Increase in acid tolerance of Campylobacter jejuni through coincubation with amoebae.

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:Communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in freshwater sediments and those in association with the root system of the macrophyte species Littorella uniflora, Juncus bulbosus, and Myriophyllum alterniflorum were compared for seven oligotrophic to mesotrophic softwater lakes and acidic heathland...

journal_title：Applied and environmental microbiology

authors： Herrmann M,Saunders AM,Schramm A

更新日期：2009-05-01 00:00:00

abstract：:The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

journal_title：Applied and environmental microbiology

authors： Li SY,Chang SC,Wei RD

更新日期：1985-09-01 00:00:00

abstract：:The lantibiotic (lanthionine-containing antibiotic) mersacidin is an antimicrobial peptide consisting of 20 amino acids and is produced by Bacillus sp. strain HIL Y-85,54728. The structural gene (mrsA) and the genes for producer self-protection, modification enzymes, transport proteins, and regulator proteins are orga...

journal_title：Applied and environmental microbiology

authors： Schmitz S,Hoffmann A,Szekat C,Rudd B,Bierbaum G

更新日期：2006-11-01 00:00:00

abstract：:Bacterial utilization of crude oil components, such as the n-alkanes, requires complex cell surface adaptation to allow adherence to oil. To better understand microbial cell surface adaptation to growth on crude oil, the cell surface characteristics of two Pseudomonas aeruginosa strains, U1 and U3, both isolated from ...

journal_title：Applied and environmental microbiology

authors： Norman RS,Frontera-Suau R,Morris PJ

更新日期：2002-10-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:The organization and species composition of bacterial trophic groups associated with lactose biomethanation were investigated in a whey-processing chemostat by enumeration, isolation, and general characterization studies. The bacteria were spatially organized as free-living forms and as self-immobilized forms appearin...

journal_title：Applied and environmental microbiology

authors： Chartrain M,Zeikus JG

更新日期：1986-01-01 00:00:00

abstract：:DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...

journal_title：Applied and environmental microbiology

authors： Louws FJ,Fulbright DW,Stephens CT,de Bruijn FJ

更新日期：1994-07-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:Although the high level of competence for natural transformation of Acinetobacter sp. strain BD413 has been the subject of numerous studies, only two competence genes, comC and comP, have been identified to date. By chromosomal walking analysis we found two overlapping open reading frames, designated comE and comF, st...

journal_title：Applied and environmental microbiology

authors： Busch S,Rosenplänter C,Averhoff B

更新日期：1999-10-01 00:00:00

abstract：:A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H(2)S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H(2)S in a synthetic juice mediu...

journal_title：Applied and environmental microbiology

authors： Linderholm AL,Findleton CL,Kumar G,Hong Y,Bisson LF

更新日期：2008-03-01 00:00:00

abstract：:The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicro...

journal_title：Applied and environmental microbiology

authors： Scupham AJ,Jones JA,Rettedal EA,Weber TE

更新日期：2010-12-01 00:00:00

abstract：:Efficient enrichment of staphylococcal cells displaying specific heterologous affinity ligands on their cell surfaces was demonstrated by using fluorescence-activated cell sorting. Using bacterial surface display of peptide or protein libraries for the purpose of combinatorial protein engineering has previously been i...

journal_title：Applied and environmental microbiology

authors： Wernérus H,Samuelson P,Ståhl S

更新日期：2003-09-01 00:00:00

abstract：:The undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of Listeria spp. In some cases, these microbial consortia inhibit Listeria almost completely. From such undefined industrial cheese-ripening floras, ...

journal_title：Applied and environmental microbiology

authors： Eppert I,Valdés-Stauber N,Götz H,Busse M,Scherer S

更新日期：1997-12-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:The Lactobacillus johnsonii VPI 11088 groESL operon was localized on the chromosome near the insertion element IS1223. The operon was initially cloned as a series of three overlapping PCR fragments, which were sequenced and used to design primers to amplify the entire operon. The amplified fragment was used as a probe...

journal_title：Applied and environmental microbiology

authors： Walker DC,Girgis HS,Klaenhammer TR

更新日期：1999-07-01 00:00:00

abstract：:Pseudomonas syringae is a common foliar bacterium responsible for many important plant diseases. We studied the population structure and dynamics of the core genome of P. syringae via multilocus sequencing typing (MLST) of 60 strains, representing 21 pathovars and 2 nonpathogens, isolated from a variety of plant hosts...

journal_title：Applied and environmental microbiology

authors： Sarkar SF,Guttman DS

更新日期：2004-04-01 00:00:00

abstract：:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance. Therefore, benzene metabolism was investigated in Geobacter metallireducens, the only genetically tractable organism known to anaerobically degrade benzene. Trace amounts (<0.5 μM) of phenol accumulated in cult...

journal_title：Applied and environmental microbiology

authors： Zhang T,Tremblay PL,Chaurasia AK,Smith JA,Bain TS,Lovley DR

更新日期：2013-12-01 00:00:00

abstract：:A novel agar medium, chromogenic Salmonella esterase (CSE) agar, for the differentiation of salmonellae is described. The agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3, 5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl ca...

journal_title：Applied and environmental microbiology

authors： Cooke VM,Miles RJ,Price RG,Richardson AC

更新日期：1999-02-01 00:00:00

abstract：:In two intertidal marshes, the vertical distribution in the sediment and inhibition by ammonium of methane oxidation were investigated by slurry incubation experiments. The two sites differ in their dominant vegetation type, i.e., reed and bulrush, and in their heights above sea level. The reed site was elevated with ...

journal_title：Applied and environmental microbiology

authors： Van Der Nat F,De Brouwer J,Middelburg JJ,Laanbroek HJ

更新日期：1997-12-01 00:00:00

abstract：:Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...

journal_title：Applied and environmental microbiology

authors： Belser LW,Schmidt EL

更新日期：1978-10-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, e...

journal_title：Applied and environmental microbiology

authors： Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

更新日期：2011-07-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol...

journal_title：Applied and environmental microbiology

authors： Dikicioglu D,Pir P,Onsan ZI,Ulgen KO,Kirdar B,Oliver SG

更新日期：2008-09-01 00:00:00

abstract：:Sigma B (sigma(B)) is a stress-responsive alternative sigma factor that has been identified in various gram-positive bacteria. Seven different regulators of sigma B (Rsbs) are located in the sigB operons of both Bacillus subtilis and Listeria monocytogenes. In B. subtilis, these proteins contribute to regulation of si...

journal_title：Applied and environmental microbiology

authors： Chaturongakul S,Boor KJ

更新日期：2004-09-01 00:00:00

abstract：:This study was initiated to determine if reduced sulfur contained in coal gasifier ash and slag was available for microbial and chemical oxidation because eventual large-quantity landfill disposal of these solid wastes is expected. Continuous application of distilled water to a column containing a high-sulfur-content ...

journal_title：Applied and environmental microbiology

authors： Strayer RF,Davis EC

更新日期：1983-03-01 00:00:00

abstract：:Essential oil components (EOCs) have limited water solubility and are used at much higher concentrations in complex food matrices than in growth media to inhibit pathogens. However, the correlation between solubility and activity has not been studied. The objective of this work was to characterize the solubility of EO...

journal_title：Applied and environmental microbiology

authors： Chen H,Davidson PM,Zhong Q

更新日期：2014-02-01 00:00:00

abstract：:A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

journal_title：Applied and environmental microbiology

authors： Chen H,Ponniah G,Salonen N,Blum P

更新日期：2004-08-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00