Reliable and rapid identification of Listeria monocytogenes and Listeria species by artificial neural network-based Fourier transform infrared spectroscopy.

abstract：:Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, sep...

journal_title：Applied and environmental microbiology

authors： Lehman RM,O'Connell SP

更新日期：2002-04-01 00:00:00

abstract：:Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the ac...

journal_title：Applied and environmental microbiology

authors： Yoshida N,Takahashi N,Hiraishi A

更新日期：2005-08-01 00:00:00

abstract：:We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

更新日期：2005-04-01 00:00:00

abstract：:Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhi...

journal_title：Applied and environmental microbiology

authors： Fan Z,Wagschal K,Chen W,Montross MD,Lee CC,Yuan L

更新日期：2009-03-01 00:00:00

abstract：:Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

journal_title：Applied and environmental microbiology

authors： Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

更新日期：2020-06-17 00:00:00

abstract：:A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation t...

journal_title：Applied and environmental microbiology

authors： Jung SC,Smith CL,Lee KS,Hong ME,Kweon DH,Stephanopoulos G,Jin YS

更新日期：2010-09-01 00:00:00

abstract：:Differences in individual host responses have emerged as an issue regarding the health benefits of probiotics. Here, we applied ribosome engineering (RE) technology, developed in an actinomycete study, to Lacticaseibacillus rhamnosus GG (LGG). RE can effectively enhance microbial potential by using antibiotics to indu...

journal_title：Applied and environmental microbiology

authors： Ishida M,Namai F,Shigemori S,Kajikawa S,Tsukagoshi M,Sato T,Ogita T,Shimosato T

更新日期：2020-10-01 00:00:00

abstract：:The blacklegged tick, Ixodes scapularis, is of significant public health importance as a vector of Borrelia burgdorferi, the agent of Lyme borreliosis. The timing of seasonal activity of each immature I. scapularis life stage relative to the next is critical for the maintenance of B. burgdorferi because larvae must fe...

journal_title：Applied and environmental microbiology

authors： Gatewood AG,Liebman KA,Vourc'h G,Bunikis J,Hamer SA,Cortinas R,Melton F,Cislo P,Kitron U,Tsao J,Barbour AG,Fish D,Diuk-Wasser MA

更新日期：2009-04-01 00:00:00

abstract：:Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log10 of VLPs was depurated after 1...

journal_title：Applied and environmental microbiology

authors： Loisy F,Atmar RL,Le Saux JC,Cohen J,Caprais MP,Pommepuy M,Le Guyader FS

更新日期：2005-10-01 00:00:00

abstract：:The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

journal_title：Applied and environmental microbiology

authors： Jain RK,Dreisbach JH,Spain JC

更新日期：1994-08-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fl...

journal_title：Applied and environmental microbiology

authors： Walczysko P,Kuhlicke U,Knappe S,Cordes C,Neu TR

更新日期：2008-01-01 00:00:00

abstract：:Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

journal_title：Applied and environmental microbiology

authors： Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

更新日期：2019-02-06 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal ...

journal_title：Applied and environmental microbiology

authors： Moore WE,Moore LV,Cato EP,Wilkins TD,Kornegay ET

更新日期：1987-07-01 00:00:00

abstract：:The effects of preexposure of soybean (Glycine max L. Merrill) roots to Rhizobium japonicum strains and subsequent establishment of other strains in the nodules were investigated by using combinations of effective strains (USDA 110 and USDA 138) and effective-ineffective strains (USDA 110 and SM-5). Strain USDA 110 wa...

journal_title：Applied and environmental microbiology

authors： Kosslak RM,Bohlool BB,Dowdle S,Sadowsky MJ

更新日期：1983-10-01 00:00:00

abstract：:Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques, in addition to the measurement of chemical profiles. After PCR-assisted sequence retrieval of the 16S rRNA genes (16S rDNA) from...

journal_title：Applied and environmental microbiology

authors： Zepp Falz K,Holliger C,Grosskopf R,Liesack W,Nozhevnikova AN,Müller B,Wehrli B,Hahn D

更新日期：1999-06-01 00:00:00

abstract：:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

更新日期：2005-12-01 00:00:00

abstract：:Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

journal_title：Applied and environmental microbiology

authors： Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

更新日期：2007-01-01 00:00:00

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell n...

journal_title：Applied and environmental microbiology

authors： Reed DW,Fujita Y,Delwiche ME,Blackwelder DB,Sheridan PP,Uchida T,Colwell FS

更新日期：2002-08-01 00:00:00

abstract：:To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) deco...

journal_title：Applied and environmental microbiology

authors： Pel R,Oldenhuis R,Brand W,Vos A,Gottschal JC,Zwart KB

更新日期：1997-02-01 00:00:00

abstract：:The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

journal_title：Applied and environmental microbiology

authors： Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

更新日期：2005-09-01 00:00:00

abstract：:While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

journal_title：Applied and environmental microbiology

authors： Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

更新日期：2018-03-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effectiv...

journal_title：Applied and environmental microbiology

authors： Lu T,Moxley RA,Zhang W

更新日期：2019-05-16 00:00:00

abstract：:Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

journal_title：Applied and environmental microbiology

authors： Miller LK,Dawes KP

更新日期：1978-06-01 00:00:00

abstract：:The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic...

journal_title：Applied and environmental microbiology

authors： Yomantas YA,Tokmakova IL,Gorshkova NV,Abalakina EG,Kazakova SM,Gak ER,Mashko SV

更新日期：2010-01-01 00:00:00

abstract：:The acid-insoluble metal sulfides FeS2, MoS2, and WS2 are chemically attacked by iron(III) hexahydrate ions, generating thiosulfate, which is oxidized to sulfuric acid. Other metal sulfides are attacked by iron(III) ions and by protons, resulting in the formation of elemental sulfur via intermediary polysulfides. Sulf...

journal_title：Applied and environmental microbiology

authors： Schippers A,Sand W

更新日期：1999-01-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:[This corrects the article on p. 662 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-06-01 00:00:00