High-rate anaerobic treatment of wastewater at low temperatures.

abstract：:A method is described for generating and screening variants of the beta-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus sensitive to several environmental stresses, with potential application in the food industry. Chemical mutagenesis with hydroxylamine or methoxylamine was performed on the beta-galacto...

journal_title：Applied and environmental microbiology

authors： Yoast S,Adams RM,Mainzer SE,Moon K,Palombella AL,Schmidt BF

更新日期：1994-04-01 00:00:00

abstract：:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (...

journal_title：Applied and environmental microbiology

authors： Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM

更新日期：1996-01-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:Two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. Annotation of the complete genome sequence of the probiotic bacterium Lactobacillus acidophilus NCFM revealed nine two-component regulatory systems. In this study, the histidine protein kinase of a two-compone...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,McAuliffe O,Altermann E,Lick S,Russell WM,Klaenhammer TR

更新日期：2005-10-01 00:00:00

abstract：:Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soil...

journal_title：Applied and environmental microbiology

authors： Velázquez E,Martínez-Romero E,Rodríguez-Navarro DN,Trujillo ME,Daza A,Mateos PF,Martínez-Molina E,van Berkum P

更新日期：2001-02-01 00:00:00

abstract：:Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...

journal_title：Applied and environmental microbiology

authors： González JM,Kiene RP,Moran MA

更新日期：1999-09-01 00:00:00

abstract：:To overcome the limited availability of antibiotic resistance markers in filamentous fungi, we adapted the FLP/FRT recombination system from the yeast Saccharomyces cerevisiae for marker recycling. We tested this system in the penicillin producer Penicillium chrysogenum using different experimental approaches. In a tw...

journal_title：Applied and environmental microbiology

authors： Kopke K,Hoff B,Kück U

更新日期：2010-07-01 00:00:00

abstract：:Legionella pneumophila is an intracellular parasite of protozoa and human phagocytes. To examine adaptation of this bacterium to parasitize protozoa, the sequence of events of the intracellular infection of the amoeba Hartmannella vermiformis was examined. The previously described uptake phenomenon of coiling phagocyt...

journal_title：Applied and environmental microbiology

authors： Abu Kwaik Y

更新日期：1996-06-01 00:00:00

abstract：:This study investigated the efficiency of methane and ammonium for stimulating trichloroethylene (TCE) biodegradation in groundwater microcosms (flasks and batch exchange columns) at a psychrophilic temperature (12 degrees C) typical of shallow aquifers in the northern United States or a mesophilic temperature (24 deg...

journal_title：Applied and environmental microbiology

authors： Moran BN,Hickey WJ

更新日期：1997-10-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40...

journal_title：Applied and environmental microbiology

authors： Cromeans TL,Kahler AM,Hill VR

更新日期：2010-02-01 00:00:00

abstract：:Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

journal_title：Applied and environmental microbiology

authors： Krishnavajhala A,Armstrong BA,Lopez JE

更新日期：2018-10-17 00:00:00

abstract：:Three cutinase gene-like genes from the basidiomycete Coprinopsis cinerea (Coprinus cinereus) found with a similarity search were cloned and expressed in Trichoderma reesei under the control of an inducible cbh1 promoter. The selected transformants of all three polyesterase constructs showed activity with p-nitropheny...

journal_title：Applied and environmental microbiology

authors： Kontkanen H,Westerholm-Parvinen A,Saloheimo M,Bailey M,Rättö M,Mattila I,Mohsina M,Kalkkinen N,Nakari-Setälä T,Buchert J

更新日期：2009-04-01 00:00:00

abstract：:The bacterial host ranges of the conjugatively self-transmissible IncN group plasmid pCU1 and its mobilizable miniderivative, pCU785, were examined. Species of the gram-negative purple bacteria were chosen for this study. Conjugative mobilization of pCU785 into a wide variety of bacteria was facilitated by the presenc...

journal_title：Applied and environmental microbiology

authors： Krishnan BR,Iyer VN

更新日期：1988-09-01 00:00:00

abstract：:Chlorpromazine was used to perform a test for the detection of microbial peroxidase activities. The compound acts as both a cell permeabilizer and a reagent in the procedure developed which allows the detection of peroxidase and peroxidase like reactions both semiquantitatively in whole cell determinations and quantit...

journal_title：Applied and environmental microbiology

authors： Galeazzi L,Turchetti G,Grilli G,Groppa G,Giunta S

更新日期：1986-12-01 00:00:00

abstract：:Although Pelobacter species are closely related to Geobacter species, recent studies suggested that Pelobacter carbinolicus may reduce Fe(III) via a different mechanism because it lacks the outer-surface c-type cytochromes that are required for Fe(III) reduction by Geobacter sulfurreducens. Investigation into the mech...

journal_title：Applied and environmental microbiology

authors： Haveman SA,DiDonato RJ Jr,Villanueva L,Shelobolina ES,Postier BL,Xu B,Liu A,Lovley DR

更新日期：2008-07-01 00:00:00

abstract：:Short-chain fatty acid (SCFA) biosynthesis is pertinent to production of biofuels, industrial compounds, and pharmaceuticals from renewable resources. To expand on Escherichia coli SCFA products, we previously implemented a coenzyme A (CoA)-dependent pathway that condenses acetyl-CoA to a diverse group of short-chain ...

journal_title：Applied and environmental microbiology

authors： McMahon MD,Prather KL

更新日期：2014-02-01 00:00:00

abstract：:Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. For this reason, another means of detecting this bacterium is necessary. In the pre...

journal_title：Applied and environmental microbiology

authors： Brauns LA,Hudson MC,Oliver JD

更新日期：1991-09-01 00:00:00

abstract：:Histological observations were made on the penetration of hyphae of Rhizopus oligosporus into soybean cotyledons in tempeh, an Indonesian soybean food. Hyphal penetrations averaged one per 1,400 mum (+/-390 mum) on the curved (outer) cotyledon surface and one per 1,010 mum (+/-340 mum) on the flat (inner) one. Hyphae ...

journal_title：Applied and environmental microbiology

authors： Jurus AM,Sundberg WJ

更新日期：1976-08-01 00:00:00

abstract：:Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the 69-amino-acid immunity peptide PepI. The N-terminal segment of PepI contains a 20-amino-acid stretch of apolar residues, whereas the...

journal_title：Applied and environmental microbiology

authors： Hoffmann A,Schneider T,Pag U,Sahl HG

更新日期：2004-06-01 00:00:00

abstract：:Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

journal_title：Applied and environmental microbiology

authors： Nielsen KM,Bones AM,Van Elsas JD

更新日期：1997-10-01 00:00:00

abstract：:Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

journal_title：Applied and environmental microbiology

authors： Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

更新日期：2009-01-01 00:00:00

abstract：:Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth....

journal_title：Applied and environmental microbiology

authors： Sadowsky MJ,Bohlool BB

更新日期：1986-10-01 00:00:00

abstract：:Desulfitobacterium hafniense strain TCE1 is capable of metabolically reducing tetra- and trichloroethenes by organohalide respiration. A previous study revealed that the pce gene cluster responsible for this process is located on an active composite transposon, Tn-Dha1. In the present work, we investigated the effects...

journal_title：Applied and environmental microbiology

authors： Duret A,Holliger C,Maillard J

更新日期：2012-09-01 00:00:00

abstract：:The routine production and storage of frozen doughs are still problematic. Although commercial baker's yeast is highly resistant to environmental stress conditions, it rapidly loses stress resistance during dough preparation due to the initiation of fermentation. As a result, the yeast loses gassing power significantl...

journal_title：Applied and environmental microbiology

authors： Teunissen A,Dumortier F,Gorwa MF,Bauer J,Tanghe A,Loïez A,Smet P,Van Dijck P,Thevelein JM

更新日期：2002-10-01 00:00:00

abstract：:The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA

更新日期：1989-12-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:Seventeen Bradyrhizobium sp. strains and one Azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16S rRNA gene (rDNA), (ii) randomly amplified DNA polymorphisms (RAPD) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv)...

journal_title：Applied and environmental microbiology

authors： Van Rossum D,Schuurmans FP,Gillis M,Muyotcha A,Van Verseveld HW,Stouthamer AH,Boogerd FC

更新日期：1995-04-01 00:00:00

abstract：:A mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. An organism, identified as Arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy s...

journal_title：Applied and environmental microbiology

authors： Marks TS,Smith AR,Quirk AV

更新日期：1984-11-01 00:00:00

abstract：:Aeromonas sp. will methylate trimethyl lead acetate (Me3PbOAc) to volatile tetramethyl lead (Me4Pb). Examination of cultures grown in the presence of Me3PbOAc revealed no major irregularities between cells of the treated and untreated cultures. Some cells, however, showed evidence that intracytoplasmic materials had b...

journal_title：Applied and environmental microbiology

authors： Silverberg BA,Wong PT,Chau YK

更新日期：1976-11-01 00:00:00