Cloning and sequencing of inulinase and β-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes.

Abstract:

:An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.

journal_name

Appl Environ Microbiol

authors

Kobayashi T,Uchimura K,Deguchi S,Horikoshi K

doi

10.1128/AEM.07442-11

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

2493-5

issue

7

eissn

0099-2240

issn

1098-5336

pii

AEM.07442-11

journal_volume

78

pub_type

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