Application of the Saccharomyces cerevisiae FLP/FRT recombination system in filamentous fungi for marker recycling and construction of knockout strains devoid of heterologous genes.

abstract：:Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Gong F,Hedden P,Tudzynski B

更新日期：2008-09-01 00:00:00

abstract：:The effects of plasterboard composition on the growth and sporulation of Stachybotrys chartarum as well as on the inflammatory potential of the spores were studied. S. chartarum was grown on 13 modified plasterboards under saturated humidity conditions. The biomass was estimated by measuring the ergosterol content of ...

journal_title：Applied and environmental microbiology

authors： Murtoniemi T,Nevalainen A,Hirvonen MR

更新日期：2003-07-01 00:00:00

abstract：:Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhi...

journal_title：Applied and environmental microbiology

authors： Fan Z,Wagschal K,Chen W,Montross MD,Lee CC,Yuan L

更新日期：2009-03-01 00:00:00

abstract：:The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryp...

journal_title：Applied and environmental microbiology

authors： Marston CK,Hoffmaster AR,Wilson KE,Bragg SL,Plikaytis B,Brachman P,Johnson S,Kaufmann AF,Popovic T

更新日期：2005-12-01 00:00:00

abstract：:A high biomasses of Cladophora, a filamentous green alga, is found mainly during the summer along the shores of Lake Michigan. In this study, the abundance and persistence of the fecal indicator bacterium Escherichia coli and sulfate-reducing bacteria (SRB) on Cladophora mats collected at Lake Michigan beaches were ev...

journal_title：Applied and environmental microbiology

authors： Olapade OA,Depas MM,Jensen ET,McLellan SL

更新日期：2006-03-01 00:00:00

abstract：:Phragmidium violaceum causes leaf rust on the European blackberry (Rubus fruticosus L. aggregate). Multiple strains of this pathogen have been introduced into southern Australia for the biological control of at least 15 taxa of European blackberry, a nonindigenous, invasive plant. In climates conducive to leaf rust, t...

journal_title：Applied and environmental microbiology

authors： Gomez DR,Evans KJ,Baker J,Harvey PR,Scott ES

更新日期：2008-09-01 00:00:00

abstract：:Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...

journal_title：Applied and environmental microbiology

authors： Tempelaars MH,Rodrigues S,Abee T

更新日期：2011-04-01 00:00:00

abstract：:An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 20...

journal_title：Applied and environmental microbiology

authors： Anglès d'Auriac MB,Roberts H,Shaw T,Sirevåg R,Hermansen LF,Berg JD

更新日期：2000-10-01 00:00:00

abstract：:Thermostable and thermoactive beta-mannanase (1,4-beta-D-mannan mannanohydrolase [EC 3.2.1.78]), beta-mannosidase (beta-D-mannopyranoside hydrolase [EC 3.2.1.25]) and alpha-galactosidase (alpha-D-galactoside galactohydrolase [EC 3.2.1.22]) were purified to homogeneity from cell extracts and extracellular culture super...

journal_title：Applied and environmental microbiology

authors： Duffaud GD,McCutchen CM,Leduc P,Parker KN,Kelly RM

更新日期：1997-01-01 00:00:00

abstract：:Field-collected larvae of the tussock moth, Lymantria ninayi, a major pest of exotic pines in Papua New Guinea, were found to contain a small RNA-containing virus with a diameter of 29 nm and a buoyant density of 1.32 g/ml. The RNA was single stranded, had a molecular weight of 2.8 x 10, and was polyadenylated. Virion...

journal_title：Applied and environmental microbiology

authors： Pullin JS,Black F,King LA,Entwistle PF,Moore NF

更新日期：1984-09-01 00:00:00

abstract：:Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm sa...

journal_title：Applied and environmental microbiology

authors： Jindal A,Kocherginskaya S,Mehboob A,Robert M,Mackie RI,Raskin L,Zilles JL

更新日期：2006-12-01 00:00:00

abstract：:A novel serine carboxypeptidase (EC 3.4.16.1) was found in an Aspergillus oryzae fermentation broth and was purified to homogeneity. This enzyme has a molecular weight of ca. 67,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its specific activity is 21 U/mg for carbobenzoxy (Z)-Al...

journal_title：Applied and environmental microbiology

authors： Blinkovsky AM,Byun T,Brown KM,Golightly EJ

更新日期：1999-08-01 00:00:00

abstract：:The acetylene block technique was used to assay denitrification in undisturbed sediment cores of an intertidal mud flat. Nitrogen loss due to this process was estimated at 1 to 41 kg of N/hectare (ha) per year. Anaerobic nitrate-saturated slurry of the same sediment had a denitrification capacity of 1,026 kg of N/ha p...

journal_title：Applied and environmental microbiology

authors： Kaspar HF

更新日期：1982-03-01 00:00:00

abstract：:Enumeration of lactic acid streptococci from dairy starter cultures on Elliker agar was improved by the addition of 0.4% (wt/vol) diammonium phosphate. Agar plate counts were up to 7.75 times greater due to improved buffering by the phosphate salt. ...

journal_title：Applied and environmental microbiology

authors： Barach JT

更新日期：1979-07-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:Previous studies have demonstrated that reduced humic substances (HS) can be reoxidized by anaerobic bacteria such as Geobacter, Geothrix, and Wolinella species with a suitable electron acceptor; however, little is known of the importance of this metabolism in the environment. Recently we investigated this metabolism ...

journal_title：Applied and environmental microbiology

authors： Coates JD,Cole KA,Chakraborty R,O'Connor SM,Achenbach LA

更新日期：2002-05-01 00:00:00

abstract：:Mycobacterium vaccae can catabolize a number of major groundwater pollutants. When added singly, acetone, cyclohexane, styrene, benzene, ethylbenzene, propylbenzene, dioxane, and 1,2-dichloroethylene can be catabolized by M. vaccae. Catabolism of a number of these chemicals was monitored by gas-chromatographic analysi...

journal_title：Applied and environmental microbiology

authors： Burback BL,Perry JJ

更新日期：1993-04-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil...

journal_title：Applied and environmental microbiology

authors： Pontiroli A,Rizzi A,Simonet P,Daffonchio D,Vogel TM,Monier JM

更新日期：2009-05-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. ...

journal_title：Applied and environmental microbiology

authors： King AD Jr,Hocking AD,Pitt JI

更新日期：1979-05-01 00:00:00

abstract：UNLABELLED:In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mut...

journal_title：Applied and environmental microbiology

authors： Huertas-Rosales Ó,Ramos-González MI,Espinosa-Urgel M

更新日期：2016-08-30 00:00:00

abstract：:A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbon...

journal_title：Applied and environmental microbiology

authors： Hess A,Zarda B,Hahn D,Häner A,Stax D,Höhener P,Zeyer J

更新日期：1997-06-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:The physiology of ethanologenic Escherichia coli grown anaerobically in alkali-pretreated plant hydrolysates is complex and not well studied. To gain insight into how E. coli responds to such hydrolysates, we studied an E. coli K-12 ethanologen fermenting a hydrolysate prepared from corn stover pretreated by ammonia f...

journal_title：Applied and environmental microbiology

authors： Schwalbach MS,Keating DH,Tremaine M,Marner WD,Zhang Y,Bothfeld W,Higbee A,Grass JA,Cotten C,Reed JL,da Costa Sousa L,Jin M,Balan V,Ellinger J,Dale B,Kiley PJ,Landick R

更新日期：2012-05-01 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:Nitrification in chloraminated drinking water can have a number of adverse effects on water quality, including a loss of total chlorine and ammonia-N and an increase in the concentration of heterotrophic plate count bacteria and nitrite. To understand how nitrification develops, a study was conducted to examine the fa...

journal_title：Applied and environmental microbiology

authors： Wolfe RL,Lieu NI,Izaguirre G,Means EG

更新日期：1990-02-01 00:00:00