Abstract:
:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA transcription start sites corresponding to two functional promoters were found, expression from the upstream promoter being autogenously regulated through a catabolite-responsive element (cre) sequence overlapping the upstream +1 site. During growth on ribose, the upstream promoter showed maximal expression, while growth on glucose led to transcription from the downstream promoter. In a ccpA mutant strain, the gene was transcribed mainly from the upstream promoter in both repressing and non repressing conditions. Expression of two enzyme activities, beta-glucosidase and beta-galactosidase, was relieved from carbon catabolite repression in the ccpA mutant strain. In vivo footprinting analysis of the catabolite-controlled bglH gene regulatory region in the ccpA mutant strain showed loss of protection of the cre under repressing conditions.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Muscariello L,Marasco R,De Felice M,Sacco Mdoi
10.1128/AEM.67.7.2903-2907.2001keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
2903-7issue
7eissn
0099-2240issn
1098-5336journal_volume
67pub_type
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