Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.

Abstract:

:The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.

journal_name

Appl Environ Microbiol

authors

Llobet-Brossa E,Rosselló-Mora R,Amann R

doi

10.1128/AEM.64.7.2691-2696.1998

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

2691-6

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

64

pub_type

杂志文章
  • Relationship between bacterioplankton richness, respiration, and production in the Southern North Sea.

    abstract::We investigated the relationship between bacterioplankton production (BP), respiration (BR), and community composition measured by terminal restriction fragment length polymorphism in the southern North Sea over a seasonal cycle. Major changes in bacterioplankton richness were apparent from April to December. While ce...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.5.2260-2266.2005

    authors: Reinthaler T,Winter C,Herndl GJ

    更新日期:2005-05-01 00:00:00

  • Regulation of the alginate biosynthesis gene algC in Pseudomonas aeruginosa during biofilm development in continuous culture.

    abstract::Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.3.860-867.1995

    authors: Davies DG,Geesey GG

    更新日期:1995-03-01 00:00:00

  • Inhibition of plant-pathogenic bacteria by short synthetic cecropin A-melittin hybrid peptides.

    abstract::Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.5.3302-3308.2006

    authors: Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

    更新日期:2006-05-01 00:00:00

  • Optimum pH levels for eluting enteroviruses from sludge solids with beef extract.

    abstract::This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1880-1881.1988

    authors: Berg G,Sullivan G

    更新日期:1988-07-01 00:00:00

  • Effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns.

    abstract::Columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. The saturated hydraulic conductivity (HCsat) of the sand was monitored over time. Among the four bacterial strains ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1690-1698.1992

    authors: Vandevivere P,Baveye P

    更新日期:1992-05-01 00:00:00

  • Competition Among Rhizobium spp. for Nodulation of Leucaena leucocephala in Two Tropical Soils.

    abstract::The successful nodulation of legumes by a Rhizobium strain is determined by the competitive ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.5-9.1984

    authors: Moawad H,Bohlool BB

    更新日期:1984-07-01 00:00:00

  • Nonphotosynthetic pigmented bacteria in a potable water treatment and distribution system.

    abstract::The occurrence of pigmented bacteria in potable water, from raw source water through treatment to distribution water, including dead-end locations, was compared at sample sites in a large municipal water system. Media used to enumerate heterotrophic bacteria and differentiate pigmented colonies were standard method pl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.4.912-921.1989

    authors: Reasoner DJ,Blannon JC,Geldreich EE,Barnick J

    更新日期:1989-04-01 00:00:00

  • Chelating Properties of Extracellular Polysaccharides from Chlorella spp.

    abstract::Chlorella stigmatophora LB 993 was grown in artificial seawater under controlled conditions. The production of cell wall polysaccharides attached to the cells and dissolved in the growth medium was monitored during algal growth. Preliminary characterization of the dissolved polysaccharides of C. stigmatophora and othe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2953-2956.1987

    authors: Kaplan D,Christiaen D,Arad SM

    更新日期:1987-12-01 00:00:00

  • Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

    abstract::Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04028-12

    authors: Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

    更新日期:2013-05-01 00:00:00

  • Degradation of 4-Chlorobenzoic Acid by Arthrobacter sp.

    abstract::A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.5.1020-1025.1984

    authors: Marks TS,Smith AR,Quirk AV

    更新日期:1984-11-01 00:00:00

  • Production of ochratoxin A in barley by Aspergillus ochraceus and Penicillium viridicatum: effect of fungal growth, time, temperature, and inoculum size.

    abstract::Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also foll...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.5.1205-1207.1982

    authors: Häggblom P

    更新日期:1982-05-01 00:00:00

  • Enhanced Production of Surfactin from Bacillus subtilis by Continuous Product Removal and Metal Cation Additions.

    abstract::The lipopeptide, surfactin, is produced by Bacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.3.408-412.1981

    authors: Cooper DG,Macdonald CR,Duff SJ,Kosaric N

    更新日期:1981-09-01 00:00:00

  • Ecological Consequences of Long-Term Exposure of Anabaena variabilis (Cyanophyceae) to Shifts in Environmental Factors.

    abstract::Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.1.141-148.1982

    authors: Collins CD,Boylen CW

    更新日期:1982-07-01 00:00:00

  • Biotransformation of linoleic acid and bile acids by Eubacterium lentum.

    abstract::Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. M...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.1.39-43.1984

    authors: Eyssen H,Verhulst A

    更新日期:1984-01-01 00:00:00

  • Isolation and characterization of a novel toluene-degrading, sulfate-reducing bacterium.

    abstract::A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.4.1188-1196.1996

    authors: Beller HR,Spormann AM,Sharma PK,Cole JR,Reinhard M

    更新日期:1996-04-01 00:00:00

  • Enhancement of Exopolysaccharide Production by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772 with a Simplified Defined Medium.

    abstract::The aim of this work was to investigate the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772. The strain was grown in batch cultures on a chemically defined medium, and the technique of single omission of medium components was applied to deter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.4.1333-1337.1998

    authors: Grobben GJ,Chin-Joe I,Kitzen VA,Boels IC,Boer F,Sikkema J,Smith MR,de Bont JA

    更新日期:1998-04-01 00:00:00

  • Bacterial and archaeal diversity in sediments of west Lake Bonney, McMurdo Dry Valleys, Antarctica.

    abstract::Bacterial and archaeal diversity was examined in a sediment core from Lake Bonney, Antarctica. Members of the Archaea showed both low abundance and diversity, whereas bacterial diversity was moderately high and some phyla were fairly abundant, even in geologically old samples. Microbial diversity correlated with sampl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02336-12

    authors: Tang C,Madigan MT,Lanoil B

    更新日期:2013-02-01 00:00:00

  • Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection.

    abstract::Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02327-17

    authors: Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

    更新日期:2018-02-14 00:00:00

  • Thiobacillus cuprinus sp. nov., a Novel Facultatively Organotrophic Metal-Mobilizing Bacterium.

    abstract::Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.2.315-322.1990

    authors: Huber H,Stetter KO

    更新日期:1990-02-01 00:00:00

  • Relative Abundance of Nitrotoga spp. in a Biofilter of a Cold-Freshwater Aquaculture Plant Appears To Be Stimulated by Slightly Acidic pH.

    abstract::The functioning of recirculation aquaculture systems (RAS) is essential to maintain water quality for fish health, and one crucial process here is nitrification. The investigated RAS was connected to a rainbow trout production system and operated at an average temperature of 13°C and pH 6.8. Community analyses of the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03163-15

    authors: Hüpeden J,Wegen S,Off S,Lücker S,Bedarf Y,Daims H,Kühn C,Spieck E

    更新日期:2016-01-08 00:00:00

  • Characterization of the biosynthetic operon for the antibacterial peptide herbicolin in Pantoea vagans biocontrol strain C9-1 and incidence in Pantoea species.

    abstract::Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07351-11

    authors: Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

    更新日期:2012-06-01 00:00:00

  • Production and characterization of antibodies against microcystins.

    abstract::Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.1928-1933.1989

    authors: Chu FS,Huang X,Wei RD,Carmichael WW

    更新日期:1989-08-01 00:00:00

  • Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic.

    abstract::Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1210-1214.2005

    authors: Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

    更新日期:2005-03-01 00:00:00

  • Use of a marker organism to model the spread of central nervous system tissue in cattle and the abattoir environment during commercial stunning and carcass dressing.

    abstract::Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.791-798.2002

    authors: Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

    更新日期:2002-02-01 00:00:00

  • Effect of aerosolization on culturability and viability of gram-negative bacteria.

    abstract::Estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. However, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3585-3588.1997

    authors: Heidelberg JF,Shahamat M,Levin M,Rahman I,Stelma G,Grim C,Colwell RR

    更新日期:1997-09-01 00:00:00

  • Lignin Degradation and Modification by the Soil-Inhabiting Fungus Fusarium proliferatum.

    abstract::A soil-inhabiting Fusarium proliferatum strain was capable of transforming or degrading nonlabeled and (sup14)C-labeled industrial, natural, and synthetic lignin. The mineralization rate per day (expressed as the percentage of added radioactivity recovered as to (sup14)CO(inf2)) was maximal during primary metabolism. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3716-3718.1997

    authors: Regalado V,Rodriguez A,Perestelo F,Carnicero A,De La Fuente G,Falcon MA

    更新日期:1997-09-01 00:00:00

  • Pathogenic Aeromonas hydrophila serogroup O:14 and O:81 strains with an S layer.

    abstract::Five autoagglutinating Aeromonas hydrophila isolates recovered from eels and humans were assigned to serogroups O:14 and O:81 of the Sakazaki and Shimada (National Institutes of Health) scheme. They had the following properties in common: positive precipitation after boiling, moderate surface hydrophobicity (salt-aggr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.5898-5904.2004

    authors: Esteve C,Alcaide E,Canals R,Merino S,Blasco D,Figueras MJ,Tomás JM

    更新日期:2004-10-01 00:00:00

  • High-rate anaerobic treatment of wastewater at low temperatures.

    abstract::Anaerobic treatment of a volatile fatty acid (VFA) mixture was investigated under psychrophilic (3 to 8 degrees C) conditions in two laboratory-scale expanded granular sludge bed reactor stages in series. The reactor system was seeded with mesophilic methanogenic granular sludge and fed with a mixture of VFAs. Good re...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.4.1696-1702.1999

    authors: Lettinga G,Rebac S,Parshina S,Nozhevnikova A,van Lier JB,Stams AJ

    更新日期:1999-04-01 00:00:00

  • Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.

    abstract::The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04146-13

    authors: Hawkins AB,Adams MW,Kelly RM

    更新日期:2014-04-01 00:00:00

  • Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi.

    abstract::A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.7.2940-2943.1997

    authors: Goto M,Ekino K,Furukawa K

    更新日期:1997-07-01 00:00:00