Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains.


:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (GAC)5 and (GTG)5 enabled discrimination between the strains tested. Additionally, restriction enzyme analysis, with TaqI and MseI, of PCR-amplified fragments from the complete internal transcribed spacer and nontranscribed spacer, both present in the rRNA-encoding gene cluster, proved to be suitable for generating intraspecies-specific patterns. Random amplified polymorphic DNA with primers 24 and OPA-11 and PCR fingerprinting with primer (GTG)5 appeared to generate the highest degree of diversity. However, the results indicated that there was no single PCR-mediated typing technique enabling discrimination on the strain level. Discrimination of each individual strain was nevertheless possible by combining the results obtained with all typing techniques.


Appl Environ Microbiol


Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM




Has Abstract


1996-01-01 00:00:00












  • Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp.

    abstract::The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Jain RK,Dreisbach JH,Spain JC

    更新日期:1994-08-01 00:00:00

  • Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.

    abstract::Cultures of rumen bacteria can be stored at -20 degrees C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Teather RM

    更新日期:1982-08-01 00:00:00

  • Metabolic engineering of Lactococcus lactis: influence of the overproduction of alpha-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions.

    abstract::The als gene for alpha-acetolactate synthase of Lactococcus lactis MG1363 was cloned on a multicopy plasmid under the control of the inducible L. lactis lacA promoter. More than a hundredfold overproduction of alpha-acetolactate synthase was obtained in L. lactis under inducing conditions as compared with that of the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Platteeuw C,Hugenholtz J,Starrenburg M,van Alen-Boerrigter I,de Vos WM

    更新日期:1995-11-01 00:00:00

  • Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.

    abstract::The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Llobet-Brossa E,Rosselló-Mora R,Amann R

    更新日期:1998-07-01 00:00:00

  • Carbon source preference in chemosynthetic hot spring communities.

    abstract::Rates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73 °C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Urschel MR,Kubo MD,Hoehler TM,Peters JW,Boyd ES

    更新日期:2015-06-01 00:00:00

  • Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.

    abstract::Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

    更新日期:1999-02-01 00:00:00

  • Lag phase of Salmonella enterica under osmotic stress conditions.

    abstract::Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Zhou K,George SM,Métris A,Li PL,Baranyi J

    更新日期:2011-03-01 00:00:00

  • Fate of Escherichia coli during ensiling of wheat and corn.

    abstract::A recombinant Escherichia coli strain carrying a plasmid with an antibiotic resistance marker and expressing the green fluorescent protein was inoculated at a concentration of 3.8 x 10(8) CFU/g into direct-cut wheat (348 g of dry matter kg(-1)), wilted wheat (450 g of dry matter kg(-1)), and corn (375 g of dry matter ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chen Y,Sela S,Gamburg M,Pinto R,Weinberg ZG

    更新日期:2005-09-01 00:00:00

  • Reengineering Escherichia coli for Succinate Production in Mineral Salts Medium.

    abstract::The fermentative metabolism of glucose was redirected to succinate as the primary product without mutating any genes encoding the native mixed-acid fermentation pathway or redox reactions. Two changes in peripheral pathways were together found to increase succinate yield fivefold: (i) increased expression of phosphoen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Zhang X,Jantama K,Shanmugam KT,Ingram LO

    更新日期:2009-12-01 00:00:00

  • Highly sensitive quantitative PCR for the detection and differentiation of Pseudogymnoascus destructans and other Pseudogymnoascus species.

    abstract::White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Shuey MM,Drees KP,Lindner DL,Keim P,Foster JT

    更新日期:2014-03-01 00:00:00

  • Effect of aeration and carbon dioxide on cell morphology of Candida utilis.

    abstract::The effect of CO2 availability on cell size, shape, and aggregation in continuous cultures of Candida utilis was studied in minimal medium with glucose or ethanol as the sole carbon and energy source. Enrichment with CO2 was achieved (i) by using the substrate with more C atoms, (ii) by using pure oxygen and thus decr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Straskrabová V,Páca J,Králícková E

    更新日期:1980-11-01 00:00:00

  • Deconjugation of bile acids by intestinal lactobacilli.

    abstract::Lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of Lactobacillus acidophilus deconjugated both glycocholate and taurocholate. All isolates of L. acidophilus from human feces deconjugated taurocholate, whereas only one of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gilliland SE,Speck ML

    更新日期:1977-01-01 00:00:00

  • Influence of the Gas-Water Interface on Transport of Microorganisms through Unsaturated Porous Media.

    abstract::In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wan J,Wilson JL,Kieft TL

    更新日期:1994-02-01 00:00:00

  • Community analysis of a mercury hot spring supports occurrence of domain-specific forms of mercuric reductase.

    abstract::Mercury is a redox-active heavy metal that reacts with active thiols and depletes cellular antioxidants. Active resistance to the mercuric ion is a widely distributed trait among bacteria and results from the action of mercuric reductase (MerA). Protein phylogenetic analysis of MerA in bacteria indicated the occurrenc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Simbahan J,Kurth E,Schelert J,Dillman A,Moriyama E,Jovanovich S,Blum P

    更新日期:2005-12-01 00:00:00

  • Hydrosol of Thymbra capitata Is a Highly Efficient Biocide against Salmonella enterica Serovar Typhimurium Biofilms.

    abstract:UNLABELLED:Salmonella is recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Karampoula F,Giaouris E,Deschamps J,Doulgeraki AI,Nychas GJ,Dubois-Brissonnet F

    更新日期:2016-08-15 00:00:00

  • Xylanase IV, an Exoxylanase of Aeromonas caviae ME-1 Which Produces Xylotetraose as the Only Low-Molecular-Weight Oligosaccharide from Xylan.

    abstract::A novel xylanase (xylanase IV) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of Aeromonas caviae ME-1. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase IV molecular weight was 41,000. Xylanase IV catalyze...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kubata BK,Takamizawa K,Kawai K,Suzuki T,Horitsu H

    更新日期:1995-04-01 00:00:00

  • Genomic and phylogenetic characterization of luminous bacteria symbiotic with the deep-sea fish Chlorophthalmus albatrossis (Aulopiformes: Chlorophthalmidae).

    abstract::Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate betwe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dunlap PV,Ast JC

    更新日期:2005-02-01 00:00:00

  • Development of an aerosol surface inoculation method for bacillus spores.

    abstract::A method was developed to deposit Bacillus subtilis spores via aerosolization onto various surface materials for biological agent decontamination and detection studies. This new method uses an apparatus coupled with a metered dose inhaler to reproducibly deposit spores onto various surfaces. A metered dose inhaler was...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lee SD,Ryan SP,Snyder EG

    更新日期:2011-03-01 00:00:00

  • Delignification of Wood Chips and Pulps by Using Natural and Synthetic Porphyrins: Models of Fungal Decay.

    abstract::Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Paszczyński A,Crawford RL,Blanchette RA

    更新日期:1988-01-01 00:00:00

  • Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria.

    abstract::Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chesson A,Stewart CS,Wallace RJ

    更新日期:1982-09-01 00:00:00

  • Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.

    abstract::Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identificat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Valentine N,Wunschel S,Wunschel D,Petersen C,Wahl K

    更新日期:2005-01-01 00:00:00

  • Conidiogenesis and secondary metabolism in Penicillium urticae.

    abstract::Submerged cultures of Penicillium urticae (NRRL 2159A) produced the antibiotics patulin and griseofulvin when grown in a glucose-nitrate medium. A high concentration of calcium (i.e., 68 mM) inhibited the production of both antibiotics while stimulating conidiogenesis. Conidial mutants that were defective in an early ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sekiguchi J,Gaucher GM

    更新日期:1977-01-01 00:00:00

  • Quantification of nitrogen reductase and nitrite reductase genes in soil of thinned and clear-cut Douglas-fir stands by using real-time PCR.

    abstract::The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Levy-Booth DJ,Winder RS

    更新日期:2010-11-01 00:00:00

  • AccR, a TetR Family Transcriptional Repressor, Coordinates Short-Chain Acyl Coenzyme A Homeostasis in Streptomyces avermitilis.

    abstract::Malonyl coenzyme A (malonyl-CoA) and methylmalonyl-CoA are the most common extender units for the biosynthesis of fatty acids and polyketides in Streptomyces, an industrially important producer of polyketides. Carboxylation of acetyl- and propionyl-CoAs is an essential source of malonyl- and methylmalonyl-CoAs; theref...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lyu M,Cheng Y,Han X,Wen Y,Song Y,Li J,Chen Z

    更新日期:2020-06-02 00:00:00

  • Split-Root Assays Using Trifolium subterraneum Show that Rhizobium Infection Induces a Systemic Response That Can Inhibit Nodulation of Another Invasive Rhizobium Strain.

    abstract::Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

    更新日期:1987-07-01 00:00:00

  • Poultry-associated Salmonella enterica subsp. enterica serovar 4,12:d:- reveals high clonality and a distinct pathogenicity gene repertoire.

    abstract::A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

    更新日期:2009-02-01 00:00:00

  • Kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification.

    abstract::The kinetics of denitrification and the causes of nitrite and nitrous oxide accumulation were examined in resting cell suspensions of three denitrifiers. An Alcaligenes species and a Pseudomonas fluorescens isolate characteristically accumulated nitrite when reducing nitrate; a Flavobacterium isolate did not. Nitrate ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Betlach MR,Tiedje JM

    更新日期:1981-12-01 00:00:00

  • Selective enrichment with a resuscitation step for isolation of freeze-injured Escherichia coli O157:H7 from foods.

    abstract::We studied injury of Escherichia coli O157:H7 cells in 11 food items during freeze storage and methods of isolating freeze-injured E. coli O157:H7 cells from foods. Food samples inoculated with E. coli O157:H7 were stored for 16 weeks at -20 degrees C in a freezer. Noninjured and injured cells were counted by using tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hara-Kudo Y,Ikedo M,Kodaka H,Nakagawa H,Goto K,Masuda T,Konuma H,Kojima T,Kumagai S

    更新日期:2000-07-01 00:00:00

  • Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.

    abstract::Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Desjardins AE,Plattner RD,Vanmiddlesworth F

    更新日期:1986-03-01 00:00:00

  • Characterization of wet-heat inactivation of single spores of bacillus species by dual-trap Raman spectroscopy and elastic light scattering.

    abstract::Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to investigate dynamic processes during high-temperature treatment of individual spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis in water. Major conclusions from these studies included the following. (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Zhang P,Kong L,Setlow P,Li YQ

    更新日期:2010-03-01 00:00:00