D-Ala-D-Glu. Nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid...

journal_title：Applied and environmental microbiology

authors： Baek DH,Song JJ,Kwon SJ,Park C,Jung CM,Sung MH

更新日期：2004-03-01 00:00:00

abstract：:While the contribution of Bacteria to bioremediation of oil-contaminated shorelines is well established, the response of Archaea to spilled oil and bioremediation treatments is unknown. The relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bio...

journal_title：Applied and environmental microbiology

authors： Röling WF,de Brito Couto IR,Swannell RP,Head IM

更新日期：2004-05-01 00:00:00

abstract：:Campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in Sydney, Australia. Three of four flocks examined carried C. jejuni. Eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. ...

journal_title：Applied and environmental microbiology

authors： Shanker S,Rosenfield JA,Davey GR,Sorrell TC

更新日期：1982-05-01 00:00:00

abstract：:To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

journal_title：Applied and environmental microbiology

authors： Richter D,Schlee DB,Allgöwer R,Matuschka FR

更新日期：2004-11-01 00:00:00

abstract：:Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE,Rodrick GE

更新日期：1981-02-01 00:00:00

abstract：:We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex geno...

journal_title：Applied and environmental microbiology

authors： Wang S,He J,Cui Z,Li S

更新日期：2007-08-01 00:00:00

abstract：:Pfiesteria piscicida is a heterotrophic dinoflagellate widely distributed along the middle Atlantic shore of the United States and associated with fish kills in the Neuse River (North Carolina) and the Chesapeake Bay (Maryland and Virginia). We constructed a genomic DNA library from clonally cultured P. piscicida and ...

journal_title：Applied and environmental microbiology

authors： Saito K,Drgon T,Robledo JA,Krupatkina DN,Vasta GR

更新日期：2002-11-01 00:00:00

abstract：:More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

journal_title：Applied and environmental microbiology

authors： Hahn MW

更新日期：2003-09-01 00:00:00

abstract：:There are a number of genetic tools available for studying Francisella tularensis, the etiological agent of tularemia; however, there is no effective inducible or repressible gene expression system. Here, we describe inducible and repressible gene expression systems for F. tularensis based on the Tet repressor, TetR. ...

journal_title：Applied and environmental microbiology

authors： LoVullo ED,Miller CN,Pavelka MS Jr,Kawula TH

更新日期：2012-10-01 00:00:00

abstract：:A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...

journal_title：Applied and environmental microbiology

authors： Nakamura K,Nakahara H

更新日期：1988-11-01 00:00:00

abstract：:A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...

journal_title：Applied and environmental microbiology

authors： Jackman DM,Bartlett FM,Patel TR

更新日期：1983-07-01 00:00:00

abstract：:Although Pelobacter species are closely related to Geobacter species, recent studies suggested that Pelobacter carbinolicus may reduce Fe(III) via a different mechanism because it lacks the outer-surface c-type cytochromes that are required for Fe(III) reduction by Geobacter sulfurreducens. Investigation into the mech...

journal_title：Applied and environmental microbiology

authors： Haveman SA,DiDonato RJ Jr,Villanueva L,Shelobolina ES,Postier BL,Xu B,Liu A,Lovley DR

更新日期：2008-07-01 00:00:00