Abstract:
:Anthrax is a fatal infection of humans and livestock that is caused by the gram-positive bacterium Bacillus anthracis. The virulent strains of B. anthracis are encapsulated and toxigenic. In this paper we describe the development of a PCR technique for identifying spores of B. anthracis. Two 20-mer oligonucleotide primers specific for the capB region of 60-MDa plasmid pXO2 were used for amplification. The amplification products were detected by using biotin- and fluorescein-labeled probes in a novel dual-probe hybridization format. Using the combination of PCR amplification and dual-probe hybridization, we detected two copies of the bacterial genome. Because the PCR assay could detect a minimum of 100 unprocessed spores per PCR mixture, we attempted to facilitate the release of DNA by comparing the effect of limited spore germination with the effect of mechanical spore disruption prior to PCR amplification. The two methods were equally effective and allowed us to identify single spores of B. anthracis in PCR mixtures.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Reif TC,Johns M,Pillai SD,Carl Mdoi
10.1128/AEM.60.5.1622-1625.1994subject
Has Abstractpub_date
1994-05-01 00:00:00pages
1622-5issue
5eissn
0099-2240issn
1098-5336journal_volume
60pub_type
杂志文章abstract::DNA microarray analyses revealed that clusters of repetitive extragenic palindromic PCR-related Escherichia coli isolates were isogenic only within interstitial Lake Huron beach water samples and not in surrounding waters. This suggested that adaptation and growth occurred within the interstitial water sites tested. A...
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.7.2343-2349.1994
更新日期:1994-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1998-10-01 00:00:00
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更新日期:1988-05-01 00:00:00
abstract::[This corrects the article on p. 1743 in vol. 64, PMID: 9572946.]. ...
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更新日期:1998-11-01 00:00:00
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更新日期:1994-09-01 00:00:00
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更新日期:2000-08-01 00:00:00
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