Identification of genes affecting hydrogen sulfide formation in Saccharomyces cerevisiae.

abstract：:Protoplasts of the monokaryotic strain 52J of Trametes versicolor were treated with UV light and screened for the inability to produce a colored precipitate on guaiacol-containing agar plates. Mutants unable to oxidize guaiacol had absent or very low secretion of laccase and manganese peroxidase (MnP) proteins. All is...

journal_title：Applied and environmental microbiology

authors： Addleman K,Dumonceaux T,Paice MG,Bourbonnais R,Archibald FS

更新日期：1995-10-01 00:00:00

abstract：:The N-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent I) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. The study included concentration, contact time, pH, temperature, and water quality as controlled variables. The species of bacteria teste...

journal_title：Applied and environmental microbiology

authors： Williams DE,Worley SD,Wheatley WB,Swango LJ

更新日期：1985-03-01 00:00:00

abstract：:Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

journal_title：Applied and environmental microbiology

authors： Geng T,Morgan MT,Bhunia AK

更新日期：2004-10-01 00:00:00

abstract：:The kinetics of adhesion of Vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. A modified Langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (Vibrio alginolyticus DP1HE4 and Vibrio anguillarum-like DC12R8 and DC12R9) showed a...

journal_title：Applied and environmental microbiology

authors： Bordas MA,Balebona MC,Zorrilla I,Borrego JJ,Moriñigo MA

更新日期：1996-10-01 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...

journal_title：Applied and environmental microbiology

authors： Gupta KR,Kasetty S,Chatterji D

更新日期：2015-04-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

journal_title：Applied and environmental microbiology

authors： Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

更新日期：2012-08-01 00:00:00

abstract：:Feeding of bovine milk to mice induced a high incidence of bacterial translocation from the intestines to the mesenteric lymph nodes, and the bacteria involved were mainly members of the family Enterobacteriaceae. Supplementation of the milk diet with bovine lactoferrin or a pepsin-generated hydrolysate of bovine lact...

journal_title：Applied and environmental microbiology

authors： Teraguchi S,Shin K,Ogata T,Kingaku M,Kaino A,Miyauchi H,Fukuwatari Y,Shimamura S

更新日期：1995-11-01 00:00:00

abstract：:Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques, in addition to the measurement of chemical profiles. After PCR-assisted sequence retrieval of the 16S rRNA genes (16S rDNA) from...

journal_title：Applied and environmental microbiology

authors： Zepp Falz K,Holliger C,Grosskopf R,Liesack W,Nozhevnikova AN,Müller B,Wehrli B,Hahn D

更新日期：1999-06-01 00:00:00

abstract：:The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

journal_title：Applied and environmental microbiology

authors： Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

更新日期：2010-11-01 00:00:00

abstract：:This is the first report on the fate of enterohemorrhagic Escherichia coli O157:H7 in simulated human colonic conditions. The pathogen was progressively eliminated from the bioreactor and did not modify the major populations of resident microbiota. The coadministration of the Saccharomyces cerevisiae CNCM I-3856 probi...

journal_title：Applied and environmental microbiology

authors： Thévenot J,Etienne-Mesmin L,Denis S,Chalancon S,Alric M,Livrelli V,Blanquet-Diot S

更新日期：2013-02-01 00:00:00

abstract：:For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, hea...

journal_title：Applied and environmental microbiology

authors： Lorenz H,Jäger C,Willems H,Baljer G

更新日期：1998-11-01 00:00:00

abstract：:A set of 22 phages of Listeria species (listeriaphages) (21 temperate and 1 virulent) were compared on the basis of morphology and protein composition. All 22 phages had icosahedral heads and exhibited either contractile or noncontractile tails. They represented two different morphotypes. Twenty phages belonged to the...

journal_title：Applied and environmental microbiology

authors： Zink R,Loessner MJ

更新日期：1992-01-01 00:00:00

abstract：:Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium ...

journal_title：Applied and environmental microbiology

authors： Muñoz G,Sotomayor P

更新日期：1990-07-01 00:00:00

abstract：:Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

journal_title：Applied and environmental microbiology

authors： Kobayashi H,Yoshida T,Inouye M

更新日期：2009-08-01 00:00:00

abstract：:Searles Lake occupies a closed basin harboring salt-saturated, alkaline brines that have exceptionally high concentrations of arsenic oxyanions. Strain SLAS-1(T) was previously isolated from Searles Lake (R. S. Oremland, T. R. Kulp, J. Switzer Blum, S. E. Hoeft, S. Baesman, L. G. Miller, and J. F. Stolz, Science 308:1...

journal_title：Applied and environmental microbiology

authors： Blum JS,Han S,Lanoil B,Saltikov C,Witte B,Tabita FR,Langley S,Beveridge TJ,Jahnke L,Oremland RS

更新日期：2009-04-01 00:00:00

abstract：:To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

journal_title：Applied and environmental microbiology

authors： Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

更新日期：1980-01-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:Recent outbreaks of food-borne illness associated with the consumption of produce have increased concern over wildlife reservoirs of food-borne pathogens. Wild rodents are ubiquitous, and those living close to agricultural farms may pose a food safety risk should they shed zoonotic microorganisms in their feces near o...

journal_title：Applied and environmental microbiology

authors： Kilonzo C,Li X,Vivas EJ,Jay-Russell MT,Fernandez KL,Atwill ER

更新日期：2013-10-01 00:00:00

abstract：:Rhodococcus erythropolis HL 24-1 isolated as a 2,4-dinitrophenol-degrading organism can utilize 2-chloro-4,6-dinitrophenol as the sole nitrogen, carbon, and energy source under aerobic conditions. This compound is metabolized with liberation of stoichiometric amounts of chloride and nitrite. Under anaerobic conditions...

journal_title：Applied and environmental microbiology

authors： Lenke H,Knackmuss H

更新日期：1996-03-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00

abstract：:Homologous recombination plays an important role in the structuring of genetic variation of many bacteria; however, its importance in adaptive evolution is not well established. We investigated the association of intersubspecific homologous recombination (IHR) with the shift to a novel host (mulberry) by the plant-pat...

journal_title：Applied and environmental microbiology

authors： Nunney L,Schuenzel EL,Scally M,Bromley RE,Stouthamer R

更新日期：2014-05-01 00:00:00

abstract：:A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...

journal_title：Applied and environmental microbiology

authors： Reddy CA,Henderson HE,Erdman MD

更新日期：1976-12-01 00:00:00

abstract：:Total extracted DNA from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. This was done by PCR amplification and cloning of a DNA segment encoding the active site of the enzyme. The translated sequences obtained fell into three similarity clu...

journal_title：Applied and environmental microbiology

authors： Standfuss-Gabisch C,Al-Halbouni D,Hofer B

更新日期：2012-04-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...

journal_title：Applied and environmental microbiology

authors： Villena I,Aubert D,Gomis P,Ferté H,Inglard JC,Denis-Bisiaux H,Dondon JM,Pisano E,Ortis N,Pinon JM

更新日期：2004-07-01 00:00:00

abstract：:Swarming Proteus spp. were detected with the use of proteometry (a most-probable-number technique) in the fecal material of selected animal species and in raw sewage from a local sewage treatment plant. Proteus spp. were not detected in any of several soil and freshwater samples examined. Since rat feces harbored high...

journal_title：Applied and environmental microbiology

authors： Stahl ML,Williams FD

更新日期：1981-03-01 00:00:00

abstract：:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (...

journal_title：Applied and environmental microbiology

authors： Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM

更新日期：1996-01-01 00:00:00

abstract：:Rates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73 °C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the...

journal_title：Applied and environmental microbiology

authors： Urschel MR,Kubo MD,Hoehler TM,Peters JW,Boyd ES

更新日期：2015-06-01 00:00:00

abstract：:Bottlenecks in protein expression and secretion often limit the development of industrial processes. By manipulating chaperone and foldase levels, improvements in yeast secretion were found for a number of proteins. Recently, sustained endoplasmic reticulum stress, occurring due to recombinant protein production, was ...

journal_title：Applied and environmental microbiology

authors： Raimondi S,Zanni E,Talora C,Rossi M,Palleschi C,Uccelletti D

更新日期：2008-12-01 00:00:00