Abstract:
:ATP-dependent phosphorylation of [14C]galactose by 11 strains of Streptococcus thermophilus indicated that these organisms possessed the Leloir enzyme, galactokinase (galK). Activities were 10 times higher in fully induced, galactose-fermenting (Gal+) strains than in galactose-nonfermenting (Gal-) strains. Lactose-grown, Gal- cells released free galactose into the medium and were unable to utilize residual galactose or to induce galK above basal levels. Gal+ S. thermophilus 19258 also released galactose into the medium, but when lactose was depleted growth on galactose commenced, and galK increased from 0.025 to 0.22 micromol of galactose phosphorylated per min per mg of protein. When lactose was added to galactose-grown cells of S. thermophilus 19258, galK activity rapidly decreased. These results suggest that galK in Gal+ S. thermophilus is subject to an induction-repression mechanism, but that galK cannot be induced in Gal- strains.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hutkins R,Morris HA,McKay LLdoi
10.1128/AEM.50.4.777-780.1985subject
Has Abstractpub_date
1985-10-01 00:00:00pages
777-80issue
4eissn
0099-2240issn
1098-5336journal_volume
50pub_type
杂志文章abstract::Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize tr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02922-10
更新日期:2011-06-01 00:00:00
abstract::Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.9.4128-4130.2000
更新日期:2000-09-01 00:00:00
abstract::The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03999-14
更新日期:2015-04-01 00:00:00
abstract::Significant amounts of ethylene were produced by Pseudomonas syringae pv. glycinea, pv. phaseolicola (which had been isolated from viny weed Pueraria lobata [Willd.] Ohwi [common name, kudzu]), and pv. pisi in synthetic medium. On the other hand, the bean strains of P. syringae pv. phaseolicola and strains of 17 other...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.1.156-161.1997
更新日期:1997-01-01 00:00:00
abstract::Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01436-13
更新日期:2013-09-01 00:00:00
abstract::Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00297-06
更新日期:2006-07-01 00:00:00
abstract::The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.12.8920-8924.2005
更新日期:2005-12-01 00:00:00
abstract::Specific oxygen uptake rates [Q(02)] were highest when Candida 107 and Rhodotorula gracilis grew at their maximum dilution rates. In nitrogen-limited medium, which encourages lipid accumulation, Q(02) values of both yeasts decreased as the dilution rate was lowered. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.2.230-231.1977
更新日期:1977-08-01 00:00:00
abstract::The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02188-09
更新日期:2010-11-01 00:00:00
abstract::The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00280-20
更新日期:2020-05-05 00:00:00
abstract::The effects of long-term heavy metal deposition on microbial community structure and the level of bacterial community tolerance were studied along two different gradients in Scandinavian coniferous forest soils. One was near the Harjavalta smelter in Finland, and one was at Ronnskar in Sweden. Phospholipid fatty acid ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.2.420-428.1996
更新日期:1996-02-01 00:00:00
abstract::The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.9.3150-3159.1994
更新日期:1994-09-01 00:00:00
abstract::H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turno...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01959-12
更新日期:2012-12-01 00:00:00
abstract::The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.7.3614-3621.2002
更新日期:2002-07-01 00:00:00
abstract::A bacterium capable of anaerobic growth via reductive dehalogenation of 2-chlorophenol was isolated from a culture enriched from sediment taken from a small stream near Lansing, Mich. The organism, designated strain 2CP-1, is a gram-negative rod ca. 3 by 0.5 micron in size and is a catalase-negative, oxidase-negative,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.10.3536-3542.1994
更新日期:1994-10-01 00:00:00
abstract::The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00379-20
更新日期:2020-07-02 00:00:00
abstract::The kinetics of adhesion of Vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. A modified Langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (Vibrio alginolyticus DP1HE4 and Vibrio anguillarum-like DC12R8 and DC12R9) showed a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.10.3650-3654.1996
更新日期:1996-10-01 00:00:00
abstract::The viability of starved or dying Saccharomyces cerevisiae Y5 cells was extended for up to 1,500 h by adding osmotic supports (0.6 M sorbitol or mannitol) to the starvation media. Replacement of these polyols with 0.3 M KCl, 0.5 M MgSO4, or 0.2 M MgCl2 postponed the death of cells incubated in N-free medium, but accel...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.3.713-716.1985
更新日期:1985-09-01 00:00:00
abstract::Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.3.545-549.1984
更新日期:1984-09-01 00:00:00
abstract::Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00038-06
更新日期:2006-07-01 00:00:00
abstract::As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein of Malassezia furfur in the presence of shampoo. We found that phage display of llama single-domain antibody fragments ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.1.442-450.2005
更新日期:2005-01-01 00:00:00
abstract::Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.12.5259-5266.2000
更新日期:2000-12-01 00:00:00
abstract::In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02032-15
更新日期:2015-10-01 00:00:00
abstract::Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.1.255-257.1982
更新日期:1982-07-01 00:00:00
abstract::Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02609-15
更新日期:2015-11-13 00:00:00
abstract::The minimal nutritional growth requirements were determined for strains Okra B and Iwanai E, which are representatives of groups I and II, respectively, of Clostridium botulinum. These type B and E strains differed considerably in their nutrient requirements. The organic growth factors required in high concentrations ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.3.753-759.1988
更新日期:1988-03-01 00:00:00
abstract::Symbiotic microorganisms are widespread in nature and can play a major role in the ecology and evolution of animals. The aphid-Serratia symbiotica bacterium interaction provides a valuable model to study the mechanisms behind these symbiotic associations. The recent discovery of cultivable S. symbiotica strains with a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02445-18
更新日期:2019-05-02 00:00:00
abstract::Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.2.369-371.1980
更新日期:1980-02-01 00:00:00
abstract::In the vast number of random mutagenesis experiments that have targeted protein thermostability, single amino acid substitutions that increase the apparent melting temperature (Tm) of the enzyme more than 1 to 2 degrees C are rare and often require the creation of a large library of mutated genes. Here we present a ca...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02220-07
更新日期:2008-02-01 00:00:00
abstract::The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.7.2691-2696.1998
更新日期:1998-07-01 00:00:00