Multiplexed integrating plasmids for engineering of the erythromycin gene cluster for expression in Streptomyces spp. and combinatorial biosynthesis.

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Bottlenecks in protein expression and secretion often limit the development of industrial processes. By manipulating chaperone and foldase levels, improvements in yeast secretion were found for a number of proteins. Recently, sustained endoplasmic reticulum stress, occurring due to recombinant protein production, was ...

journal_title：Applied and environmental microbiology

authors： Raimondi S,Zanni E,Talora C,Rossi M,Palleschi C,Uccelletti D

更新日期：2008-12-01 00:00:00

abstract：:Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination o...

journal_title：Applied and environmental microbiology

authors： Langendijk PS,Schut F,Jansen GJ,Raangs GC,Kamphuis GR,Wilkinson MH,Welling GW

更新日期：1995-08-01 00:00:00

abstract：:Marker rescue is an important molecular technique that enables sequential gene deletions. The Cre-loxP recombination system has been used for marker gene rescue in various organisms, including aspergilli. However, this system requires many time-consuming steps, including construction of a Cre expression plasmid, intro...

journal_title：Applied and environmental microbiology

authors： Mizutani O,Masaki K,Gomi K,Iefuji H

更新日期：2012-06-01 00:00:00

abstract：:Lyophilization was used to concentrate bovine feces prior to DNA extraction and analysis using real-time PCR. Lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both Escherichia coli O157:H7 and Campylobacter jejuni bact...

journal_title：Applied and environmental microbiology

authors： Rapp D,Waller J,Brightwell G,Muirhead RW

更新日期：2010-03-01 00:00:00

abstract：:Pyruvate carboxylase (PYC) is an ecologically, medically, and industrially important enzyme. It is widespread in all three domains of life, the archaea, bacteria, and eukarya. PYC catalyzes ATP-dependent carboxylation of pyruvate to oxaloacetate. Detailed structure-function studies of this enzyme have been hampered du...

journal_title：Applied and environmental microbiology

authors： Lai H,Kraszewski JL,Purwantini E,Mukhopadhyay B

更新日期：2006-12-01 00:00:00

abstract：:Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activit...

journal_title：Applied and environmental microbiology

authors： Mayer VW,Goin CJ

更新日期：1983-02-01 00:00:00

abstract：:A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

journal_title：Applied and environmental microbiology

authors： Visscher PT,Taylor BF

更新日期：1994-12-01 00:00:00

abstract：:rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...

journal_title：Applied and environmental microbiology

authors： Parker MA

更新日期：2002-04-01 00:00:00

abstract：:A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

journal_title：Applied and environmental microbiology

authors： Tang M,Bideshi DK,Park HW,Federici BA

更新日期：2006-11-01 00:00:00

abstract：:The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates. This assumption is based solely on early chemical evidence (B. H. Davies, Biochem. J. 116:93-99, 1...

journal_title：Applied and environmental microbiology

authors： Wang GS,Grammel H,Abou-Aisha K,Sägesser R,Ghosh R

更新日期：2012-10-01 00:00:00

abstract：:Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and ...

journal_title：Applied and environmental microbiology

authors： Meunier A,Schmit JF,Stas A,Kutluk N,Bragard C

更新日期：2003-04-01 00:00:00

abstract：:[This corrects the article on p. 711 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-11-01 00:00:00

abstract：:The metabolic conversion of 5-methylfurfural and 2-methylfurfural to furfural by a methanogenic bacterium, Methanococcus sp. strain B, was studied. This bacterium was found to use methylfurfural compounds as a growth substrate and to convert them stoichiometrically to furfural. For every mole of methylfurfurals metabo...

journal_title：Applied and environmental microbiology

authors： Boopathy R

更新日期：1996-09-01 00:00:00

abstract：:In acetic acid fermentation by Acetobacter aceti, the acetic acid produced inhibits the production of acetic acid by this microorganism. To alleviate this inhibitory effect, we developed an electrodialysis fermentation method such that acetic acid is continuously removed from the broth. The fermentation unit has a com...

journal_title：Applied and environmental microbiology

authors： Nomura Y,Iwahara M,Hongo M

更新日期：1988-01-01 00:00:00

abstract：:Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

journal_title：Applied and environmental microbiology

authors： Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

更新日期：2010-04-01 00:00:00

abstract：:The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

journal_title：Applied and environmental microbiology

authors： Wei X,Bauer WD

更新日期：1998-05-01 00:00:00

abstract：:Copper resistance mechanisms provide an important adaptive advantage to plant pathogenic bacteria under exposure to copper treatments. Copper resistance determinants have been described in Pseudomonas syringae pv. syringae (Pss) strains isolated from mango intimately associated with 62 kb plasmids belonging to the pPT...

journal_title：Applied and environmental microbiology

authors： Aprile F,Heredia-Ponce Z,Cazorla FM,de Vicente A,Gutiérrez-Barranquero JA

更新日期：2020-12-23 00:00:00

abstract：:The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

journal_title：Applied and environmental microbiology

authors： McMeekin TA

更新日期：1977-06-01 00:00:00

abstract：:Six strains of a new species, Legionella sainthelensi, were isolated from freshwater in areas affected by the volcanic eruptions of Mt. St. Helens in the state of Washington. Strains of L. sainthelensi are culturally and biochemically similar to other legionellae. They grow on buffered charcoal yeast agar but not on m...

journal_title：Applied and environmental microbiology

authors： Campbell J,Bibb WF,Lambert MA,Eng S,Steigerwalt AG,Allard J,Moss CW,Brenner DJ

更新日期：1984-02-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：UNLABELLED:The McMurdo Dry Valleys (MCM) of southern Victoria Land, Antarctica, harbor numerous ice-covered bodies of water that provide year-round liquid water oases for isolated food webs dominated by the microbial loop. Single-cell microbial eukaryotes (protists) occupy major trophic positions within this truncated ...

journal_title：Applied and environmental microbiology

authors： Li W,Podar M,Morgan-Kiss RM

更新日期：2016-05-31 00:00:00

abstract：:Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

journal_title：Applied and environmental microbiology

authors： Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

更新日期：2020-05-19 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:We developed a new cell surface display system in Pseudomonas putida KT2442 using OprF, an outer membrane protein of Pseudomonas aeruginosa, as an anchoring motif in a C-terminal deletion-fusion strategy. The Pseudomonas fluorescens SIK W1 lipase gene was fused to two different C-terminal truncated OprF genes, and the...

journal_title：Applied and environmental microbiology

authors： Lee SH,Lee SY,Park BC

更新日期：2005-12-01 00:00:00

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:The Tinto River (Huelva, southwestern Spain) is an extreme environment with a rather constant acidic pH along the entire river and a high concentration of heavy metals. The extreme conditions of the Tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex...

journal_title：Applied and environmental microbiology

authors： González-Toril E,Llobet-Brossa E,Casamayor EO,Amann R,Amils R

更新日期：2003-08-01 00:00:00

abstract：:Escherichia coli is a widely utilized indicator of the sanitary quality of bivalve molluscan shellfish sold for human consumption. However, it is now well documented that shellfish that meet the E. coli standards for human consumption may contain human enteric viruses that cause gastroenteritis and hepatitis. In this ...

journal_title：Applied and environmental microbiology

authors： Doré WJ,Henshilwood K,Lees DN

更新日期：2000-04-01 00:00:00

abstract：:Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

journal_title：Applied and environmental microbiology

authors： Caskey WH,Taber WA

更新日期：1981-07-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00