Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains.


:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (GAC)5 and (GTG)5 enabled discrimination between the strains tested. Additionally, restriction enzyme analysis, with TaqI and MseI, of PCR-amplified fragments from the complete internal transcribed spacer and nontranscribed spacer, both present in the rRNA-encoding gene cluster, proved to be suitable for generating intraspecies-specific patterns. Random amplified polymorphic DNA with primers 24 and OPA-11 and PCR fingerprinting with primer (GTG)5 appeared to generate the highest degree of diversity. However, the results indicated that there was no single PCR-mediated typing technique enabling discrimination on the strain level. Discrimination of each individual strain was nevertheless possible by combining the results obtained with all typing techniques.


Appl Environ Microbiol


Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM




Has Abstract


1996-01-01 00:00:00












  • Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England.

    abstract::The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

    更新日期:1998-04-01 00:00:00

  • Microbial communities and functional genes associated with soil arsenic contamination and the rhizosphere of the arsenic-hyperaccumulating plant Pteris vittata L.

    abstract::To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Xiong J,Wu L,Tu S,Van Nostrand JD,He Z,Zhou J,Wang G

    更新日期:2010-11-01 00:00:00

  • Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942.

    abstract::The impact of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene when used to transform the cyanobacterium Synechococcus sp. strain PCC 7942 has been examined. Maximal transcript and protein abundances were achieved by the addition of the lacZ promoter up...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Soltes-Rak E,Kushner DJ,Williams DD,Coleman JR

    更新日期:1993-08-01 00:00:00

  • Hemocytes are sites of enteric virus persistence within oysters.

    abstract::The goal of this study was to determine how enteric viruses persist within shellfish tissues. Several lines of novel evidence show that phagocytic blood cells (hemocytes) of Eastern oysters (Crassostrea virginica) play an important role in the retention of virus particles. Our results demonstrated an association of vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Provost K,Dancho BA,Ozbay G,Anderson RS,Richards GP,Kingsley DH

    更新日期:2011-12-01 00:00:00

  • PCR for bioaerosol monitoring: sensitivity and environmental interference.

    abstract::The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Alvarez AJ,Buttner MP,Stetzenbach LD

    更新日期:1995-10-01 00:00:00

  • Transformation of 2,4,6-Trinitrotoluene by Pseudomonas pseudoalcaligenes JS52.

    abstract::Pseudomonas pseudoalcaligenes JS52 grows on nitrobenzene via partial reduction of the nitro group and enzymatic rearrangement of the resultant hydroxylamine. Cells and cell extracts of nitrobenzene-grown JS52 catalyzed the transient formation of 4-hydroxylamino-2,6-dinitrotoluene (4HADNT), 4-amino-2,6-dinitrotoluene (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fiorella PD,Spain JC

    更新日期:1997-05-01 00:00:00

  • Seasonal cholera caused by Vibrio cholerae serogroups O1 and O139 in the coastal aquatic environment of Bangladesh.

    abstract::Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

    更新日期:2006-06-01 00:00:00

  • Prevalence and seasonality of tick-borne pathogens in questing Ixodes ricinus ticks from Luxembourg.

    abstract::In Europe, ixodid ticks are important arthropod vectors of human and animal pathogens, but comprehensive studies of the prevalence of all relevant pathogens in Central Europe are scarce. As a result of ecological changes, the incidences of tick-borne infections are expected to increase. In this study, 1,394 nymphal an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Reye AL,Hübschen JM,Sausy A,Muller CP

    更新日期:2010-05-01 00:00:00

  • Response of Sesbania grandiflora to Inoculation of Soil with Vesicular-Arbuscular Mycorrhizal Fungi.

    abstract::A greenhouse experiment was conducted to determine the influence of two tropical isolates of Glomus fasciculatum and Glomus mosseae on the nutrient uptake and growth of Sesbania grandiflora. Inoculation of sterile soil with the fungi significantly improved growth and nutrient uptake by S. grandiflora, but the response...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Habte M,Aziz T

    更新日期:1985-09-01 00:00:00

  • Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

    abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

    更新日期:2000-09-01 00:00:00

  • Utilization of ganglioside-degrading Paenibacillus sp. strain TS12 for production of glucosylceramide.

    abstract::Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sumida T,Sueyoshi N,Ito M

    更新日期:2002-11-01 00:00:00

  • Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by blaCTX-M on Washington State Dairy Farms.

    abstract::Enterobacteriaceae-associated blaCTX-M genes have become globally widespread within the past 30 years. Among isolates from Washington State cattle, Escherichia coli strains carrying blaCTX-M (CTX-M E. coli strains) were absent from a set of 2008 isolates but present in a set of isolates from 2011. On 30 Washington Sta...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Davis MA,Sischo WM,Jones LP,Moore DA,Ahmed S,Short DM,Besser TE

    更新日期:2015-07-01 00:00:00

  • Rapid aggregation of biofuel-producing algae by the bacterium Bacillus sp. strain RP1137.

    abstract::Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Powell RJ,Hill RT

    更新日期:2013-10-01 00:00:00

  • Development of a solid medium for growth and isolation of axenic microcystis strains (cyanobacteria).

    abstract::Solid media on a base of B-12 or CB medium with agarose or agarose of low melting temperature were developed for the cultivation of Microcystis species. The media with 0.4% gel showed the highest number of CFU, and increasing the gel concentration resulted in a reduction of the number of CFU. There was no difference i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Shirai M,Matumaru K,Ohotake A,Takamura Y,Aida T,Nakano M

    更新日期:1989-10-01 00:00:00

  • Characterization of an 8.7-kilobase thiostrepton resistance-encoding plasmid (pGIF3) of Streptomyces incarnatus.

    abstract::The low-copy-number 8.7-kb plasmid pGIF3 of Streptomyces incarnatus was studied after cloning in the Escherichia coli vector pBR322; a restriction map was constructed. Southern blot analysis showed that pGIF3 in S. incarnatus occurs predominantly as integrated in a larger replicon. The plasmid carries a gene for thios...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Malina H,Robert-Gero M

    更新日期:1992-03-01 00:00:00

  • Maintenance and induction of naphthalene degradation activity in Pseudomonas putida and an Alcaligenes sp. under different culture conditions.

    abstract::The expression of xenobiotic-degradative genes in indigenous bacteria or in bacteria introduced into an ecosystem is essential for the successful bioremediation of contaminated environments. The maintenance of naphthalene utilization activity is studied in Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. (strain...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Guerin WF,Boyd SA

    更新日期:1995-11-01 00:00:00

  • Activity of glutaraldehyde at low concentrations (less than 2%) against poliovirus and its relevance to gastrointestinal endoscope disinfection procedures.

    abstract::The activity of glutaraldehyde (GTA) at low concentrations (less than 2%) against poliovirus was assessed by a suspension procedure. The inactivation kinetics showed that concentrations of less than or equal to 0.10% were effective against purified poliovirus at pH 7.2; a 1 log10 reduction was obtained in 70 min with ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bailly JL,Chambon M,Peigue-Lafeuille H,Laveran H,De Champs C,Beytout D

    更新日期:1991-04-01 00:00:00

  • Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction.

    abstract::The growing mycelia of Trichoderma reesei Rut-C30 are richly endowed with endoplasmic reticula and a variety of pleomorphic subcellular bodies. Mycelia of the culture growing in presence of avicel pH101 was fractionated in sucrose density gradients, and several morphologically and biochemically distinct fractions were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Glenn M,Ghosh A,Ghosh BK

    更新日期:1985-11-01 00:00:00

  • Routes of intraspecies transmission of Mycobacterium avium subsp. paratuberculosis in rabbits (Oryctolagus cuniculus): a field study.

    abstract::Rabbits have been increasingly linked to the persistence of paratuberculosis (Johne's disease) in domestic ruminants in the United Kingdom. The aims of this study were to determine the routes of intraspecies transmission of Mycobacterium avium subspecies paratuberculosis (MAP) in rabbits and to estimate the probabilit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Judge J,Kyriazakis I,Greig A,Davidson RS,Hutchings MR

    更新日期:2006-01-01 00:00:00

  • Control of Biofilm Formation: Antibiotics and Beyond.

    abstract::Biofilm-associated bacteria are less sensitive to antibiotics than free-living (planktonic) cells. Furthermore, with variations in the concentration of antibiotics throughout a biofilm, microbial cells are often exposed to levels below inhibitory concentrations and may develop resistance. This, as well as the irrespon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审


    authors: Algburi A,Comito N,Kashtanov D,Dicks LMT,Chikindas ML

    更新日期:2017-01-17 00:00:00

  • β-Aminopeptidases: Insight into Enzymes without a Known Natural Substrate.

    abstract::β-Aminopeptidases have the unique capability to hydrolyze N-terminal β-amino acids, with varied preferences for the nature of β-amino acid side chains. This unique capability makes them useful as biocatalysts for synthesis of β-peptides and to kinetically resolve β-peptides and amides for the production of enantiopure...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: John-White M,Gardiner J,Johanesen P,Lyras D,Dumsday G

    更新日期:2019-07-18 00:00:00

  • AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.

    abstract::Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

    更新日期:2019-02-06 00:00:00

  • Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1.

    abstract::Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yieldin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hong HB,Chang YS,Nam IH,Fortnagel P,Schmidt S

    更新日期:2002-05-01 00:00:00

  • Destruction by anaerobic mesophilic and thermophilic digestion of viruses and indicator bacteria indigenous to domestic sludges.

    abstract::In raw sludges and in mesophilically and thermophilically digested anaerobic sludges, large variations in numbers of viruses occurred over narrow ranges of numbers of fecal coliforms, total coliforms, and fecal streptococci, demonstrating that the bacteria were poor quantitative reflectors of the numbers of the viruse...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Berg G,Berman D

    更新日期:1980-02-01 00:00:00

  • Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation.

    abstract::Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Zhang L,Sun C

    更新日期:2018-08-31 00:00:00

  • Enhancement of population size of a biological control agent and efficacy in control of bacterial speck of tomato through salicylate and ammonium sulfate amendments.

    abstract::Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ji P,Wilson M

    更新日期:2003-02-01 00:00:00

  • Environmental and taxonomic bacterial diversity of anaerobic uranium(IV) bio-oxidation.

    abstract::Microorganisms in diverse terrestrial surface and subsurface environments can anaerobically catalyze the oxidative dissolution of uraninite. While a limited quantity (∼5 to 12 μmol liter(-1)) of uranium is oxidatively dissolved in pure culture studies, the metabolism is coupled to electron transport, providing the pot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Weber KA,Thrash JC,Van Trump JI,Achenbach LA,Coates JD

    更新日期:2011-07-01 00:00:00

  • Effects of fixation on cell volume of marine planktonic protozoa.

    abstract::The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of p...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Choi JW,Stoecker DK

    更新日期:1989-07-01 00:00:00

  • Chromosomal location plays a role in regulation of aflatoxin gene expression in Aspergillus parasiticus.

    abstract::The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

    更新日期:2002-01-01 00:00:00

  • Use of controlled luciferase expression to monitor chemicals affecting protein synthesis.

    abstract::In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lampinen J,Virta M,Karp M

    更新日期:1995-08-01 00:00:00