Comparison of extracellular enzyme activities and community composition of attached and free-living bacteria in porous medium columns.

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00

abstract：:Crude extracts from 14 species of sulfate-reducing bacteria comprising the genera Desulfovibrio, Desulfotomaculum, Desulfobulbus, and Desulfosarcina and from three species of sulfide-oxidizing bacteria were tested in an enzyme-linked immunosorbent assay with polyclonal antisera to adenosine 5'-phosphosulfate reductase...

journal_title：Applied and environmental microbiology

authors： Odom JM,Jessie K,Knodel E,Emptage M

更新日期：1991-03-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

更新日期：1998-07-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbion...

journal_title：Applied and environmental microbiology

authors： Dykstra HR,Weldon SR,Martinez AJ,White JA,Hopper KR,Heimpel GE,Asplen MK,Oliver KM

更新日期：2014-09-01 00:00:00

abstract：:Pectin is a complex uronic acid-containing polysaccharide typically found in plant cell walls, though forms of pectin are also found in marine diatoms and seagrasses. Genetic loci that target pectin have recently been identified in two phyla of marine bacteria. These loci appear to encode a pectin saccharification pat...

journal_title：Applied and environmental microbiology

authors： Hobbs JK,Hettle AG,Vickers C,Boraston AB

更新日期：2018-12-13 00:00:00

abstract：:Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...

journal_title：Applied and environmental microbiology

authors： Patel UD,Govindarajan P,Dave PJ

更新日期：1989-02-01 00:00:00

abstract：:The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

journal_title：Applied and environmental microbiology

authors： Stahl DA,Lane DJ,Olsen GJ,Pace NR

更新日期：1985-06-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the p...

journal_title：Applied and environmental microbiology

authors： Sumida T,Sueyoshi N,Ito M

更新日期：2002-11-01 00:00:00

abstract：:This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage. Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BK virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from ...

journal_title：Applied and environmental microbiology

authors： Bofill-Mas S,Pina S,Girones R

更新日期：2000-01-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00

abstract：:One fluorescent compound previously named TDP-2 was isolated and purified from a rice culture of Fusarium equiseti (Alaska 2-2). Mass spectral and nuclear magnetic resonance data indicated that it is a C-3'-N-acetyl derivative of fusarochromanone, a newly discovered mycotoxin. Time course studies of synthesis of these...

journal_title：Applied and environmental microbiology

authors： Xie WP,Mirocha CJ,Pawlosky RJ,Wen YC,Xu XG

更新日期：1989-04-01 00:00:00

abstract：:Caulobacter crescentus is known to tolerate high levels of uranium [U(VI)], but its detoxification mechanism is poorly understood. Here we show that C. crescentus is able to facilitate U(VI) biomineralization through the formation of U-Pi precipitates via its native alkaline phosphatase activity. The U-Pi precipitates...

journal_title：Applied and environmental microbiology

authors： Yung MC,Jiao Y

更新日期：2014-08-01 00:00:00

abstract：:Male weanling rats fed diets containing fermented and unfermented tannin-free sorghum meals naturally contaminated with traces of ochratoxin A, zearalenone, and an unidentified toxic substance developed anorexia. Mean changes in body weight over a 28-day feeding period for rats fed fermented and unfermented sorghum me...

journal_title：Applied and environmental microbiology

authors： Kazanas N,Ely RW,Fields ML,Erdman JW Jr

更新日期：1984-05-01 00:00:00

abstract：:Environmental contamination with compounds containing oxyanions of chlorine, such as perchlorate or chlorate [(per)chlorate] or chlorine dioxide, has been a constantly growing problem over the last 100 years. Although the fact that microbes reduce these compounds has been recognized for more than 50 years, only six or...

journal_title：Applied and environmental microbiology

authors： Coates JD,Michaelidou U,Bruce RA,O'Connor SM,Crespi JN,Achenbach LA

更新日期：1999-12-01 00:00:00

abstract：:Contamination of soil and water with heavy metals and metalloids is a serious environmental problem. Cadmium and arsenic are major environmental contaminants that pose a serious threat to human health. Although toxicities of cadmium and arsenic to living organisms have been extensively studied, the molecular mechanism...

journal_title：Applied and environmental microbiology

authors： Techo T,Charoenpuntaweesin S,Auesukaree C

更新日期：2020-10-15 00:00:00

abstract：:Soybean trypsin inhibitors are thought to ward off pathogens. Studies with aflatoxigenic strains of Aspergillus flavus and A. parasiticus, frequent soybean contaminants, revealed that trypsin inhibitors do not affect the growth of these fungi and aflatoxin production. Further, the availability of zinc, an essential mi...

journal_title：Applied and environmental microbiology

authors： Stössel P

更新日期：1986-07-01 00:00:00

abstract：:Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

journal_title：Applied and environmental microbiology

authors： Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

更新日期：2019-02-06 00:00:00

abstract：:Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

journal_title：Applied and environmental microbiology

authors： Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

更新日期：2013-03-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid culture...

journal_title：Applied and environmental microbiology

authors： Fang W,Leng B,Xiao Y,Jin K,Ma J,Fan Y,Feng J,Yang X,Zhang Y,Pei Y

更新日期：2005-01-01 00:00:00

abstract：:It has been hypothesized that the environment can influence the composition of the nasal microbiota. However, the direct influence of pig farming on the anterior and posterior nasal microbiota is unknown. Using a cross-sectional design, pig farms (n = 28) were visited in 2014 to 2015, and nasal swabs from 43 pig farme...

journal_title：Applied and environmental microbiology

authors： Kraemer JG,Ramette A,Aebi S,Oppliger A,Hilty M

更新日期：2018-03-01 00:00:00

abstract：:This study describes the optimization of PCR parameters and testing of a wide number of microbial species to establish a highly specific and sensitive PCR-based method of detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 strain in pure cultures and seeded waters from the Gulf of Mexico (gulf water). ...

journal_title：Applied and environmental microbiology

authors： Myers ML,Panicker G,Bej AK

更新日期：2003-04-01 00:00:00

abstract：:Pseudobactin production by Pseudomonas putida WCS358 significantly improves biological control of fusarium wilt caused by nonpathogenic Fusarium oxysporum Fo47b10 (P. Lemanceau, P. A. H. M. Bakker, W. J. de Kogel, C. Alabouvette, and B. Schippers, Appl. Environ. Microbiol. 58:2978-2982, 1992). The antagonistic effect ...

journal_title：Applied and environmental microbiology

authors： Lemanceau P,Bakker PA,De Kogel WJ,Alabouvette C,Schippers B

更新日期：1993-01-01 00:00:00

abstract：:Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...

journal_title：Applied and environmental microbiology

authors： Reeves CD,Hu Z,Reid R,Kealey JT

更新日期：2008-08-01 00:00:00

abstract：:Although tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the ...

journal_title：Applied and environmental microbiology

authors： Beer KD,Wurtmann EJ,Pinel N,Baliga NS

更新日期：2014-03-01 00:00:00

abstract：:Lactic and acetic acids were determined to be slightly synergistic in their inhibitory interrelationship against Salmonella typhimurium with the use of a modified toxicological model. ...

journal_title：Applied and environmental microbiology

authors： Rubin HE

更新日期：1978-10-01 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1omega5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant specie...

journal_title：Applied and environmental microbiology

authors： Olsson PA,van Aarle IM,Gavito ME,Bengtson P,Bengtsson G

更新日期：2005-05-01 00:00:00