A virulent phage infecting Lactococcus garvieae, with homology to Lactococcus lactis phages.

abstract：:The differential resistance of substratum-attached, detached, and planktonic cells of Salmonella enteritidis phage type 4 was studied by using several inimical processes and in vivo bioluminescence as a nondestructive, real-time reporter of metabolic activity. Bioluminescence in this strain was mediated by a construct...

journal_title：Applied and environmental microbiology

authors： Dhir VK,Dodd CE

更新日期：1995-05-01 00:00:00

abstract：:The production of a suitable substrate for the cultivation of the common white button mushroom, Agaricus bisporus, is referred to as composting. High microbiological activity causes temperatures of the composting material to rise as high as 80 degrees C. At stacking, an optimal oxygen consumption rate of 140 mumol of ...

journal_title：Applied and environmental microbiology

authors： Derikx PJ,Op Den Camp HJ,van der Drift C,Van Griensven LJ,Vogels GD

更新日期：1990-10-01 00:00:00

abstract：:The killing of Listeria monocytogenes following exposure to low pH, organic acids, and osmotic stress was enhanced by the addition of 5% (vol/vol) ethanol. At pH 3, for example, the presence of this agent stimulated killing by more than 3 log units in 40 min of exposure. The rate of cell death at pH 3.0 was dependent ...

journal_title：Applied and environmental microbiology

authors： Barker C,Park SF

更新日期：2001-04-01 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:Experiments were carried out to examine the behavior of Listeria monocytogenes in the course of fabrication and storage of smoked salmon. In three trials, raw salmon fillets were surface inoculated with L. monocytogenes, marinated, smoked at 26 to 30 degrees C, and stored at 4 or 10 degrees C for up to 30 days. At dif...

journal_title：Applied and environmental microbiology

authors： Guyer S,Jemmi T

更新日期：1991-05-01 00:00:00

abstract：:Dairy propionic acid bacteria, particularly the species Propionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lys...

journal_title：Applied and environmental microbiology

authors： Hervé C,Coste A,Rouault A,Fraslin JM,Gautier M

更新日期：2001-01-01 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00

abstract：:In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly c...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE

更新日期：1982-03-01 00:00:00

abstract：:Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

journal_title：Applied and environmental microbiology

authors： Bernhard AE,Field KG

更新日期：2000-10-01 00:00:00

abstract：:The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

journal_title：Applied and environmental microbiology

authors： Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

更新日期：2005-09-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:A proteinaceous substance with antibiotic-like activity, resembling that of a bacteriocin, was isolated from an industrial-scale acetone-butanol fermentation of Clostridium acetobutylicum. The substance, purified by acetone precipitation, diethylaminoethyl cellulose chromatography, and polyacrylamide gel electrophores...

journal_title：Applied and environmental microbiology

authors： Webster JR,Reid SJ,Jones DT,Woods DR

更新日期：1981-02-01 00:00:00

abstract：:Anthrax is a fatal infection of humans and livestock that is caused by the gram-positive bacterium Bacillus anthracis. The virulent strains of B. anthracis are encapsulated and toxigenic. In this paper we describe the development of a PCR technique for identifying spores of B. anthracis. Two 20-mer oligonucleotide pri...

journal_title：Applied and environmental microbiology

authors： Reif TC,Johns M,Pillai SD,Carl M

更新日期：1994-05-01 00:00:00

abstract：:A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene patte...

journal_title：Applied and environmental microbiology

authors： Kingombe CI,D'Aoust JY,Huys G,Hofmann L,Rao M,Kwan J

更新日期：2010-01-01 00:00:00

abstract：:Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...

journal_title：Applied and environmental microbiology

authors： Prol García MJ,D'Alvise PW,Gram L

更新日期：2013-09-01 00:00:00

abstract：:Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

journal_title：Applied and environmental microbiology

authors： Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

更新日期：2013-09-01 00:00:00

abstract：:The growing mycelia of Trichoderma reesei Rut-C30 are richly endowed with endoplasmic reticula and a variety of pleomorphic subcellular bodies. Mycelia of the culture growing in presence of avicel pH101 was fractionated in sucrose density gradients, and several morphologically and biochemically distinct fractions were...

journal_title：Applied and environmental microbiology

authors： Glenn M,Ghosh A,Ghosh BK

更新日期：1985-11-01 00:00:00

abstract：:A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed ...

journal_title：Applied and environmental microbiology

authors： Chen YS,Steele JL

更新日期：1998-09-01 00:00:00

abstract：:The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulate...

journal_title：Applied and environmental microbiology

authors： Palenik B,Koke JA

更新日期：1995-09-01 00:00:00

abstract：:Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase)...

journal_title：Applied and environmental microbiology

authors： Johansson B,Christensson C,Hobley T,Hahn-Hägerdal B

更新日期：2001-09-01 00:00:00

abstract：:Previous studies from this laboratory have documented the presence of coliform bacteria emanating from wooden reservoirs containing finished drinking water. Coliforms were identified as Klebsiella pneumoniae and Enterobacter spp. In the present report, evidence is presented which suggests that the origin of these coli...

journal_title：Applied and environmental microbiology

authors： Bagley ST,Seidler RJ,Talbot HW Jr,Morrow JE

更新日期：1978-07-01 00:00:00

abstract：:We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-lab...

journal_title：Applied and environmental microbiology

authors： Tani K,Kurokawa K,Nasu M

更新日期：1998-04-01 00:00:00

abstract：:Flocs consisting of Anabaena and Zoogloea spp. were used as a model system for the study of planktonic phototroph-heterotroph interactions. In CO(2)-limited continuous culture (3.2 mumol of NaHCO(3) liter h, 1.5 mumol of glucose liter h, pH 8.5, D = 0.026 h), the biomass of the phototroph increased 8.6-fold due to ass...

journal_title：Applied and environmental microbiology

authors： Schiefer GE,Caldwell DE

更新日期：1982-07-01 00:00:00

abstract：:Listeria monocytogenes can change its cellular morphology from bacilli to cocci during the transition to the long-term-survival (LTS) phase. The LTS cells demonstrated increased baro- and thermotolerance compared to their vegetative counterparts. So far, the underlying mechanisms that trigger this morphological and ph...

journal_title：Applied and environmental microbiology

authors： Wen J,Deng X,Li Z,Dudley EG,Anantheswaran RC,Knabel SJ,Zhang W

更新日期：2011-09-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:Recent isolation of Pseudomonas aeruginosa strains from the open ocean and subsequent pulsed-field gel electrophoresis analyses indicate that these strains have a unique genotype (N. H. Khan, Y. Ishii, N. Kimata-Kino, H. Esaki, T. Nishino, M. Nishimura, and K. Kogure, Microb. Ecol. 53:173-186, 2007). We hypothesized t...

journal_title：Applied and environmental microbiology

authors： Khan NH,Ahsan M,Yoshizawa S,Hosoya S,Yokota A,Kogure K

更新日期：2008-10-01 00:00:00

abstract：:Under anoxic conditions, most methoxylated mononuclear aromatic compounds are degraded by bacteria, with catechol being formed as an important intermediate. On the basis of our experiments with the sulfate-reducing bacterium Desulfobacterium sp. strain Cat2, we describe for the first time the enzymatic activities invo...

journal_title：Applied and environmental microbiology

authors： Gorny N,Schink B

更新日期：1994-09-01 00:00:00

abstract：:Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a st...

journal_title：Applied and environmental microbiology

authors： Caruso C,Rizzo C,Mangano S,Poli A,Di Donato P,Finore I,Nicolaus B,Di Marco G,Michaud L,Lo Giudice A

更新日期：2018-01-31 00:00:00

abstract：:Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

journal_title：Applied and environmental microbiology

authors： Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

更新日期：2018-10-30 00:00:00