Involvement of the Cell Wall Integrity Pathway of Saccharomyces cerevisiae in Protection against Cadmium and Arsenate Stresses.

Abstract:

:Contamination of soil and water with heavy metals and metalloids is a serious environmental problem. Cadmium and arsenic are major environmental contaminants that pose a serious threat to human health. Although toxicities of cadmium and arsenic to living organisms have been extensively studied, the molecular mechanisms of cellular responses to cadmium and arsenic remain poorly understood. In this study, we demonstrate that the cell wall integrity (CWI) pathway is involved in coping with cell wall stresses induced by cadmium and arsenate through its role in the regulation of cell wall modification. Interestingly, the Rlm1p and SBF (Swi4p-Swi6p) complex transcription factors of the CWI pathway were shown to be specifically required for tolerance to cadmium and arsenate, respectively. Furthermore, we found the PIR2 gene, encoding cell wall O-mannosylated heat shock protein, whose expression is under the control of the CWI pathway, is important for maintaining cell wall integrity during cadmium and arsenate stresses. In addition, our results revealed that the CWI pathway is involved in modulating the expression of genes involved in cell wall biosynthesis and cell cycle control in response to cadmium and arsenate via distinct sets of transcriptional regulators.IMPORTANCE Environmental pollution by metal/metalloids such as cadmium and arsenic has become a serious problem in many countries, especially in developing countries. This study shows that in the yeast S. cerevisiae, the CWI pathway plays a protective role against cadmium and arsenate through the upregulation of genes involved in cell wall biosynthesis and cell cycle control, possibly in order to modulate cell wall reconstruction and cell cycle phase transition, respectively. These data provide insights into molecular mechanisms underlying adaptive responses to cadmium and arsenate.

journal_name

Appl Environ Microbiol

authors

Techo T,Charoenpuntaweesin S,Auesukaree C

doi

10.1128/AEM.01339-20

subject

Has Abstract

pub_date

2020-10-15 00:00:00

issue

21

eissn

0099-2240

issn

1098-5336

pii

AEM.01339-20

journal_volume

86

pub_type

杂志文章
  • Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon.

    abstract::A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon in...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4935-4937.2005

    authors: Bouhenni R,Gehrke A,Saffarini D

    更新日期:2005-08-01 00:00:00

  • Chitinase gene sequences retrieved from diverse aquatic habitats reveal environment-specific distributions.

    abstract::Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.6977-6983.2004

    authors: LeCleir GR,Buchan A,Hollibaugh JT

    更新日期:2004-12-01 00:00:00

  • Molybdenum oxidation by Thiobacillus ferrooxidans.

    abstract::Thiobacillus ferrooxidans AP19-3 oxidized molybdenum blue (Mo) enzymatically. Molybdenum oxidase in the plasma membrane of this bacterium was purified ca. 77-fold compared with molybdenum oxidase in cell extract. A purified molybdenum oxidase showed characteristic absorption maxima due to reduced-type cytochrome oxida...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1768-1771.1992

    authors: Sugio T,Hirayama K,Inagaki K,Tanaka H,Tano T

    更新日期:1992-05-01 00:00:00

  • Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum.

    abstract::Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with dif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02004-09

    authors: Muniz Mde M,Morais E Silva Tavares P,Meyer W,Nosanchuk JD,Zancope-Oliveira RM

    更新日期:2010-07-01 00:00:00

  • Alignment of genetic and physical maps of Gibberella zeae.

    abstract::We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01866-07

    authors: Lee J,Jurgenson JE,Leslie JF,Bowden RL

    更新日期:2008-04-01 00:00:00

  • Salt stress proteins induced in Listeria monocytogenes.

    abstract::The ability of Listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. In order to understand the survival mechanisms of L. monocytogenes, an initial approach using two-dimensional polyacrylami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1491-1498.2002

    authors: Duché O,Trémoulet F,Glaser P,Labadie J

    更新日期:2002-04-01 00:00:00

  • Small pectinolytic spirochetes from the rumen.

    abstract::Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.4.919-922.1980

    authors: Ziołecki A,Wojciechowicz M

    更新日期:1980-04-01 00:00:00

  • Effect of UV light disinfection on antibiotic-resistant coliforms in wastewater effluents.

    abstract::Total coliforms and total coliforms resistant to streptomycin, tetracycline, or chloramphenicol were isolated from filtered activated sludge effluents before and after UV light irradiation. Although the UV irradiation effectively disinfected the wastewater effluent, the percentage of the total surviving coliform popul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.371-377.1982

    authors: Meckes MC

    更新日期:1982-02-01 00:00:00

  • Comparison of extracellular enzyme activities and community composition of attached and free-living bacteria in porous medium columns.

    abstract::Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, sep...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1569-1575.2002

    authors: Lehman RM,O'Connell SP

    更新日期:2002-04-01 00:00:00

  • Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols.

    abstract::A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03873-12

    authors: Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

    更新日期:2013-04-01 00:00:00

  • Difluoromethane, a new and improved inhibitor of methanotrophy

    abstract::Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation by Methylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4357-4362.1998

    authors: Miller LG,Sasson C,Oremland RS

    更新日期:1998-11-01 00:00:00

  • Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid.

    abstract::A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for me...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00871-10

    authors: Gallardo O,Fernández-Fernández M,Valls C,Valenzuela SV,Roncero MB,Vidal T,Díaz P,Pastor FI

    更新日期:2010-09-01 00:00:00

  • Genetic divergence of bradyrhizobium strains nodulating soybeans as revealed by multilocus sequence analysis of genes inside and outside the symbiosis island.

    abstract::The genus Bradyrhizobium has been considered to be a taxonomically difficult group. In this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among Bradyrhizobium strains nodulating soybeans in China. Eleven genospecies were identified by sequence analysis of three phyl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00044-14

    authors: Zhang XX,Guo HJ,Wang R,Sui XH,Zhang YM,Wang ET,Tian CF,Chen WX

    更新日期:2014-05-01 00:00:00

  • Effects of the 20-kilodalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis.

    abstract::Bacillus thuringiensis produces large amounts of various pesticidal proteins during the stationary phase. In order to achieve a high yield and form crystals, some pesticidal proteins require the presence of other proteins. Helper protein P20 is required for efficient production of both the Cyt1A and Cry11A crystal pro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.12.5362-5369.2001

    authors: Shao Z,Liu Z,Yu Z

    更新日期:2001-12-01 00:00:00

  • Effects of combinations of substrates on maximum growth rates of several rumen bacteria.

    abstract::Five rumen bacteria, Selenomonas ruminantium, Bacteroides ruminicola, Megasphaera elsdenii, Butyrivibrio fibrisolvens, and Streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. Each bacterium was grown with every substrate that it...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.37.3.544-549.1979

    authors: Russell JB,Delfino FJ,Baldwin RL

    更新日期:1979-03-01 00:00:00

  • Effect of hydrophobicity of utilization of peptides by ruminal bacteria in vitro.

    abstract::When mixed ruminal bacteria were incubated with a pancreatic casein hydrolysate and free amino acids of a similar composition, rates of ammonia production were much greater for peptides than for amino acids. The pancreatic digest of casein was then fractionated with 90% isopropyl alcohol. Hydrophobic peptides which di...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.9.2021-2025.1987

    authors: Chen G,Strobel HJ,Russell JB,Sniffen CJ

    更新日期:1987-09-01 00:00:00

  • Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia.

    abstract::Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3425-3433.2004

    authors: Hewson I,Fuhrman JA

    更新日期:2004-06-01 00:00:00

  • Natural communities of novel archaea and bacteria growing in cold sulfurous springs with a string-of-pearls-like morphology.

    abstract::We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2336-2344.2001

    authors: Rudolph C,Wanner G,Huber R

    更新日期:2001-05-01 00:00:00

  • Characterization of a Small RNA-Containing Virus in Field-Collected Larvae of the Tussock Moth, Lymantria ninayi, from Papua New Guinea.

    abstract::Field-collected larvae of the tussock moth, Lymantria ninayi, a major pest of exotic pines in Papua New Guinea, were found to contain a small RNA-containing virus with a diameter of 29 nm and a buoyant density of 1.32 g/ml. The RNA was single stranded, had a molecular weight of 2.8 x 10, and was polyadenylated. Virion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.3.504-507.1984

    authors: Pullin JS,Black F,King LA,Entwistle PF,Moore NF

    更新日期:1984-09-01 00:00:00

  • Isolation of a Strain of Clostridium thermoaceticum Capable of Growth and Acetic Acid Production at pH 4.5.

    abstract::[This corrects the article on p. 118 in vol. 43.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.4.975-975.1982

    authors:

    更新日期:1982-04-01 00:00:00

  • Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002.

    abstract::Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01751-15

    authors: Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

    更新日期:2015-09-01 00:00:00

  • Modulation of thiol-disulfide oxidoreductases for increased production of disulfide-bond-containing proteins in Bacillus subtilis.

    abstract::Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in bacteria, such as the well-known "cell factory" Bacillus subtilis, it is often the correct formation of disulfide bonds tha...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00894-08

    authors: Kouwen TR,Dubois JY,Freudl R,Quax WJ,van Dijl JM

    更新日期:2008-12-01 00:00:00

  • Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds.

    abstract::A total of 1,794 migrating birds trapped at a coastal site in southern Sweden were sampled for detection of Campylobacter spp. All isolates phenotypically identified as Campylobacter jejuni and a subset of those identified as non-C. jejuni were identified to the species level by PCR-based techniques. C. jejuni was fou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.12.5911-5917.2002

    authors: Waldenström J,Broman T,Carlsson I,Hasselquist D,Achterberg RP,Wagenaar JA,Olsen B

    更新日期:2002-12-01 00:00:00

  • Evaluation of physical coverings used to control Escherichia coli O157:H7 at the compost heap surface.

    abstract::Throughout four field trials, compost heaps covered with finished compost maintained temperatures under the physical covering that were ca. 7 to 15.5°C higher, resulting in rapid Escherichia coli O157:H7 reduction, than those of the heaps covered with fresh straw or left uncovered. Our results validated recommendation...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02940-10

    authors: Shepherd MW Jr,Kim J,Jiang X,Doyle MP,Erickson MC

    更新日期:2011-07-01 00:00:00

  • Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure.

    abstract::Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reducti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.2243-2247.2000

    authors: Woo IS,Rhee IK,Park HD

    更新日期:2000-05-01 00:00:00

  • Temperature-dependent induction of an acid-inducible stimulon of Escherichia coli in broth.

    abstract::The induction of the inducible lysyl-tRNA synthetase, LysU, and the inducible lysine and arginine decarboxylases of Escherichia coli K-12 grown in AC broth to a pH of 5.5 or less is temperature dependent, being distinctly lower at 24 than at 37 degrees C. This induction does not appear to be under HtpR control. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.8.2704-2707.1992

    authors: Hassani M,Pincus DH,Bennett GN,Hirshfield IN

    更新日期:1992-08-01 00:00:00

  • Recovery and phylogenetic analysis of nifH sequences from diazotrophic bacteria associated with dead aboveground biomass of Spartina alterniflora.

    abstract::DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.11.5308-5314.2001

    authors: Lovell CR,Friez MJ,Longshore JW,Bagwell CE

    更新日期:2001-11-01 00:00:00

  • Proline reverses the abnormal phenotypes of Colletotrichum trifolii associated with expression of endogenous constitutively active Ras.

    abstract::Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1647-1651.2002

    authors: Memmott SD,Ha YS,Dickman MB

    更新日期:2002-04-01 00:00:00

  • Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.

    abstract::Selectively regulating gene expression is an essential molecular tool that is lacking for many pathogenic gram-positive bacteria. In this report, we describe the evaluation of a series of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor in Enterococcus faecium, Enterococcus faecalis, and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3385-3392.2003

    authors: Schofield DA,Westwater C,Hoel BD,Werner PA,Norris JS,Schmidt MG

    更新日期:2003-06-01 00:00:00

  • Detection of poliovirus, hepatitis A virus, and rotavirus from sewage and ocean water by triplex reverse transcriptase PCR.

    abstract::A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.7.2400-2407.1994

    authors: Tsai YL,Tran B,Sangermano LR,Palmer CJ

    更新日期:1994-07-01 00:00:00