Classification of virulent and temperate bacteriophages of Listeria spp. on the basis of morphology and protein analysis.

abstract：:Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identificat...

journal_title：Applied and environmental microbiology

authors： Valentine N,Wunschel S,Wunschel D,Petersen C,Wahl K

更新日期：2005-01-01 00:00:00

abstract：:To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

journal_title：Applied and environmental microbiology

authors： Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

更新日期：2014-12-01 00:00:00

abstract：:Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. The objective of this study was to characterize a collection of 437 Salmonella enterica isolates from different ani...

journal_title：Applied and environmental microbiology

authors： Lindsey RL,Fedorka-Cray PJ,Frye JG,Meinersmann RJ

更新日期：2009-04-01 00:00:00

abstract：:An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 20...

journal_title：Applied and environmental microbiology

authors： Anglès d'Auriac MB,Roberts H,Shaw T,Sirevåg R,Hermansen LF,Berg JD

更新日期：2000-10-01 00:00:00

abstract：:A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previou...

journal_title：Applied and environmental microbiology

authors： Delécluse A,Rosso ML,Ragni A

更新日期：1995-12-01 00:00:00

abstract：:A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph Methylomonas methanica on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was ...

journal_title：Applied and environmental microbiology

authors： Koh SC,Bowman JP,Sayler GS

更新日期：1993-04-01 00:00:00

abstract：:A gene fragment belonging to the cytochrome P-450 superfamily has been cloned and identified from stationary cultures of the filamentous fungus Phanerochaete chrysosporium by reverse transcriptase (RT)-PCR. A set of degenerate primers homologous to highly conserved regions of known cytochrome P-450 sequences were used...

journal_title：Applied and environmental microbiology

authors： Kullman SW,Matsumura F

更新日期：1997-07-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00

abstract：:Comparative genomic hybridizations have been used to examine genetic relationships among bacteria. The microarrays used in these experiments may have open reading frames from one or more reference strains (whole-genome microarrays), or they may be composed of random DNA fragments from a large number of strains (mixed-...

journal_title：Applied and environmental microbiology

authors： Wan Y,Broschat SL,Call DR

更新日期：2007-03-01 00:00:00

abstract：:Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

journal_title：Applied and environmental microbiology

authors： He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

更新日期：2012-07-01 00:00:00

abstract：:We investigated the effects of bottle enclosure on autotrophic and heterotrophic picoplankton in North and South subtropical Atlantic oligotrophic waters, where the biomass and metabolism of the microbial community are dominated by the picoplankton size class. We measured changes in both autotrophic (Prochlorococcus, ...

journal_title：Applied and environmental microbiology

authors： Calvo-Díaz A,Díaz-Pérez L,Suárez LÁ,Morán XA,Teira E,Marañón E

更新日期：2011-08-15 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

journal_title：Applied and environmental microbiology

authors： Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

更新日期：2016-05-16 00:00:00

abstract：:In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S ...

journal_title：Applied and environmental microbiology

authors： Duranti S,Turroni F,Milani C,Foroni E,Bottacini F,Dal Bello F,Ferrarini A,Delledonne M,van Sinderen D,Ventura M

更新日期：2013-01-01 00:00:00

abstract：:This study aimed at investigating the antifungal activity of Wickerhamomyces anomalus and sourdough lactic acid bacteria to extend the shelf life of wheat flour bread. The antifungal activity was assayed by agar diffusion, growth rate inhibition, and conidial germination assays, using Penicillium roqueforti DPPMAF1 as...

journal_title：Applied and environmental microbiology

authors： Coda R,Cassone A,Rizzello CG,Nionelli L,Cardinali G,Gobbetti M

更新日期：2011-05-01 00:00:00

abstract：:The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when ...

journal_title：Applied and environmental microbiology

authors： Fujito BT,Lytle CD

更新日期：1996-09-01 00:00:00

abstract：:The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was pre...

journal_title：Applied and environmental microbiology

authors： Kainulainen V,Reunanen J,Hiippala K,Guglielmetti S,Vesterlund S,Palva A,Satokari R

更新日期：2013-11-01 00:00:00

abstract：:A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H(2)S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H(2)S in a synthetic juice mediu...

journal_title：Applied and environmental microbiology

authors： Linderholm AL,Findleton CL,Kumar G,Hong Y,Bisson LF

更新日期：2008-03-01 00:00:00

abstract：:Experimental infection of animals with aerosols containing pathogenic agents is essential for an understanding of the natural history and pathogenesis of infectious disease as well as evaluation of potential treatments. We evaluated whether the Aeroneb nebulizer, a vibrating mesh nebulizer, would serve as an alternati...

journal_title：Applied and environmental microbiology

authors： Bowling JD,O'Malley KJ,Klimstra WB,Hartman AL,Reed DS

更新日期：2019-08-14 00:00:00

abstract：:The continuous addition of toluene as a solute of treated ballast water from oil tankers into a well-defined estuary facilitated the study of the dynamics of dissolved hydrocarbon metabolism in seawater. Most rates of toluene oxidation were in the range of 1 to 30 pg/liter per h at 0.5 mug of toluene per liter. Near t...

journal_title：Applied and environmental microbiology

authors： Button DK,Robertson BR,Craig KS

更新日期：1981-10-01 00:00:00

abstract：:Treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-Cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. Hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of wh...

journal_title：Applied and environmental microbiology

authors： Ragab-Depre NJ

更新日期：1982-09-01 00:00:00

abstract：:Subterranean clover plants possessing two equally infectible and robust lateral root systems ("split roots") were used in conjunction with several specific mutant strains (derived from Rhizobium trifolii ANU843) to investigate a systemic plant response induced by infective Rhizobium strains. This plant response contro...

journal_title：Applied and environmental microbiology

authors： Sargent L,Huang SZ,Rolfe BG,Djordjevic MA

更新日期：1987-07-01 00:00:00

abstract：:This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data ...

journal_title：Applied and environmental microbiology

authors： Awerbuch TE,Stark AA

更新日期：1979-12-01 00:00:00

abstract：:Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

journal_title：Applied and environmental microbiology

authors： McGowan C,Fulthorpe R,Wright A,Tiedje JM

更新日期：1998-10-01 00:00:00

abstract：:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as lon...

journal_title：Applied and environmental microbiology

authors： Whitby GE,Murray RG

更新日期：1980-01-01 00:00:00

abstract：:Filamentous, gliding, sulfide-oxidizing bacteria of the genus Thioploca were found on sediments in profundal areas of Lake Biwa, a Japanese freshwater mesotrophic lake, and were characterized morphologically and phylogenetically. The Lake Biwa Thioploca resembled morphologically Thioploca ingrica, a brackish water spe...

journal_title：Applied and environmental microbiology

authors： Kojima H,Teske A,Fukui M

更新日期：2003-01-01 00:00:00

abstract：:Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

journal_title：Applied and environmental microbiology

authors： Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

更新日期：2021-01-15 00:00:00

abstract：:Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

journal_title：Applied and environmental microbiology

authors： Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

更新日期：2013-09-01 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:The strictly anaerobic pathogenic bacterium Clostridium difficile occurs in the human gut and is able to thrive from fermentation of leucine. Thereby the amino acid is both oxidized to isovalerate plus CO(2) and reduced to isocaproate. In the reductive branch of this pathway, the dehydration of (R)-2-hydroxyisocaproyl...

journal_title：Applied and environmental microbiology

authors： Kim J,Darley D,Selmer T,Buckel W

更新日期：2006-09-01 00:00:00