Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in Central Europe.

abstract：:The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

journal_title：Applied and environmental microbiology

authors： Small FJ,Tilley JK,Ensign SA

更新日期：1995-04-01 00:00:00

abstract：:The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter up...

journal_title：Applied and environmental microbiology

authors： Soltes-Rak E,Kushner DJ,Williams DD,Coleman JR

更新日期：1993-08-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:Purified manganese peroxidase (MnP) from the white-rot basidiomycete Phlebia radiata was found to convert in vitro milled pine wood (MPW) suspended in an aqueous reaction solution containing Tween 20, Mn(2+), Mn-chelating organic acid (malonate), and a hydrogen peroxide-generating system (glucose-glucose oxidase). The...

journal_title：Applied and environmental microbiology

authors： Hofrichter M,Lundell T,Hatakka A

更新日期：2001-10-01 00:00:00

abstract：:Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

journal_title：Applied and environmental microbiology

authors： Vannelli T,Hooper AB

更新日期：1992-07-01 00:00:00

abstract：:A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

journal_title：Applied and environmental microbiology

authors： Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

更新日期：2015-12-01 00:00:00

abstract：:The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicro...

journal_title：Applied and environmental microbiology

authors： Scupham AJ,Jones JA,Rettedal EA,Weber TE

更新日期：2010-12-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:An efficient insertion mutagenesis strategy for bacterial genomes based on the phage Mu DNA transposition reaction was developed. Incubation of MuA transposase protein with artificial mini-Mu transposon DNA in the absence of divalent cations in vitro resulted in stable but inactive Mu DNA transposition complexes, or t...

journal_title：Applied and environmental microbiology

authors： Lamberg A,Nieminen S,Qiao M,Savilahti H

更新日期：2002-02-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Curr...

journal_title：Applied and environmental microbiology

authors： Tseng M,Fratamico PM,Bagi L,Delannoy S,Fach P,Manning SD,Funk JA

更新日期：2014-10-01 00:00:00

abstract：:Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...

journal_title：Applied and environmental microbiology

authors： Poppert S,Essig A,Marre R,Wagner M,Horn M

更新日期：2002-08-01 00:00:00

abstract：:Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

journal_title：Applied and environmental microbiology

authors： Brown JC,Huang G,Haley-Zitlin V,Jiang X

更新日期：2009-02-01 00:00:00

abstract：:2-Bromoethanesulfonic acid (BESA) and 2-chloroethanesulfonic acid (CESA) have been reported to be potent inhibitors of methane formation during methanogenic decomposition in batch cultures. However, in a laboratory-scale continuous-flow methanogenic fixed-film column containing a predominance of acetate-decarboxylatin...

journal_title：Applied and environmental microbiology

authors： Bouwer EJ,McCarty PL

更新日期：1983-04-01 00:00:00

abstract：:Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

journal_title：Applied and environmental microbiology

authors： Klinke S,Dauner M,Scott G,Kessler B,Witholt B

更新日期：2000-03-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...

journal_title：Applied and environmental microbiology

authors： Park SY,Choi SK,Kim J,Oh TK,Park SH

更新日期：2012-06-01 00:00:00

abstract：:A seasonal study of the distribution of lysogenic bacteria in Tampa Bay, Florida, was conducted over a 13-month period. Biweekly water samples were collected and either were left unaltered or had the viral population reduced by filtration (pore size, 0.2 micro m) and resuspension in filtered (pore size, 0.2 micro m) w...

journal_title：Applied and environmental microbiology

authors： Williamson SJ,Houchin LA,McDaniel L,Paul JH

更新日期：2002-09-01 00:00:00

abstract：:A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of...

journal_title：Applied and environmental microbiology

authors： Heipieper HJ,Diefenbach R,Keweloh H

更新日期：1992-06-01 00:00:00

abstract：:Relatedness between Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis was assessed by Southern hybridization analysis, with cloned chromosomal genes as probes. The results indicate that strains of the two subspecies form two distinct groups and that the DNA sequence divergence between L. lactis subsp. lac...

journal_title：Applied and environmental microbiology

authors： Godon JJ,Delorme C,Ehrlich SD,Renault P

更新日期：1992-12-01 00:00:00

abstract：:A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

journal_title：Applied and environmental microbiology

authors： Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

更新日期：2013-03-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:Bacteriovorous protozoa harboring symbiotic algae are abundant in aquatic ecosystems, yet despite a recent interest in protozoan bacterivory, the influence of light on their ingestion rates has not been investigated. In this study, Paramecium bursaria containing endosymbiotic Chlorella was tested for the effect of lig...

journal_title：Applied and environmental microbiology

authors： Berk SG,Parks LH,Ting RS

更新日期：1991-08-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...

journal_title：Applied and environmental microbiology

authors： Erdman MD,Bergen WG,Reddy CA

更新日期：1977-04-01 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization...

journal_title：Applied and environmental microbiology

authors： Burggraf S,Mayer T,Amann R,Schadhauser S,Woese CR,Stetter KO

更新日期：1994-09-01 00:00:00