Abstract:
:Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not indicate whether the cysts were viable, these amplifications are a presence/absence test only. In contrast, amplification of heat shock-induced mRNA utilizing the same HSP primers was indicative of viable Giardia cysts. The limit of sensitivity of the presence/absence test was 1 cyst, whereas for the viability test it was 10 cysts. Thus, viable Giardia cysts can be rapidly and specifically detected with great sensitivity through the use of PCR amplifications.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Abbaszadegan M,Huber MS,Gerba CP,Pepper ILdoi
10.1128/AEM.63.1.324-328.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
324-8issue
1eissn
0099-2240issn
1098-5336journal_volume
63pub_type
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