Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo.

Abstract:

:As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein of Malassezia furfur in the presence of shampoo. We found that phage display of llama single-domain antibody fragments (VHHs) can be extended to very harsh conditions, such as the presence of shampoo containing nonionic and anionic surfactants. We selected several VHHs that bind to the cell wall protein Malf1 of M. furfur, a fungus implicated in causing dandruff. In addition to high stability in the presence of shampoo, these VHHs are also stable under other denaturing conditions, such as high urea concentrations. Many of the stable VHHs were found to contain arginine at position 44. Replacement of the native amino acid at position 44 with arginine in the most stable VHH that lacked this arginine resulted in a dramatic further increase in the stability. The combination of the unique properties of VHHs together with applied phage display and protein engineering is a powerful method for obtaining highly stable VHHs that can be used in a wide range of applications.

journal_name

Appl Environ Microbiol

authors

Dolk E,van der Vaart M,Lutje Hulsik D,Vriend G,de Haard H,Spinelli S,Cambillau C,Frenken L,Verrips T

doi

10.1128/AEM.71.1.442-450.2005

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

442-50

issue

1

eissn

0099-2240

issn

1098-5336

pii

71/1/442

journal_volume

71

pub_type

杂志文章
  • Isolation and characterization of Arthrobacter bacteriophages and their application to phage typing of soil arthrobacters.

    abstract::Seventeen bacteriophages, active against 19 Arthrobacter soil isolates, were isolated from concentrated samples of river water and sewage. Attempts to isolate Arthrobacter bacteriophages from filtrates of broth cultures of the soil isolates or from ultraviolet light-irradiated cultures were unsuccessful. Bacteriophage...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.1.185-191.1978

    authors: Brown DR,Holt JG,Pattee PA

    更新日期:1978-01-01 00:00:00

  • Intracellular proliferation of Legionella pneumophila in Hartmannella vermiformis in aquatic biofilms grown on plasticized polyvinyl chloride.

    abstract::The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo growth of L. pneumophila in protozoa in aquatic biofilms developing at high concentrations on plasticized polyvinyl chloride...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6826-6833.2004

    authors: Kuiper MW,Wullings BA,Akkermans AD,Beumer RR,van der Kooij D

    更新日期:2004-11-01 00:00:00

  • Ribosome-Engineered Lacticaseibacillus rhamnosus Strain GG Exhibits Cell Surface Glyceraldehyde-3-Phosphate Dehydrogenase Accumulation and Enhanced Adhesion to Human Colonic Mucin.

    abstract::Differences in individual host responses have emerged as an issue regarding the health benefits of probiotics. Here, we applied ribosome engineering (RE) technology, developed in an actinomycete study, to Lacticaseibacillus rhamnosus GG (LGG). RE can effectively enhance microbial potential by using antibiotics to indu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01448-20

    authors: Ishida M,Namai F,Shigemori S,Kajikawa S,Tsukagoshi M,Sato T,Ogita T,Shimosato T

    更新日期:2020-10-01 00:00:00

  • Insights into the Function of the N-Acetyltransferase SatA That Detoxifies Streptothricin in Bacillus subtilis and Bacillus anthracis.

    abstract::Acylation of epsilon amino groups of lysyl side chains is a widespread modification of proteins and small molecules in cells of all three domains of life. Recently, we showed that Bacillus subtilis and Bacillus anthracis encode the GCN5-related N-acetyltransferase (GNAT) SatA that can acetylate and inactivate streptot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03029-18

    authors: Burckhardt RM,Escalante-Semerena JC

    更新日期:2019-03-06 00:00:00

  • Novel Sampling Method for Assessing Human-Pathogen Interactions in the Natural Environment Using Boot Socks and Citizen Scientists, with Application to Campylobacter Seasonality.

    abstract::This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00162-17

    authors: Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

    更新日期:2017-06-30 00:00:00

  • Cell Surface Characteristics of Bacteriophage-Resistant Lactococcus lactis subsp. cremoris SK110 and Its Bacteriophage-Sensitive Variant SK112.

    abstract::Several cell surface characteristics of bacteriophage-resistant Lactococcus lactis subsp. cremoris SK110 were compared with those of its phage-sensitive derivative SK112. After centrifugation, SK110 cells resisted suspension more strongly than SK112 cells. SK112 was more negatively charged and had a more hydrophobic c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.10.3230-3233.1990

    authors: Sijtsma L,Jansen N,Hazeleger WC,Wouters JT,Hellingwerf KJ

    更新日期:1990-10-01 00:00:00

  • Genetic diversity and horizontal transfer of genes involved in oxidation of reduced phosphorus compounds by Alcaligenes faecalis WM2072.

    abstract::Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of Alcaligenes faecalis. The genes required for oxidation of hypophosphi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.1.290-296.2005

    authors: Wilson MM,Metcalf WW

    更新日期:2005-01-01 00:00:00

  • Microbial transformation of 14C-labeled 2,4,6-trinitrotoluene in an activated-sludge system.

    abstract::The fate of 14C-labeled 2,4,6-trinitrotoluene (TNT) in an activated-sludge system was investigated. No [14C]TNT could be detected in the contents of an aerated reactor after 3 to 5 days of incubation. No significant 14CO2 was formed, and the radioactivity was about equally divided between the floc and the supernatant....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.5.949-954.1978

    authors: Carpenter DF,McCormick NG,Cornell JH,Kaplan AM

    更新日期:1978-05-01 00:00:00

  • Evidence for iron-dependent nitrate respiration in the dissimilatory iron-reducing bacterium Geobacter metallireducens.

    abstract::The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximatel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3750-3752.2001

    authors: Senko JM,Stolz JF

    更新日期:2001-08-01 00:00:00

  • Comparison of antibody production against aflatoxin B1 in goats and rabbits.

    abstract::Antibody production against aflatoxin B1 was compared in three rabbits and one goat. Titers obtained were 20 times higher in the rabbits than in the goat. The goat antiserum appeared to have a higher degree of cross-reactivity for other aflatoxins and related metabolites than did the rabbit antiserum. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.3.678-680.1980

    authors: Gaur PK,El-Nakib O,Chu FS

    更新日期:1980-09-01 00:00:00

  • Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens.

    abstract::Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00757-16

    authors: Quelas JI,Mesa S,Mongiardini EJ,Jendrossek D,Lodeiro AR

    更新日期:2016-06-30 00:00:00

  • Stereochemistry of furfural reduction by a Saccharomyces cerevisiae aldehyde reductase that contributes to in situ furfural detoxification.

    abstract::Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00542-10

    authors: Bowman MJ,Jordan DB,Vermillion KE,Braker JD,Moon J,Liu ZL

    更新日期:2010-08-01 00:00:00

  • Fundamental procedures for determining ergosterol content of decaying plant material by liquid chromatography.

    abstract::Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1876-1879.1988

    authors: Newell SY,Arsuffi TL,Fallon RD

    更新日期:1988-07-01 00:00:00

  • Tremorgenic mycotoxin from Penicillium paraherquei.

    abstract::A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.3.441-442.1976

    authors: Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

    更新日期:1976-09-01 00:00:00

  • Evaluation of nongermicidal handwashing protocols for removal of transient microbial flora.

    abstract::A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.5.1067-1071.1985

    authors: Vesley D,Lillquist DR,Le CT

    更新日期:1985-05-01 00:00:00

  • Hypoxic adaptation by Efg1 regulates biofilm formation by Candida albicans.

    abstract::Hypoxia is encountered frequently by Candida albicans during systemic infection of the human host. We tested if hypoxia allows biofilm formation by C. albicans, which is a major cause of perseverance and antifungal resistance in C. albicans infections. Using an in vitro biofilm system, we unexpectedly discovered that ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00098-09

    authors: Stichternoth C,Ernst JF

    更新日期:2009-06-01 00:00:00

  • Virus movement in soil columns flooded with secondary sewage effluent.

    abstract::Secondary sewage effluent containing about 3 X 10(4) plaque-forming units of polio virus type 1 (LSc) per ml was passed through columns 250 cm in length packed with calcareous sand from an area in the Salt River bed used for ground-water recharge of secondary sewage effluent. Viruses were not detected in 1-ml samples ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.4.520-526.1976

    authors: Lance JC,Gerba CP,Melnick JL

    更新日期:1976-10-01 00:00:00

  • Biphasic thermal inactivation kinetics in Salmonella enteritidis PT4.

    abstract::The thermal inactivation kinetics of Salmonella enteritidis PT4 between 49 and 60 degrees C were investigated. Using procedures designed to eliminate methodological artifacts, we found that the death kinetics deviated from the accepted model of first-order inactivation. When we used high-density stationary-phase popul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.459-464.1998

    authors: Humpheson L,Adams MR,Anderson WA,Cole MB

    更新日期:1998-02-01 00:00:00

  • Evolutionary relationships of "Candidatus endobugula" bacterial symbionts and their Bugula bryozoan hosts.

    abstract::Ribosomal gene sequences were obtained from bryozoans in the genus Bugula and their bacterial symbionts; analyses of host and symbiont phylogenetic trees did not support a history of strict cospeciation. Symbiont-derived compounds known to defend host larvae from predation were only detected in two out of four symbiot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02798-07

    authors: Lim-Fong GE,Regali LA,Haygood MG

    更新日期:2008-06-01 00:00:00

  • Chromium reduction in Pseudomonas putida.

    abstract::Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat lab...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.7.2268-2270.1990

    authors: Ishibashi Y,Cervantes C,Silver S

    更新日期:1990-07-01 00:00:00

  • A proposed pathway for catabolism of propionate in methanogenic cocultures.

    abstract::A metabolic pathway for the catabolism of propionate is proposed. This pathway incorporates a transcarboxylation reaction involving propionyl coenzyme A and oxaloacetate and a carboxylation of pyruvate to regenerate oxaloacetate. Data indicated that the proposed pathway is reversible. The proposed pathway and its appa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1300-1301.1988

    authors: Robbins JE

    更新日期:1988-05-01 00:00:00

  • Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli.

    abstract::Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.8.2758-2759.1993

    authors: Haines JR,Covert TC,Rankin CC

    更新日期:1993-08-01 00:00:00

  • Identification of Enterococcus spp. with a biochemical key.

    abstract::A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.10.4425-4430.1999

    authors: Manero A,Blanch AR

    更新日期:1999-10-01 00:00:00

  • Differential role of passerine birds in distribution of Borrelia spirochetes, based on data from ticks collected from birds during the postbreeding migration period in Central Europe.

    abstract::Borrelia spirochetes in bird-feeding ticks were studied in the Czech Republic. During the postbreeding period (July to September 2005), 1,080 passerine birds infested by 2,240 Ixodes ricinus subadult ticks were examined. Borrelia garinii was detected in 22.2% of the ticks, Borrelia valaisiana was detected in 12.8% of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01674-08

    authors: Dubska L,Literak I,Kocianova E,Taragelova V,Sychra O

    更新日期:2009-02-01 00:00:00

  • Transcription of hupSL in Anabaena variabilis ATCC 29413 is regulated by NtcA and not by hydrogen.

    abstract::Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02855-07

    authors: Weyman PD,Pratte B,Thiel T

    更新日期:2008-04-01 00:00:00

  • Inactivation of indigenous viruses in raw sludge by air drying.

    abstract::Air drying of raw sludge caused inactivation of indigenous viruses. A gradual loss of infectivity occurred with the loss of water until the solids content reached about 80%. A more rapid decline of viral infectivity occurred with further dewatering. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.6.1943-1945.1983

    authors: Brashear DA,Ward RL

    更新日期:1983-06-01 00:00:00

  • Saccharomyces cerevisiae genome shuffling through recursive population mating leads to improved tolerance to spent sulfite liquor.

    abstract::Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02769-10

    authors: Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

    更新日期:2011-07-01 00:00:00

  • Genetic rearrangement strategy for optimizing the dibenzothiophene biodesulfurization pathway in Rhodococcus erythropolis.

    abstract::Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02319-07

    authors: Li GQ,Li SS,Zhang ML,Wang J,Zhu L,Liang FL,Liu RL,Ma T

    更新日期:2008-02-01 00:00:00

  • Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway.

    abstract::Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.740-746.2003

    authors: Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

    更新日期:2003-02-01 00:00:00

  • Purification and characterization of a pyruvated-mannose-specific xanthan lyase from heat-stable, salt-tolerant bacteria.

    abstract::A xanthanase complex secreted by a consortium of heat-stable, salt-tolerant bacteria includes a lyase that specifically removes terminal pyruvated beta-d-mannose residues from the side chains of xanthan gum. The enzyme was purified to homogeneity from the culture broth following ion-exchange chromatography and gel per...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2523-2528.1991

    authors: Ahlgren JA

    更新日期:1991-09-01 00:00:00