High-temperature enzymatic breakdown of cellulose.

Abstract:

:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose contacts. Cellulases and glucosidases act by hydrolyzing glycosidic bonds of linear glucose β-1,4-linked polymers, producing glucose. Here we describe eight high-temperature-operating cellulases (TCel enzymes) identified from a survey of thermobacterial and archaeal genomes. Three TCel enzymes preferentially hydrolyzed soluble cellulose, while two preferred insoluble cellulose such as cotton linters and filter paper. TCel enzymes had temperature optima ranging from 85°C to 102°C. TCel enzymes were stable, retaining 80% of initial activity after 120 h at 85°C. Two modes of cellulose breakdown, i.e., with endo- and exo-acting glucanases, were detected, and with two-enzyme combinations at 85°C, synergistic cellulase activity was observed for some enzyme combinations.

journal_name

Appl Environ Microbiol

authors

Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

doi

10.1128/AEM.00199-11

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

5199-206

issue

15

eissn

0099-2240

issn

1098-5336

pii

AEM.00199-11

journal_volume

77

pub_type

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