Abstract:
:Biodegradability screening tests of soil commonly measure 14CO2 evolution from radiolabeled test compounds, and glucose has often served as a positive control. When constant amounts of radiolabel were added to soil in combination with increasing amounts of unlabeled substrates, glucose and some related hexoses behaved in an anomalous manner. In contrast to that of formate, benzoate, n-hexadecane, or bis(2-ethylhexyl) phthalate, dilution of glucose radiocarbon with unlabeled glucose increased rather than decreased the rate and extent of 14CO2 evolution. [14C]glucose incorporation into biomass and Vmax values were consistent with the interpretation that application of relatively high concentrations of glucose to soil shifts the balance of the soil microbial community from the autochthonous (humus-degrading) to the zymogeneous (opportunistic) segment. The higher growth and turnover rates that define zymogeneous microorganisms, combined with a lower level of carbon incorporation into their biomass, result in the evolution of disproportionate percentages of 14CO2. When used as positive controls, glucose and related hexoses may raise the expectations for percent 14CO2 evolution to levels that are not realistic for other biodegradable compounds.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Shen J,Bartha Rdoi
10.1128/AEM.62.7.2411-2415.1996subject
Has Abstractpub_date
1996-07-01 00:00:00pages
2411-5issue
7eissn
0099-2240issn
1098-5336journal_volume
62pub_type
杂志文章abstract::The competition between selected or commercial killer strains of type K2 and sensitive commercial strains of Saccharomyces cerevisiae was studied under various conditions in sterile grape juice fermentations. The focus of this study was the effect of yeast inoculation levels and the role of assimilable nitrogen nutrit...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.63.7.2821-2825.1997
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更新日期:1996-12-01 00:00:00
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更新日期:1987-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00