Fluorescence-activated cell sorting of specific affibody-displaying staphylococci.

abstract：:Ralstonia solanacearum, a major phytopathogenic bacterium, causes a bacterial wilt disease in diverse plants. Although fatty acid analyses of total membranes of R. solanacearum showed that they contain primarily palmitic (C(16:0)), palmitoleic (C(16:1)) and cis-vaccenic (C(18:1)) acids, little is known regarding R. so...

journal_title：Applied and environmental microbiology

authors： Cheng J,Ma J,Lin J,Fan ZC,Cronan JE,Wang H

更新日期：2012-03-01 00:00:00

abstract：:Growth of the ruminal bacteria Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and R. albus 7 followed Monod kinetics with respect to concentrations of individual pure cellodextrins (cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose). Under the conditions tested, R. flavefaciens FD-...

journal_title：Applied and environmental microbiology

authors： Shi Y,Weimer PJ

更新日期：1996-03-01 00:00:00

abstract：:The multistep cleavage of carotenoids in Mucorales during the sexual phase results in a cocktail of trisporic acid (C18) sex pheromones. We hypothesized that the C18 trisporoid intermediates have a specific regulatory function for sex pheromone production and carotenogenesis that varies with genus/species and vegetati...

journal_title：Applied and environmental microbiology

authors： Sahadevan Y,Richter-Fecken M,Kaerger K,Voigt K,Boland W

更新日期：2013-12-01 00:00:00

abstract：:The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

journal_title：Applied and environmental microbiology

authors： Ford LM,Eaton TE,Godfrey OW

更新日期：1990-11-01 00:00:00

abstract：:Electrophoretic karyotypes (EKs) of 83 isolates were variable within agricultural and natural populations of Sclerotinia sclerotiorum, as well as among S. sclerotiorum, Sclerotinia minor, and Sclerotinia trifoliorum. Variation in EKs was not observed within six mitotic or three meiotic lineages of isolates. EKs of 8 t...

journal_title：Applied and environmental microbiology

authors： Errampalli D,Kohn LM

更新日期：1996-11-01 00:00:00

abstract：:Lactic and acetic acids were determined to be slightly synergistic in their inhibitory interrelationship against Salmonella typhimurium with the use of a modified toxicological model. ...

journal_title：Applied and environmental microbiology

authors： Rubin HE

更新日期：1978-10-01 00:00:00

abstract：:This study demonstrates that elution of enteroviruses from a mixture of primary- and activated-sludge solids with beef extract at pH 9.2 +/- 0.2 may be less efficient than elution with beef extract at pH 7.2 +/- 0.2 and that elution of enteroviruses from extended-aeration-sludge solids with beef extract is at best no ...

journal_title：Applied and environmental microbiology

authors： Berg G,Sullivan G

更新日期：1988-07-01 00:00:00

abstract：:Arthrobacter oxydans HAP-1 hyperproduces DL-alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by L-alanine dehydrogenase. We pr...

journal_title：Applied and environmental microbiology

authors： Hashimoto Si,Katsumata R

更新日期：1999-06-01 00:00:00

abstract：:Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia. The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product. Since the presence of the product did not ind...

journal_title：Applied and environmental microbiology

authors： Abbaszadegan M,Huber MS,Gerba CP,Pepper IL

更新日期：1997-01-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:The biodegradation of benzene, toluene, and chlorobenzenes by Pseudomonas putida involves the initial conversion of the parent molecules to cis-dihydrodiols by dioxygenase enzyme systems. The cis-dihydrodiols are then converted to the corresponding catechols by dihydrodiol dehydrogenase enzymes. Pseudomonas sp. strain...

journal_title：Applied and environmental microbiology

authors： Spain JC,Gibson DT

更新日期：1988-06-01 00:00:00

abstract：:Vibrio cholerae can shift to a "rugose" phenotype, thereby producing copious exopolysaccharide (EPS), which promotes its environmental survival and persistence. We report conditions that promote high-frequency rugose EPS production (HFRP), whereby cells switch at high frequency (up to 80%) to rugose EPS production. HF...

journal_title：Applied and environmental microbiology

authors： Ali A,Rashid MH,Karaolis DK

更新日期：2002-11-01 00:00:00

abstract：:Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium f...

journal_title：Applied and environmental microbiology

authors： Monteiro PB,Teixeira DC,Palma RR,Garnier M,Bové JM,Renaudin J

更新日期：2001-05-01 00:00:00

abstract：:The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...

journal_title：Applied and environmental microbiology

authors： Phelps PA,Agarwal SK,Speitel GE,Georgiou G

更新日期：1992-11-01 00:00:00

abstract：:The probability of recovering pathogenic Escherichia coli from food by the Bacteriological Analytical Manual method was determined by the effects of several factors: the number of strains per food, the ability of pathogenic strains to survive enrichment, and the frequency of plasmid loss during enrichment. Biochemical...

journal_title：Applied and environmental microbiology

authors： Hill WE,Ferreira JL,Payne WL,Jones VM

更新日期：1985-06-01 00:00:00

abstract：:Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

journal_title：Applied and environmental microbiology

authors： Murphy SK,Oliver JD

更新日期：1992-09-01 00:00:00

abstract：:The host range and transfer frequency of an IncP-1 plasmid (pKJK10) among indigenous bacteria in the barley rhizosphere was investigated. A new flow cytometry-based cultivation-independent method for enumeration and sorting of transconjugants for subsequent 16S rRNA gene classification was used. Indigenous transconjug...

journal_title：Applied and environmental microbiology

authors： Musovic S,Oregaard G,Kroer N,Sørensen SJ

更新日期：2006-10-01 00:00:00

abstract：:Three bacteria capable of utilizing bis(4-hydroxyphenyl)methane (bisphenol F [BPF]) as the sole carbon source were isolated from river water, and they all belonged to the family Sphingomonadaceae. One of the isolates, designated Sphingobium yanoikuyae strain FM-2, at an initial cell density of 0.01 (optical density at...

journal_title：Applied and environmental microbiology

authors： Inoue D,Hara S,Kashihara M,Murai Y,Danzl E,Sei K,Tsunoi S,Fujita M,Ike M

更新日期：2008-01-01 00:00:00

abstract：:Light-dependent regulation of cell cycle progression in the marine cyanobacterium Synechococcus strain WH-8101 was demonstrated through the use of flow cytometry. Our results show that, similar to eucaryotic cells, marine Synechococcus spp. display two gaps in DNA synthesis, at the beginning and at the end of the cell...

journal_title：Applied and environmental microbiology

authors： Armbrust EV,Bowen JD,Olson RJ,Chisholm SW

更新日期：1989-02-01 00:00:00

abstract：:Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biol...

journal_title：Applied and environmental microbiology

authors： Liu XD,Wei Y,Zhou XY,Pei X,Zhang SH

更新日期：2015-10-01 00:00:00

abstract：:A real-time PCR assay was developed for the quantitative detection of Campylobacter jejuni in foods after enrichment culture. The specificity of the assay for C. jejuni was demonstrated with a diverse range of Campylobacter species, related organisms, and unrelated genera. The assay had a linear range of quantificatio...

journal_title：Applied and environmental microbiology

authors： Sails AD,Fox AJ,Bolton FJ,Wareing DR,Greenway DL

更新日期：2003-03-01 00:00:00

abstract：:Helicobacter pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers. Moreover, 50% of the population carries this bacterium, and consequently, when it is detected, eradication of H. pylori is strongly recommended. Regarding the use of probiotics as fu...

journal_title：Applied and environmental microbiology

authors： Chenoll E,Casinos B,Bataller E,Astals P,Echevarría J,Iglesias JR,Balbarie P,Ramón D,Genovés S

更新日期：2011-02-01 00:00:00

abstract：:A field study was conducted to determine the microbial community structures of streambed sediments across diverse geographic and climatic areas. Sediment samples were collected from three adjacent headwater forest streams within three biomes, eastern deciduous (Pennsylvania), southeastern coniferous (New Jersey), and ...

journal_title：Applied and environmental microbiology

authors： Findlay RH,Yeates C,Hullar MA,Stahl DA,Kaplan LA

更新日期：2008-05-01 00:00:00

abstract：:Microbial transformation of the antimelanoma agent betulinic acid was studied. The main objective of this study was to utilize microorganisms as in vitro models to predict and prepare potential mammalian metabolites of this compound. Preparative-scale biotransformation with resting-cell suspensions of Bacillus megater...

journal_title：Applied and environmental microbiology

authors： Chatterjee P,Kouzi SA,Pezzuto JM,Hamann MT

更新日期：2000-09-01 00:00:00

abstract：:A field collected population of Plutella xylostella (SERD4) was selected in the laboratory with Bacillus thuringiensis endotoxins Cry1Ac (Cry1Ac-SEL) and Cry1Ab (Cry1Ab-SEL). Both subpopulations showed similar phenotypes: high resistance to the Cry1A toxins and little cross-resistance to Cry1Ca or Cry1D. A previous an...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Ibiza-Palacios MS,Escriche B,Wright DJ,Crickmore N

更新日期：2005-11-01 00:00:00

abstract：:A nonmotile, gram-positive, spherical organism was isolated from the intestinal tracts of rabbits. Both hydrogen and methanol were required for growth. No methane was produced from hydrogen-carbon dioxide, formate, acetate, methylamines, ethanol, or isopropanol. The optimum pH was 6.8, and the optimum temperature was ...

journal_title：Applied and environmental microbiology

authors： Biavati B,Vasta M,Ferry JG

更新日期：1988-03-01 00:00:00

abstract：:Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

journal_title：Applied and environmental microbiology

authors： Klinke S,Dauner M,Scott G,Kessler B,Witholt B

更新日期：2000-03-01 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00

abstract：:The biological utilization of CO(2) and H(2) for the formation of short-chain fatty acids was studied by using a mixed culture of bacteria. Optimization of a medium was carried out in continuous culture to identify limiting factors which controlled growth and production of organic acids. The optimal pH for growth and ...

journal_title：Applied and environmental microbiology

authors： Goldberg I,Cooney CL

更新日期：1981-01-01 00:00:00

abstract：:Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article,...

journal_title：Applied and environmental microbiology

authors： Powell RJ,Hill RT

更新日期：2013-10-01 00:00:00