Abstract:
:We designed five Pseudomonas-selective soil extract NAA media containing the selective properties of trimethoprim and sodium lauroyl sarcosine and 0 to 100% of the amount of Casamino Acids used in the classical Pseudomonas-selective Gould's S1 medium. All of the isolates were confirmed to be Pseudomonas by a Pseudomonas-specific OprF antibody and a Pseudomonas-specific PCR targeting 16S ribosomal DNA. The Pseudomonas isolates were characterized by classical physiological tests, repetitive extragenic palindromic-PCR, Fourier transform infrared spectroscopy, and carbon source utilization patterns. Several of these analyses showed that the amount of Casamino Acids significantly influenced the diversity of the recovered Pseudomonas isolates. Furthermore, the data suggested that specific Pseudomonas subpopulations were represented on the nutrient-poor media. The NAA 1:100 medium, containing ca. 15 mg of organic carbon per liter, consistently gave significantly higher Pseudomonas CFU counts than Gould's S1 when tested on four Danish soils. NAA 1:100 may, therefore, be a better medium than Gould's S1 for enumeration and isolation of Pseudomonas from the low-nutrient soil environment.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Aagot N,Nybroe O,Nielsen P,Johnsen Kdoi
10.1128/AEM.67.11.5233-5239.2001keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
5233-9issue
11eissn
0099-2240issn
1098-5336journal_volume
67pub_type
杂志文章abstract::An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.9.2021-2025.1987
更新日期:1987-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03131-13
更新日期:2014-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:2009-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.1.246-251.1989
更新日期:1989-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.45.6.1943-1945.1983
更新日期:1983-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01986-06
更新日期:2007-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2009-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00