Detection of Toxoplasma gondii oocysts in drinking water.

Abstract:

:The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gondii. The method developed was based on the current U.S. Environmental Protection Agency method for detection of Cryptosporidium oocysts. Collection of large-volume drinking water samples and cartridge filter processing were unchanged, although identification of Toxoplasma oocysts in the filter retentate was carried out by using a previously described rodent model. Validation of the method developed was tested by using oocysts from a well-characterized Toxoplasma strain.

journal_name

Appl Environ Microbiol

authors

Isaac-Renton J,Bowie WR,King A,Irwin GS,Ong CS,Fung CP,Shokeir MO,Dubey JP

doi

10.1128/AEM.64.6.2278-2280.1998

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

2278-80

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

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