Elution of viruses by ionic and nonionic surfactants.

abstract：:The human enteropathogen Clostridium difficile constitutes a key public health issue in industrialized countries. Many aspects of C. difficile pathophysiology and adaptation inside the host remain poorly understood. We have recently reported that this bacterium possesses an active CRISPR-Cas system of subtype I-B for ...

journal_title：Applied and environmental microbiology

authors： Maikova A,Kreis V,Boutserin A,Severinov K,Soutourina O

更新日期：2019-10-01 00:00:00

abstract：:A high-throughput method which is applicable for rapid screening, identification, and delineation of isolates of Vibrio cholerae, sensitive to genome variation, and capable of providing phylogenetic inferences enhances environmental monitoring of this bacterium. We have developed and optimized a method for genomic fin...

journal_title：Applied and environmental microbiology

authors： Chokesajjawatee N,Zo YG,Colwell RR

更新日期：2008-09-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00

abstract：:Adherence to hydroxyapatite (HA) was examined as a method to concentrate bacteria from foods. Using HA at a level of 10% and suspensions of an Escherichia coli strain containing 10(9), 10(6), and 10(3) cells per ml, kinetic studies revealed that maximum adherence was attained within 5 min for all cell concentrations a...

journal_title：Applied and environmental microbiology

authors： Berry ED,Siragusa GR

更新日期：1997-10-01 00:00:00

abstract：:Several cell surface characteristics of bacteriophage-resistant Lactococcus lactis subsp. cremoris SK110 were compared with those of its phage-sensitive derivative SK112. After centrifugation, SK110 cells resisted suspension more strongly than SK112 cells. SK112 was more negatively charged and had a more hydrophobic c...

journal_title：Applied and environmental microbiology

authors： Sijtsma L,Jansen N,Hazeleger WC,Wouters JT,Hellingwerf KJ

更新日期：1990-10-01 00:00:00

abstract：:Arctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first ...

journal_title：Applied and environmental microbiology

authors： Stevenson TJ,Buck CL,Duddleston KN

更新日期：2014-07-01 00:00:00

abstract：:Escherichia coli O157:H7 strains can be classified into different genotypes based on the presence of specific Shiga toxin-encoding bacteriophage insertion sites. Certain O157:H7 genotypes predominate among human clinical cases (clinical genotypes), while others are more frequently found in bovines (bovine-biased genot...

journal_title：Applied and environmental microbiology

authors： Vanaja SK,Springman AC,Besser TE,Whittam TS,Manning SD

更新日期：2010-01-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...

journal_title：Applied and environmental microbiology

authors： Limsawat S,Ohgaki S

更新日期：1997-07-01 00:00:00

abstract：:A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but...

journal_title：Applied and environmental microbiology

authors： Yan K,Dickman MB

更新日期：1996-08-01 00:00:00

abstract：:Four hitherto undescribed Clostridium strains capable of cleaving the C ring of quercetin, kaempferol, and naringenin at C-3-C-4 were isolated from the fecal flora of humans. None of the strains cleaved catechin. C-ring fission occurred when the substrate was either in solution or in suspension. Mixed cultures of flav...

journal_title：Applied and environmental microbiology

authors： Winter J,Moore LH,Dowell VR Jr,Bokkenheuser VD

更新日期：1989-05-01 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:The distribution of Clostridium botulinum serotypes A, B, E, and F in Finnish trout farms was examined. A total of 333 samples were tested with a neurotoxin-specific PCR assay. C. botulinum type E was found in 68% of the farm sediment samples, in 15% of the fish intestinal samples, and in 5% of the fish skin samples. ...

journal_title：Applied and environmental microbiology

authors： Hielm S,Björkroth J,Hyytiä E,Korkeala H

更新日期：1998-11-01 00:00:00

abstract：:To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) deco...

journal_title：Applied and environmental microbiology

authors： Pel R,Oldenhuis R,Brand W,Vos A,Gottschal JC,Zwart KB

更新日期：1997-02-01 00:00:00

abstract：:Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fe...

journal_title：Applied and environmental microbiology

authors： Fein JE,Zawadzki BC,Lawford HG,Lawford GR

更新日期：1983-06-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:Resistance to HIV infection in a cohort of commercial sex workers living in Nairobi, Kenya, is linked to mucosal and antiinflammatory factors that may be influenced by the vaginal microbiota. Since bacterial vaginosis (BV), a polymicrobial dysbiosis characterized by low levels of protective Lactobacillus organisms, is...

journal_title：Applied and environmental microbiology

authors： Schellenberg JJ,Links MG,Hill JE,Dumonceaux TJ,Kimani J,Jaoko W,Wachihi C,Mungai JN,Peters GA,Tyler S,Graham M,Severini A,Fowke KR,Ball TB,Plummer FA

更新日期：2011-06-01 00:00:00

abstract：:A soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. By standard bacteriological methods, this bacterium was ...

journal_title：Applied and environmental microbiology

authors： Mueller JG,Chapman PJ,Blattmann BO,Pritchard PH

更新日期：1990-04-01 00:00:00

abstract：:Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

journal_title：Applied and environmental microbiology

authors： Kretschmer A,Bock H,Wagner F

更新日期：1982-10-01 00:00:00

abstract：:A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial spe...

journal_title：Applied and environmental microbiology

authors： Ventura M,Fitzgerald GF,van Sinderen D

更新日期：2005-10-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat a...

journal_title：Applied and environmental microbiology

authors： Balandyté L,Brodard I,Frey J,Oevermann A,Abril C

更新日期：2011-12-01 00:00:00

abstract：:Acropora and Porites corals are important reef builders in the Indo-Pacific and Caribbean. Bacteria associated with mucus produced by Porites spp. and Acropora spp. from Caribbean (Punta Maroma, Mexico) and Indo-Pacific (Hoga and Sampela, Indonesia) reefs were determined. Analysis of pyrosequencing libraries showed th...

journal_title：Applied and environmental microbiology

authors： McKew BA,Dumbrell AJ,Daud SD,Hepburn L,Thorpe E,Mogensen L,Whitby C

更新日期：2012-08-01 00:00:00

abstract：:We studied the effects of a bacterium (Pseudomonas chlororaphis) and a bactivorous protozoan (Uronema sp.) on transformations of labile dissolved organic carbon (DOC). In 36-day time series experiments, bacteria were grown on glucose both with and without protozoa. We measured bulk organic carbon pools and used electr...

journal_title：Applied and environmental microbiology

authors： Gruber DF,Simjouw JP,Seitzinger SP,Taghon GL

更新日期：2006-06-01 00:00:00

abstract：:Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation ...

journal_title：Applied and environmental microbiology

authors： Hausner M,Wuertz S

更新日期：1999-08-01 00:00:00

abstract：:The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported. They were obtained by directly sequencing overlapping amplified fragments of the nuclear genes coding for the small subunit rRNA. These sequences were used to develop a polymerase chain reaction primer (VANS1) that enables the specifi...

journal_title：Applied and environmental microbiology

authors： Simon L,Lalonde M,Bruns TD

更新日期：1992-01-01 00:00:00

abstract：:Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the ...

journal_title：Applied and environmental microbiology

authors： Lin X,Kennedy D,Peacock A,McKinley J,Resch CT,Fredrickson J,Konopka A

更新日期：2012-02-01 00:00:00

abstract：:Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosa...

journal_title：Applied and environmental microbiology

authors： Pelletier A,Sygusch J

更新日期：1990-04-01 00:00:00

abstract：:A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...

journal_title：Applied and environmental microbiology

authors： François A,Mathis H,Godefroy D,Piveteau P,Fayolle F,Monot F

更新日期：2002-06-01 00:00:00

abstract：:Despite the increased number of anthrax outbreaks in Georgia and the other Caucasian republics of the former Soviet Union, no data are available on the diversity of the Bacillus anthracis strains involved. There is also little data available on strains from the former Soviet Union, including the strains previously use...

journal_title：Applied and environmental microbiology

authors： Merabishvili M,Natidze M,Rigvava S,Brusetti L,Raddadi N,Borin S,Chanishvili N,Tediashvili M,Sharp R,Barbeschi M,Visca P,Daffonchio D

更新日期：2006-08-01 00:00:00