Abstract:
:The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 grew to a maximal density of 3.0 x 10(7) cells per ml after compete reduction of 2.0 mM amaranth in a defined medium. This was accompanied by a stoichiometric consumption of 4.0 mM formate over time when amaranth and formate were supplied as the sole electron acceptor and donor, respectively, suggesting that microbial azoreduction is an electron transport process and that this electron transport can yield energy to support growth. Purified membranous, periplasmic, and cytoplasmic fractions from S12 were analyzed, but only the membranous fraction was capable of reducing azo dyes with formate, lactate, pyruvate, or H(2) as the electron donor. The presence of 5 microM Cu(2+) ions, 200 microM dicumarol, 100 microM stigmatellin, and 100 microM metyrapone inhibited anaerobic azoreduction activity by both whole cells and the purified membrane fraction, showing that dehydrogenases, cytochromes, and menaquinone are essential electron transfer components for azoreduction. These results provide evidence that the microbial anaerobic azoreduction is linked to the electron transport chain and suggest that the dissimilatory azoreduction is a form of microbial anaerobic respiration. These findings not only expand the number of potential electron acceptors known for microbial energy conservation but also elucidate the mechanisms of microbial anaerobic azoreduction.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hong Y,Xu M,Guo J,Xu Z,Chen X,Sun Gdoi
10.1128/AEM.01415-06subject
Has Abstractpub_date
2007-01-01 00:00:00pages
64-72issue
1eissn
0099-2240issn
1098-5336pii
AEM.01415-06journal_volume
73pub_type
杂志文章abstract::Trypticase soy agar supplemented with sucrose, sodium chloride, bile salts, and triphenyltetrazolium chloride is an improved plating medium for the isolation of Vibrio parahaemolyticus from samples of seawater, permitting better differentiation of this organism from Vibrio alginolyticus and other bacteria. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.1.310-312.1983
更新日期:1983-01-01 00:00:00
abstract::The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.10.2486-2491.1988
更新日期:1988-10-01 00:00:00
abstract::Flies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence of Escherichia coli strains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the res...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04252-14
更新日期:2015-06-01 00:00:00
abstract::Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA (PsJN::gusA11) genes w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.1685-1693.2005
更新日期:2005-04-01 00:00:00
abstract::A membrane filter technique has been developed for the enumeration of bifidobacteria in natural aquatic environments. The technique is quantitative, selective, and differential. The medium (YN-6) contains: yeast extract, 2.0 g; agar, 1.5 g; polypeptone peptone, 1.0 g; vitamin-free Casamino Acids, 0.8 g; sodium chlorid...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.3.427-432.1981
更新日期:1981-09-01 00:00:00
abstract::Clostridium-botulinum type A and type B spores were stored in tomato juice (pH 4.2) and citric acid-phosphate buffer (pH 4.2) at 4, 22, and 32 degrees C for 180 days. The spore count was determined at different intervals over the 180-day storage period. There was no significant decrease in the number of type A spores ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.1.30-33.1977
更新日期:1977-07-01 00:00:00
abstract::Representatives of Actinobacteria were isolated from the marine sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00780-10
更新日期:2010-06-01 00:00:00
abstract::Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extrarad...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00421-14
更新日期:2014-07-01 00:00:00
abstract::Olavius crassitunicatus is a small symbiont-bearing worm that occurs at high abundance in oxygen-deficient sediments in the East Pacific Ocean. Using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization, we examined the diversity and phylogeny of bacterial symbionts in two geographically dista...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.3.1553-1561.2005
更新日期:2005-03-01 00:00:00
abstract::The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04146-13
更新日期:2014-04-01 00:00:00
abstract::We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.1.38-42.1998
更新日期:1998-01-01 00:00:00
abstract::Bacteroides xylanolyticus X5-1 was grown in pure culture and in mixed culture with Methanospirillum hungatei JF-1 under xylose limitation in the chemostat. In the pure culture, ethanol, acetate, CO(2), and hydrogen were the products. In the mixed culture, acetate, CO(2), and presumably hydrogen were the only products ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.4.1347-1352.1994
更新日期:1994-04-01 00:00:00
abstract::Given the interest in Bacteroides species as microbial source tracking (MST) markers, and the limited knowledge of the survival of Bacteroides species in the environment, here we examine the survival of Bacteroides fragilis, B. thetaiotaomicron, and environmental species of Bacteroides by use of culture techniques and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00883-10
更新日期:2010-11-01 00:00:00
abstract::DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.6.2402-2407.1995
更新日期:1995-06-01 00:00:00
abstract::Free-living amoebae are frequent hosts for bacterial endosymbionts. In this study, the symbionts of eight novel environmental Acanthamoeba strains isolated from different locations worldwide were characterized. Phylogenetic analysis revealed that they were related to one of four evolutionary lineages of amoeba symbion...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01093-08
更新日期:2008-09-01 00:00:00
abstract::Fungal attacks on stored vegetable and fruits are responsible for losses of products. There is an active research field to develop alternative strategies for postharvest disease management, and the use of biocontrol agents represents a promising approach. Understanding the molecular bases of the biocontrol activity of...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02910-20
更新日期:2021-01-15 00:00:00
abstract::Of 70 pure microbial cultures isolated from aquatic habitats, soil, and air according to the ability to lyse live blue-green algae, 62 were fungi representing the genera Acremonium, Emericellopsis, and Verticillium. Algal-lysing fungi were isolated from all habitat types sampled. The remaining isolates comprised four ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.5.962-969.1978
更新日期:1978-05-01 00:00:00
abstract::The proportion of oocysts of Cryptosporidium parvum showing a fold on oocyst walls when incubated with either fluorescent monoclonal antibody or a surface-reactive fluorescent dye was increased by incubating suspensions of oocysts with dimethyl sulfoxide, sucrose, or Hanks' balanced salt solution. Further incubation o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.8.2638-2641.1993
更新日期:1993-08-01 00:00:00
abstract::Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide n...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02403-15
更新日期:2015-12-01 00:00:00
abstract::Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia coli recombinants. S...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.12.5619-5623.1999
更新日期:1999-12-01 00:00:00
abstract::Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4993-4995.1997
更新日期:1997-12-01 00:00:00
abstract::The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06174-11
更新日期:2011-11-01 00:00:00
abstract::A beta-mannanase (EC 3.2.1.78) from Vibrio sp. strain MA-138 was purified by ammonium sulfate precipitation and several chromatographic procedures including gel filtration, adsorption, and ion-exchange chromatographies. The final ion-exchange chromatography Mono Q yielded one major active fraction and three minor acti...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.12.4454-4458.1995
更新日期:1995-12-01 00:00:00
abstract::The presence of Yersinia ruckeri in a French fish farm was investigated. Y. ruckeri was isolated mainly from algae and sediment samples rather than from water. Twenty-two Y. ruckeri isolates were obtained, and three strains were distinguished by enterobacterial repetitive intergenic consensus PCR amplification. These ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.470-475.2002
更新日期:2002-02-01 00:00:00
abstract::Microscopic eukaryotes are known to have important ecosystem functions, but their diversity in most environments remains vastly unexplored. Here we analyzed an 18S rRNA gene library from a subsurface iron- and sulfur-oxidizing microbial community growing in highly acidic (pH < 0.9) runoff within the Richmond Mine at I...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02500-08
更新日期:2009-04-01 00:00:00
abstract::We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.1.111-119.1994
更新日期:1994-01-01 00:00:00
abstract::We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we la...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00124-08
更新日期:2008-08-01 00:00:00
abstract::Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enterovir...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.2070-2078.2005
更新日期:2005-04-01 00:00:00
abstract::Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01595-08
更新日期:2009-02-01 00:00:00
abstract::Bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of Treponema hyodysenteriae. Isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.5.964-969.1984
更新日期:1984-11-01 00:00:00