Utilization of ganglioside-degrading Paenibacillus sp. strain TS12 for production of glucosylceramide.

Abstract:

:Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the production of glucosylceramide with this bacterium. GM1a ganglioside was found to be sequentially degraded by Paenibacillus sp. strain TS12, which was isolated from soil, as follows: GM1a --> asialo GM1 --> asialo GM2 --> lactosylceramide --> glucosylceramide. TS12 was found to produce a series of ganglioside-degrading enzymes, such as sialidases, beta-galactosidases, and beta-hexosaminidases. TS12 also produced beta-glucosidases, but glucosylceramide was somewhat resistant to the bacterial enzyme under the conditions used. Taking advantage of the specificity, we developed a new method for the production of glucosylceramide using TS12 as a biocatalyst. The method involves the conversion of crude bovine brain gangliosides to glucosylceramide by coculture with TS12 and purification of the product by chromatography with Wakogel C-300 HG.

journal_name

Appl Environ Microbiol

authors

Sumida T,Sueyoshi N,Ito M

doi

10.1128/aem.68.11.5241-5248.2002

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

5241-8

issue

11

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

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