Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains.

abstract：:Freshwater, sewage, and fecal samples from various sources were examined for Rhodococcus coprophilus, associated actinomycetes, Escherichia coli, and fecal streptococci. Rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater...

journal_title：Applied and environmental microbiology

authors： Mara DD,Oragui JI

更新日期：1981-12-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:Application of a mycorrhizal inoculum could be one way to increase the yield of rice plants and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading, and ripening stages in four paddy w...

journal_title：Applied and environmental microbiology

authors： Wang Y,Li T,Li Y,Björn LO,Rosendahl S,Olsson PA,Li S,Fu X

更新日期：2015-04-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

journal_title：Applied and environmental microbiology

authors： Tsuchiya K,Kimura T

更新日期：1978-04-01 00:00:00

abstract：:Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

journal_title：Applied and environmental microbiology

authors： Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

更新日期：2012-06-01 00:00:00

abstract：:Households provide environments that encourage the formation of microbial communities, often as biofilms. Such biofilms constitute potential reservoirs for pathogens, particularly for immune-compromised individuals. One household environment that potentially accumulates microbial biofilms is that provided by vinyl sho...

journal_title：Applied and environmental microbiology

authors： Kelley ST,Theisen U,Angenent LT,St Amand A,Pace NR

更新日期：2004-07-01 00:00:00

abstract：:Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host...

journal_title：Applied and environmental microbiology

authors： Ryu H,Griffith JF,Khan IU,Hill S,Edge TA,Toledo-Hernandez C,Gonzalez-Nieves J,Santo Domingo J

更新日期：2012-03-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:Strains of Bifidobacterium animalis subsp. lactis are extensively exploited by the food industry as health-promoting bacteria, although the genetic variability of members belonging to this taxon has so far not received much scientific attention. In this article, we describe the complete genetic makeup of the B. animal...

journal_title：Applied and environmental microbiology

authors： Milani C,Duranti S,Lugli GA,Bottacini F,Strati F,Arioli S,Foroni E,Turroni F,van Sinderen D,Ventura M

更新日期：2013-07-01 00:00:00

abstract：:The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...

journal_title：Applied and environmental microbiology

authors： Shiba T,Hill RT,Straube WL,Colwell RR

更新日期：1995-07-01 00:00:00

abstract：:Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ...

journal_title：Applied and environmental microbiology

authors： Asgeirsdóttir SA,Scholtmeijer K,Wessels JG

更新日期：1999-05-01 00:00:00

abstract：:Forty-seven strains representing 14 different Bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (IMF) and subsequently assessed for adherence to, invasion of, and cytotoxicity toward HEp-2 and Caco-2 cells. Cell-free supernatant fluids from 38 strains (81%) were ...

journal_title：Applied and environmental microbiology

authors： Rowan NJ,Deans K,Anderson JG,Gemmell CG,Hunter IS,Chaithong T

更新日期：2001-09-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:Starting with a strain of Bacillus cereus excreting about 40-fold more beta-amylase than does the original wild-type strain, we isolated, after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a strain designated BQ10-S1 SpoIII which showed under optimal conditions a further 5.5-fold increase in beta-amylase act...

journal_title：Applied and environmental microbiology

authors： Nanmori T,Numata Y,Shinke R

更新日期：1987-04-01 00:00:00

abstract：:The survival of bacteria exposed to toxic compounds is a multifactorial phenomenon, involving well-known molecular mechanisms of resistance but also less-well-understood mechanisms of tolerance that need to be clarified. In particular, the contribution of biofilm formation to survival in the presence of toxic compound...

journal_title：Applied and environmental microbiology

authors： Perrin C,Briandet R,Jubelin G,Lejeune P,Mandrand-Berthelot MA,Rodrigue A,Dorel C

更新日期：2009-03-01 00:00:00

abstract：:Fusarium oxysporum isolated from roots of and soil around Baccharis species from Brazil produced the trichothecenes T-2 toxin, HT-2 toxin, diacetoxyscirpenol, and 3'-OH T-2 (TC-1), whereas Fusarium sporotrichioides from the same source produced T-2 toxin, HT-2 toxin, acetyl T-2, neosolaniol, TC-1, 3'-OH HT-2 (TC-3), i...

journal_title：Applied and environmental microbiology

authors： Mirocha CJ,Abbas HK,Kommedahl T,Jarvis BB

更新日期：1989-01-01 00:00:00

abstract：:A gram-negative bacterium which hydrolyzed aryl N-methylcarbamate insecticides was isolated from an agricultural soil which quickly degraded these pesticides. This organism, designated strain ER2, grew on carbofuran as a sole source of carbon and nitrogen with a doubling time of 3 h in a mineral salts medium. The arom...

journal_title：Applied and environmental microbiology

authors： Topp E,Hanson RS,Ringelberg DB,White DC,Wheatcroft R

更新日期：1993-10-01 00:00:00

abstract：:The products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of Pseudomonas fluorescens Pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. Five analogs of rhizoxin, a 16-member macrolide w...

journal_title：Applied and environmental microbiology

authors： Loper JE,Henkels MD,Shaffer BT,Valeriote FA,Gross H

更新日期：2008-05-01 00:00:00

abstract：UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

journal_title：Applied and environmental microbiology

authors： Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

更新日期：2016-08-15 00:00:00

abstract：:Wastewater disinfection is used in many countries for reducing fecal coliform levels in effluents. Disinfection is therefore frequently used to improve recreational bathing waters which do not comply with microbiological standards. It is unknown whether human enteric viruses (which are responsible for waterborne disea...

journal_title：Applied and environmental microbiology

authors： Tree JA,Adams MR,Lees DN

更新日期：2003-04-01 00:00:00

abstract：:Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

journal_title：Applied and environmental microbiology

authors： Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

更新日期：1992-12-01 00:00:00

abstract：:Seabird ticks are known reservoirs of bacterial pathogens of medical importance; however, ticks parasitizing tropical seabirds have received less attention than their counterparts from temperate and subpolar regions. Recently, Rickettsia africae was described to infect seabird ticks of the western Indian Ocean and New...

journal_title：Applied and environmental microbiology

authors： Wilkinson DA,Dietrich M,Lebarbenchon C,Jaeger A,Le Rouzic C,Bastien M,Lagadec E,McCoy KD,Pascalis H,Le Corre M,Dellagi K,Tortosa P

更新日期：2014-06-01 00:00:00

abstract：:Physical agents, such as low electric voltage and current, have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric current was shown to suppress the growth of bacteria, it remains unclear to what extent the microampere current damaged the bacterial...

journal_title：Applied and environmental microbiology

authors： Krishnamurthi VR,Rogers A,Peifer J,Niyonshuti II,Chen J,Wang Y

更新日期：2020-08-03 00:00:00

abstract：:The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....

journal_title：Applied and environmental microbiology

authors： Murata H,Chatterjee A,Liu Y,Chatterjee AK

更新日期：1994-09-01 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

journal_title：Applied and environmental microbiology

authors： Alvarez AJ,Buttner MP,Stetzenbach LD

更新日期：1995-10-01 00:00:00

abstract：:Algal-bacterial mats which grow in the effluent channels of alkaline hot springs provided an environment suitable for studying natural thermophilic methane producing bacteria. Methane was rapidly produced in cores taken from the meat and appeared to be an end product of decomposition of the algal-bacterial organic mat...

journal_title：Applied and environmental microbiology

authors： Ward DM

更新日期：1978-06-01 00:00:00

abstract：:A method to detect viable Cryptosporidium parvum oocysts was developed. Polyclonal immunoglobulin G against C. parvum oocyst and sporozoite surface antigens was purified from rabbit immune serum, biotinylated, and bound to streptoavidin-coated magnetic particles. C. parvum oocysts were captured by a specific antigen-a...

journal_title：Applied and environmental microbiology

authors： Deng MQ,Cliver DO,Mariam TW

更新日期：1997-08-01 00:00:00