Adherence of Vibrio parahaemolyticus to human epithelial cell lines.

abstract：:Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

journal_title：Applied and environmental microbiology

authors： Kimura H,Sugihara M,Kato K,Hanada S

更新日期：2006-01-01 00:00:00

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...

journal_title：Applied and environmental microbiology

authors： Paszczyński A,Crawford RL,Blanchette RA

更新日期：1988-01-01 00:00:00

abstract：:The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

journal_title：Applied and environmental microbiology

authors： Enwall K,Philippot L,Hallin S

更新日期：2005-12-01 00:00:00

abstract：:Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as wa...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Villemur R

更新日期：2011-09-01 00:00:00

abstract：:An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This ra...

journal_title：Applied and environmental microbiology

authors： Mah RA,Smith MR,Baresi L

更新日期：1978-06-01 00:00:00

abstract：:Ruminal fluid from sheep fed hay plus concentrate diets containing 1.8% urea, 6% casein, or 6% egg albumin had proteolytic activities of 4.12, 3.02, or 4.00 mg of [14C]casein hydrolyzed ml-1 h-1, respectively. Dietary albumin had no effect on the rate of albumin breakdown relative to that of casein (0.06). Greater num...

journal_title：Applied and environmental microbiology

authors： Wallace RJ,Broderick GA,Brammall ML

更新日期：1987-04-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:The lysogenic bacteriophage APSE infects "Candidatus Hamiltonella defensa," a facultative endosymbiont of aphids and other sap-feeding insects. This endosymbiont has established a beneficial association with aphids, increasing survivorship following attack by parasitoid wasps. Although APSE and "Ca. Hamiltonella defen...

journal_title：Applied and environmental microbiology

authors： Degnan PH,Moran NA

更新日期：2008-11-01 00:00:00

abstract：:The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

journal_title：Applied and environmental microbiology

authors： Prince HN

更新日期：1978-08-01 00:00:00

abstract：:Adsorption of Agrobacterium tumefaciens cells to potato tuber disks reached equilibrium after coincubation for about 30 min. More than 10% of the number of bacteria bound at equilibrium were adsorbed within 30 s. Adsorption isotherms obtained at three temperatures showed that the heat of adsorption was nearly zero. ...

journal_title：Applied and environmental microbiology

authors： Kluepfel DA,Pueppke SG

更新日期：1986-05-01 00:00:00

abstract：:2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...

journal_title：Applied and environmental microbiology

authors： Dong W,Chen Q,Hou Y,Li S,Zhuang K,Huang F,Zhou J,Li Z,Wang J,Fu L,Zhang Z,Huang Y,Wang F,Cui Z

更新日期：2015-12-01 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or s...

journal_title：Applied and environmental microbiology

authors： Wegmüller B,Lüthy J,Candrian U

更新日期：1993-07-01 00:00:00

abstract：:The effects of plasterboard composition on the growth and sporulation of Stachybotrys chartarum as well as on the inflammatory potential of the spores were studied. S. chartarum was grown on 13 modified plasterboards under saturated humidity conditions. The biomass was estimated by measuring the ergosterol content of ...

journal_title：Applied and environmental microbiology

authors： Murtoniemi T,Nevalainen A,Hirvonen MR

更新日期：2003-07-01 00:00:00

abstract：:The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

journal_title：Applied and environmental microbiology

authors： Koo K,Foegeding PM,Swaisgood HE

更新日期：1998-07-01 00:00:00

abstract：:A long-chain aldehyde dehydrogenase, Ald1, was found in a soluble fraction of Acinetobacter sp. strain M-1 cells grown on n-hexadecane as a sole carbon source. The gene (ald1) was cloned from the chromosomal DNA of the bacterium. The open reading frame of ald1 was 1,512 bp long, corresponding to a protein of 503 amino...

journal_title：Applied and environmental microbiology

authors： Ishige T,Tani A,Sakai Y,Kato N

更新日期：2000-08-01 00:00:00

abstract：:Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

journal_title：Applied and environmental microbiology

authors： Khush RS,Becker E,Wach M

更新日期：1992-09-01 00:00:00

abstract：:The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2003-06-01 00:00:00

abstract：:The functioning of recirculation aquaculture systems (RAS) is essential to maintain water quality for fish health, and one crucial process here is nitrification. The investigated RAS was connected to a rainbow trout production system and operated at an average temperature of 13°C and pH 6.8. Community analyses of the ...

journal_title：Applied and environmental microbiology

authors： Hüpeden J,Wegen S,Off S,Lücker S,Bedarf Y,Daims H,Kühn C,Spieck E

更新日期：2016-01-08 00:00:00

abstract：:Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N2O production and methane oxidation in soils. Most of our knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. We have conducted a comparative study...

journal_title：Applied and environmental microbiology

authors： Jiang QQ,Bakken LR

更新日期：1999-06-01 00:00:00

abstract：:"Viili," a fermented milk product, has a firm but viscous consistency. It is produced with traditional mesophilic mixed-strain starters, which have various stabilities in dairy practice. Thirteen morphologically different types of phages were found in 90 viili samples studied by electron microscopy. Ten of the phage t...

journal_title：Applied and environmental microbiology

authors： Saxelin ML,Nurmiaho-Lassila EL,Meriläinen VT,Forsén RI

更新日期：1986-10-01 00:00:00

abstract：:Thiobacillus ferrooxidans AP19-3 oxidized molybdenum blue (Mo) enzymatically. Molybdenum oxidase in the plasma membrane of this bacterium was purified ca. 77-fold compared with molybdenum oxidase in cell extract. A purified molybdenum oxidase showed characteristic absorption maxima due to reduced-type cytochrome oxida...

journal_title：Applied and environmental microbiology

authors： Sugio T,Hirayama K,Inagaki K,Tanaka H,Tano T

更新日期：1992-05-01 00:00:00

abstract：:The extensive use of antibiotics in hospitals and in the animal breeding industry has promoted antibiotic resistance in bacteria, which resulted in the emergence of a large number of antibiotic resistance genes in the intestinal tract of human and farmed animals. Genetic exchange of resistance genes between the two ec...

journal_title：Applied and environmental microbiology

authors： Wang C,Song Y,Tang N,Zhang G,Leclercq SO,Feng J

更新日期：2020-12-11 00:00:00

abstract：:Some nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in sy...

journal_title：Applied and environmental microbiology

authors： Coby AJ,Picardal F,Shelobolina E,Xu H,Roden EE

更新日期：2011-09-01 00:00:00

abstract：:Although at least 13 antigenically distinct serotypes of Rhizobium leguminosarum bv. trifolii exist in an Abiqua silty clay loam soil, one serotype, AS6, occupies >/=50% of the root nodules formed on field-grown subclover and between 33 and 78% of the nodules formed on five annual clover species grown in the same soil...

journal_title：Applied and environmental microbiology

authors： Leung K,Yap K,Dashti N,Bottomley PJ

更新日期：1994-02-01 00:00:00

abstract：:Culture-independent (PCR with Crenarchaeota-specific primers and subsequent denaturing gradient gel electrophoresis) and culture-dependent approaches were used to study the diversity of Crenarchaeota in terrestrial hot springs of the Kamchatka Peninsula and the Lake Baikal region (Russia) and of Iceland. Among the phy...

journal_title：Applied and environmental microbiology

authors： Perevalova AA,Kolganova TV,Birkeland NK,Schleper C,Bonch-Osmolovskaya EA,Lebedinsky AV

更新日期：2008-12-01 00:00:00

abstract：:The solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. In common with other anaerobes, the clostridia show a marked dependence on the phosphoeno...

journal_title：Applied and environmental microbiology

authors： Al Makishah NH,Mitchell WJ

更新日期：2013-11-01 00:00:00

abstract：:Currently, it is estimated that only 0.001% to 15% of bacteria in any given system can be cultured by use of commonly used techniques and media, yet culturing is critically important for investigations of bacterial function. Despite this situation, few studies have attempted to link culture-dependent and culture-indep...

journal_title：Applied and environmental microbiology

authors： Walke JB,Becker MH,Hughey MC,Swartwout MC,Jensen RV,Belden LK

更新日期：2015-10-01 00:00:00

abstract：:The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

journal_title：Applied and environmental microbiology

authors： Stahl DA,Lane DJ,Olsen GJ,Pace NR

更新日期：1985-06-01 00:00:00