Nutritional and biochemical characterization of Methanospirillum hungatii.

abstract：:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose cont...

journal_title：Applied and environmental microbiology

authors： Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

更新日期：2011-08-01 00:00:00

abstract：:Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...

journal_title：Applied and environmental microbiology

authors： Cohen-Rosenzweig C,Guan Z,Shaanan B,Eichler J

更新日期：2014-01-01 00:00:00

abstract：:The strain Burkholderia cepacia G4 aerobically mineralized trichloroethene (TCE) to CO(2) over a time period of approximately 20 h. Three biodegradation experiments were conducted with different bacterial optical densities at 540 nm (OD(540)s) in order to test whether isotope fractionation was consistent. The resultin...

journal_title：Applied and environmental microbiology

authors： Barth JA,Slater G,Schüth C,Bill M,Downey A,Larkin M,Kalin RM

更新日期：2002-04-01 00:00:00

abstract：:Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis s...

journal_title：Applied and environmental microbiology

authors： Aronson JN,Arvidson HC

更新日期：1987-02-01 00:00:00

abstract：:To elevate the production level of heterologous polyketide in Streptomyces venezuelae, an additional copy of the positive regulatory gene pikD was introduced into the pikromycin (Pik) polyketide synthase (PKS) deletion mutant of S. venezuelae ATCC 15439 expressing tylosin PKS genes. The resulting mutant strain showed ...

journal_title：Applied and environmental microbiology

authors： Jung WS,Jeong SJ,Park SR,Choi CY,Park BC,Park JW,Yoon YJ

更新日期：2008-04-01 00:00:00

abstract：:Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

journal_title：Applied and environmental microbiology

authors： Rhodes AN,Fulthorpe RR,Tiedje JM

更新日期：2013-11-01 00:00:00

abstract：:The influence of substrate and enzyme concentrations on the rate of saccharification of two defined insoluble cellulose substrates, Avicel (FMC Corp., Philadelphia, Pa.) and Solka-Floc (James River Co., Berlin, N.H.), by the cellulase enzyme system of Trichoderma viride was evaluated. In the assays, enzyme concentrati...

journal_title：Applied and environmental microbiology

authors： Liaw ET,Penner MH

更新日期：1990-08-01 00:00:00

abstract：:The formation of the important flavor compound 4-hydroxy-2,5-dimethyl-3[2H]-furanone (HDMF; Furaneol) from D-fructose-1,6-bisphosphate by the yeast Zygosaccharomyces rouxii was studied with regard to the identification of intermediates present in the culture medium. Addition of o-phenylenediamine, a trapping reagent f...

journal_title：Applied and environmental microbiology

authors： Hauck T,Brühlmann F,Schwab W

更新日期：2003-07-01 00:00:00

abstract：:"Candidatus Nitrosotenuis uzonensis" is the only cultured moderately thermophilic member of the thaumarchaeotal order Nitrosopumilales (NP) that contains many mesophilic marine strains. We examined its membrane lipid composition at different growth temperatures (37°C, 46°C, and 50°C). Its lipids were all membrane-span...

journal_title：Applied and environmental microbiology

authors： Bale NJ,Palatinszky M,Rijpstra WIC,Herbold CW,Wagner M,Sinninghe Damsté JS

更新日期：2019-10-01 00:00:00

abstract：:Hydrolytic extracellular enzymes degrading host tissues potentially play a role in bacterial pathogenesis. Flavobacterium psychrophilum is an important bacterial pathogen of salmonid fish reared in freshwater throughout the world. Diversity among isolates has been described at the phenotypic, serological, and genomic ...

journal_title：Applied and environmental microbiology

authors： Rochat T,Pérez-Pascual D,Nilsen H,Carpentier M,Bridel S,Bernardet JF,Duchaud E

更新日期：2019-03-06 00:00:00

abstract：:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Kohler HP,van der Maarel MJ,Kohler-Staub D

更新日期：1993-03-01 00:00:00

abstract：:A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

journal_title：Applied and environmental microbiology

authors： Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

更新日期：1976-09-01 00:00:00

abstract：:The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

journal_title：Applied and environmental microbiology

authors： Rump LV,Fischer M,González-Escalona N

更新日期：2011-07-01 00:00:00

abstract：:Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from sta...

journal_title：Applied and environmental microbiology

authors： Brown GS,Betty RG,Brockmann JE,Lucero DA,Souza CA,Walsh KS,Boucher RM,Tezak M,Wilson MC,Rudolph T

更新日期：2007-02-01 00:00:00

abstract：:Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp. However,...

journal_title：Applied and environmental microbiology

authors： Lu L,Zeng G,Fan C,Zhang J,Chen A,Chen M,Jiang M,Yuan Y,Wu H,Lai M,He Y

更新日期：2014-06-01 00:00:00

abstract：:The haloalkane dehalogenase enzyme DmmA was identified by marine metagenomic screening. Determination of its crystal structure revealed an unusually large active site compared to those of previously characterized haloalkane dehalogenases. Here we present a biochemical characterization of this interesting enzyme with e...

journal_title：Applied and environmental microbiology

authors： Buryska T,Babkova P,Vavra O,Damborsky J,Prokop Z

更新日期：2018-01-02 00:00:00

abstract：:Fully grown broth cultures of various fast- and slow-growing rhizobia were deliberately diluted with various diluents before their aseptic incorporation into autoclaved peat in polypropylene bags (aseptic method) or mixed with the peat autoclaved in trays (tray method). In a factorial experiment with the aseptic metho...

journal_title：Applied and environmental microbiology

authors： Somasegaran P

更新日期：1985-08-01 00:00:00

abstract：:A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...

journal_title：Applied and environmental microbiology

authors： Paster BJ,Canale-Parola E

更新日期：1985-08-01 00:00:00

abstract：:We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 microM). De...

journal_title：Applied and environmental microbiology

authors： Massieux B,Boivin ME,Van Den Ende FP,Langenskiöld J,Marvan P,Barranguet C,Admiraal W,Laanbroek HJ,Zwart G

更新日期：2004-08-01 00:00:00

abstract：:In this study, we examine bacterial attachment and survival on a titanium (Ti) cathode coated with various carbon nanomaterials (CNM): pristine carbon nanotubes (CNT), oxidized carbon nanotubes (O-CNT), oxidized-annealed carbon nanotubes (OA-CNT), carbon black (CB), and reduced graphene oxide (rGO). The carbon nanomat...

journal_title：Applied and environmental microbiology

authors： Zhang Q,Nghiem J,Silverberg GJ,Vecitis CD

更新日期：2015-07-01 00:00:00

abstract：:Rhodococcus erythropolis HL 24-1 isolated as a 2,4-dinitrophenol-degrading organism can utilize 2-chloro-4,6-dinitrophenol as the sole nitrogen, carbon, and energy source under aerobic conditions. This compound is metabolized with liberation of stoichiometric amounts of chloride and nitrite. Under anaerobic conditions...

journal_title：Applied and environmental microbiology

authors： Lenke H,Knackmuss H

更新日期：1996-03-01 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:Hot-water extracts of mesquite (Prosopis glandulosa) wood were assayed for their total carbohydrate, reducing sugar, and glucose content. These hydrolysates were then used as complete media for yeast growth. A total of 10 strains of yeasts were evaluated for their biomass production in the mesquite wood hydrolysates. ...

journal_title：Applied and environmental microbiology

authors： Stanlake GJ

更新日期：1986-08-01 00:00:00

abstract：:Rhizomucor miehei lipase (RML), as a kind of eukaryotic protein catalyst, plays an important role in the food, organic chemical, and biofuel industries. However, RML retains its catalytic activity below 50°C, which limits its industrial applications at higher temperatures. Soluble expression of this eukaryotic protein...

journal_title：Applied and environmental microbiology

authors： Li G,Fang X,Su F,Chen Y,Xu L,Yan Y

更新日期：2018-01-02 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00

abstract：:In growing cells of Saccharomyces cerevisiae and Saccharomyces carlsbergensis, T-2 toxin inhibits cell growth. We have examined the role of the yeast membranes in the uptake mechanism(s) of T-2 toxin. The effects of membrane-modulating agents, ethanol, cetyltrimethylammonium bromide, Triton X-100, and heat were studie...

journal_title：Applied and environmental microbiology

authors： Schappert KT,Khachatourians GG

更新日期：1984-04-01 00:00:00

abstract：:In this study, we describe the association of three Campylobacter jejuni metabolism-related traits, γ-glutamyl-transpeptidase (GGT), fucose permease (fucP), and secreted L-asparaginase [ansB(s)], with multilocus sequence types (STs). A total of 710 C. jejuni isolates with known STs were selected and originated from hu...

journal_title：Applied and environmental microbiology

authors： de Haan CP,Llarena AK,Revez J,Hänninen ML

更新日期：2012-08-01 00:00:00

abstract：:The activity of glutaraldehyde (GTA) at low concentrations (less than 2%) against poliovirus was assessed by a suspension procedure. The inactivation kinetics showed that concentrations of less than or equal to 0.10% were effective against purified poliovirus at pH 7.2; a 1 log10 reduction was obtained in 70 min with ...

journal_title：Applied and environmental microbiology

authors： Bailly JL,Chambon M,Peigue-Lafeuille H,Laveran H,De Champs C,Beytout D

更新日期：1991-04-01 00:00:00

abstract：:Sporulation of Clostridium botulinum 113B in a complex medium supplemented with certain transition metals (Fe, Mn, Cu, or Zn) at 0.01 to 1.0 mM gave spores that were increased two to sevenfold in their contents of the added metals. The contents of calcium, magnesium, and other metals in the purified spores were relati...

journal_title：Applied and environmental microbiology

authors： Kihm DJ,Hutton MT,Hanlin JH,Johnson EA

更新日期：1990-03-01 00:00:00

abstract：:Sigma B (sigma(B)) is a stress-responsive alternative sigma factor that has been identified in various gram-positive bacteria. Seven different regulators of sigma B (Rsbs) are located in the sigB operons of both Bacillus subtilis and Listeria monocytogenes. In B. subtilis, these proteins contribute to regulation of si...

journal_title：Applied and environmental microbiology

authors： Chaturongakul S,Boor KJ

更新日期：2004-09-01 00:00:00