The shared resistome of human and pig microbiota is mobilized by distinct genetic elements.

abstract：:A rapid and simple approach to the small-subunit (SSU) rRNA-based quantitative detection of a specific group of microorganisms in complex ecosystems has been developed. The method employs sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H. Defined mixtures of SSU rRNAs were mixed with an ol...

journal_title：Applied and environmental microbiology

authors： Uyeno Y,Sekiguchi Y,Sunaga A,Yoshida H,Kamagata Y

更新日期：2004-06-01 00:00:00

abstract：:Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

journal_title：Applied and environmental microbiology

authors： Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

更新日期：2012-01-01 00:00:00

abstract：:A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

journal_title：Applied and environmental microbiology

authors： Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

更新日期：2014-12-01 00:00:00

abstract：:Seasonal variation in the phylogenetic composition of Synechococcus assemblages in estuarine and coastal waters of Hong Kong was examined through pyrosequencing of the rpoC1 gene. Sixteen samples were collected in 2009 from two stations representing estuarine and ocean-influenced coastal waters, respectively. Synechoc...

journal_title：Applied and environmental microbiology

authors： Xia X,Vidyarathna NK,Palenik B,Lee P,Liu H

更新日期：2015-11-01 00:00:00

abstract：:Proteins with high catalytic efficiency and selectivity under mild conditions have long been appreciated by industrial and medicinal fields. These proteins, which are commonly multimeric, often possess low stability, impeding wider application. Currently, strategies to improve the stability of multimeric proteins conc...

journal_title：Applied and environmental microbiology

authors： Zhu L,Wang S,Tian W,Zhang Y,Song Y,Zhang J,Mu B,Peng C,Deng Z,Ma H,Qu X

更新日期：2017-08-31 00:00:00

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater and seawater samples taken in Florida, the Gulf of Mexico, and the Bahamas Bank. Recoveries of T2 phage and calf thymus DNA added to artificial seawater and concentrated by VFF were 72.8 and 80%, respectively. Virus concentr...

journal_title：Applied and environmental microbiology

authors： Paul JH,Jiang SC,Rose JB

更新日期：1991-08-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:One of soil microbiology's most intriguing puzzles is how so many different bacterial species can coexist in small volumes of soil when competition theory predicts that less competitive species should decline and eventually disappear. We provide evidence supporting the theory that low pore connectivity caused by low w...

journal_title：Applied and environmental microbiology

authors： Carson JK,Gonzalez-Quiñones V,Murphy DV,Hinz C,Shaw JA,Gleeson DB

更新日期：2010-06-01 00:00:00

abstract：:The occurrence, structure, and mobility of Tn1546-like elements were studied in environmental vancomycin-resistant enterococci (VRE) isolated from municipal sewage, activated sludge, pharmaceutical waste derived from antibiotic production, seawater, blue mussels, and soil. Of 200 presumptive VRE isolates tested, 71 (3...

journal_title：Applied and environmental microbiology

authors： Guardabassi L,Dalsgaard A

更新日期：2004-02-01 00:00:00

abstract：:Nitrate, sulfate, and carbonate were used as electron acceptors to examine the anaerobic biodegradability of chlorinated aromatic compounds in estuarine and freshwater sediments. The respective denitrifying, sulfidogenic, and methanogenic enrichment cultures were established on each of the monochlorinated phenol and m...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Rivera MD,Young LY

更新日期：1993-04-01 00:00:00

abstract：:We investigated whether the yield of the B vitamin folic acid could be elevated in Bacillus subtilis. Strategies for increasing the folic acid yield were investigated by employing computer-aided flux analysis and mutation. Controlling the activity of the enzyme pyruvate kinase by placing it under inducible control was...

journal_title：Applied and environmental microbiology

authors： Zhu T,Pan Z,Domagalski N,Koepsel R,Ataai MM,Domach MM

更新日期：2005-11-01 00:00:00

abstract：:Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcrip...

journal_title：Applied and environmental microbiology

authors： Skraber S,Langlet J,Kremer JR,Mossong J,De Landtsheer S,Even J,Muller CP,Hoffmann L,Cauchie HM

更新日期：2011-08-01 00:00:00

abstract：:A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial spe...

journal_title：Applied and environmental microbiology

authors： Ventura M,Fitzgerald GF,van Sinderen D

更新日期：2005-10-01 00:00:00

abstract：:DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 ve...

journal_title：Applied and environmental microbiology

authors： Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya M

更新日期：2005-05-01 00:00:00

abstract：:A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

journal_title：Applied and environmental microbiology

authors： Chen H,Ponniah G,Salonen N,Blum P

更新日期：2004-08-01 00:00:00

abstract：:Iron is an essential micronutrient for life. In mammals, dietary iron is absorbed primarily in the small intestine. Currently, the impacts of dietary iron on the taxonomic structure and function of the gut microbiome and reciprocal effects on the animal host are not well understood. Here, we establish a mouse model of...

journal_title：Applied and environmental microbiology

authors： Coe GL,Pinkham NV,Celis AI,Johnson C,DuBois JL,Walk ST

更新日期：2021-01-15 00:00:00

abstract：:To elevate the production level of heterologous polyketide in Streptomyces venezuelae, an additional copy of the positive regulatory gene pikD was introduced into the pikromycin (Pik) polyketide synthase (PKS) deletion mutant of S. venezuelae ATCC 15439 expressing tylosin PKS genes. The resulting mutant strain showed ...

journal_title：Applied and environmental microbiology

authors： Jung WS,Jeong SJ,Park SR,Choi CY,Park BC,Park JW,Yoon YJ

更新日期：2008-04-01 00:00:00

abstract：:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbati...

journal_title：Applied and environmental microbiology

authors： Macario AJ,Conway de Macario E,Ney U,Schoberth SM,Sahm H

更新日期：1989-08-01 00:00:00

abstract：UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

journal_title：Applied and environmental microbiology

authors： Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

更新日期：2016-05-16 00:00:00

abstract：:A high biomasses of Cladophora, a filamentous green alga, is found mainly during the summer along the shores of Lake Michigan. In this study, the abundance and persistence of the fecal indicator bacterium Escherichia coli and sulfate-reducing bacteria (SRB) on Cladophora mats collected at Lake Michigan beaches were ev...

journal_title：Applied and environmental microbiology

authors： Olapade OA,Depas MM,Jensen ET,McLellan SL

更新日期：2006-03-01 00:00:00

abstract：:The virulence factor internalin A (InlA) facilitates the uptake of Listeria monocytogenes by epithelial cells that express the human isoform of E-cadherin. Previous studies identified naturally occurring premature stop codon (PMSC) mutations in inlA and demonstrated that these mutations are responsible for virulence a...

journal_title：Applied and environmental microbiology

authors： Van Stelten A,Nightingale KK

更新日期：2008-12-01 00:00:00

abstract：:Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fo...

journal_title：Applied and environmental microbiology

authors： Hemelt DM,Mares B,Upadhyay JM

更新日期：1979-09-01 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This ra...

journal_title：Applied and environmental microbiology

authors： Mah RA,Smith MR,Baresi L

更新日期：1978-06-01 00:00:00

abstract：:Three Methocel methylcellulose ethers of 1.9 degree of substitution with [14C]methyl labels were shown to be biodegradable using batch-type activated sludge tests. The maximum rate for conversion to 14CO2, attained after 1 week, was only 0.62 mg of methylcellulose/g of mixed liquor volatile solids per day. In 20 days,...

journal_title：Applied and environmental microbiology

authors： Blanchard FA,Takahashi IT,Alexander HC

更新日期：1976-10-01 00:00:00

abstract：:The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammoni...

journal_title：Applied and environmental microbiology

authors： Beaulieu M,Beaulieu Y,Melinard J,Pandian S,Goulet J

更新日期：1995-01-01 00:00:00

abstract：:Chickens on a broiler farm in southern England were found to be colonized with Campylobacter jejuni of a single serotype, Lior 1 Penner 4. The farm was the sole supplier of a local slaughterhouse associated with a campylobacter outbreak in 1984 caused by this serotype. The serotype persisted on the farm for at least 1...

journal_title：Applied and environmental microbiology

authors： Pearson AD,Greenwood M,Healing TD,Rollins D,Shahamat M,Donaldson J,Colwell RR

更新日期：1993-04-01 00:00:00

abstract：:Microbial partners play important roles in the biology and ecology of animals. In insects, maternally transmitted symbionts are especially common and can have host effects ranging from reproductive manipulation to nutrient provisioning and defense against natural enemies. In this study, we report a genus-wide associat...

journal_title：Applied and environmental microbiology

authors： Ballinger MJ,Moore LD,Perlman SJ

更新日期：2018-01-31 00:00:00