Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.

abstract：:Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

journal_title：Applied and environmental microbiology

authors： Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

更新日期：2001-09-01 00:00:00

abstract：:We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coa...

journal_title：Applied and environmental microbiology

authors： Kitajima M,Oka T,Haramoto E,Katayama H,Takeda N,Katayama K,Ohgaki S

更新日期：2010-04-01 00:00:00

abstract：:Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase)...

journal_title：Applied and environmental microbiology

authors： Johansson B,Christensson C,Hobley T,Hahn-Hägerdal B

更新日期：2001-09-01 00:00:00

abstract：:The enrichment and isolation of thermophilic bacteria capable of rubber [poly(cis-1,4-isoprene)] degradation revealed eight different strains exhibiting both currently known strategies used by rubber-degrading mesophilic bacteria. Taxonomic characterization of these isolates by 16S rRNA gene sequence analysis demonstr...

journal_title：Applied and environmental microbiology

authors： Ibrahim EM,Arenskötter M,Luftmann H,Steinbüchel A

更新日期：2006-05-01 00:00:00

abstract：:The promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was used to drive expression of mnp1, the gene encoding Mn peroxidase isozyme 1, in primary metabolic cultures of Phanerochaete chrysosporium. A 1,100-bp fragment of the P. chrysosporium gpd promoter region was fused upstream of the mnp1 ge...

journal_title：Applied and environmental microbiology

authors： Mayfield MB,Kishi K,Alic M,Gold MH

更新日期：1994-12-01 00:00:00

abstract：:Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

journal_title：Applied and environmental microbiology

authors： Bradley PM,Richmond S,Chapelle FH

更新日期：2005-10-01 00:00:00

abstract：:Ursodeoxycholic acid dissolves cholesterol gallstones in humans. In the present study optimum conditions for ursodeoxycholic acid production by Fusarium equiseti M41 were studied. Resting mycelia of F. equiseti M41 showed maximum conversion at 28 degrees C, pH 8.0, and dissolved oxygen tension of higher than 60% satur...

journal_title：Applied and environmental microbiology

authors： Kulprecha S,Ueda T,Nihira T,Yoshida T,Taguchi H

更新日期：1985-02-01 00:00:00

abstract：:Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

journal_title：Applied and environmental microbiology

authors： Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

更新日期：2010-04-01 00:00:00

abstract：:A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dis...

journal_title：Applied and environmental microbiology

authors： Francoeur SN,Wetzel RG,Neely RK

更新日期：2001-09-01 00:00:00

abstract：:Application of a mycorrhizal inoculum could be one way to increase the yield of rice plants and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading, and ripening stages in four paddy w...

journal_title：Applied and environmental microbiology

authors： Wang Y,Li T,Li Y,Björn LO,Rosendahl S,Olsson PA,Li S,Fu X

更新日期：2015-04-01 00:00:00

abstract：:The role of three key nitrogen regulatory genes, glnB (encoding the P(II) protein), glnZ (encoding the P(z) protein), and glnD (encoding the GlnD protein), in regulation of poly-3-hydroxybutyrate (PHB) biosynthesis by ammonia in Azospirillum brasilense Sp7 was investigated. It was observed that glnB glnZ and glnD muta...

journal_title：Applied and environmental microbiology

authors： Sun J,Van Dommelen A,Van Impe J,Vanderleyden J

更新日期：2002-02-01 00:00:00

abstract：:Vibrio vulnificus naturally inhabits a variety of aquatic organisms, including oysters, and is the leading cause of seafood-related death in the United States. Strains of this bacterium are genetically classified into environmental (E) and clinical (C) genotypes, which correlate with source of isolation. E-genotype st...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2014-03-01 00:00:00

abstract：:Laccase is a copper-containing phenoloxidase, involved in lignin degradation by white rot fungi. The laccase substrate range can be extended to include nonphenolic lignin subunits in the presence of a noncatalytic cooxidant such as 2, 2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), with ABTS being oxidized to t...

journal_title：Applied and environmental microbiology

authors： Collins PJ,Dobson ADW,Field JA

更新日期：1998-06-01 00:00:00

abstract：:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (b...

journal_title：Applied and environmental microbiology

authors： Ceragioli M,Mols M,Moezelaar R,Ghelardi E,Senesi S,Abee T

更新日期：2010-05-01 00:00:00

abstract：:Escherichia coli ATCC 12806 was exposed to increasing subinhibitory concentrations of three biocides widely used in food industry facilities: trisodium phosphate (TSP), sodium nitrite (SNI), and sodium hypochlorite (SHY). The cultures exhibited an acquired tolerance to biocides (especially to SNI and SHY) after exposu...

journal_title：Applied and environmental microbiology

authors： Capita R,Riesco-Peláez F,Alonso-Hernando A,Alonso-Calleja C

更新日期：2014-02-01 00:00:00

abstract：:An experiment was conducted with subsurface sediments from Oak Ridge National Laboratory to determine the potential for reduction of U(VI) under sulfate-reducing conditions with either ethanol or acetate as the electron donor. The results showed extensive U(VI) reduction in sediments supplied with either electron dono...

journal_title：Applied and environmental microbiology

authors： Converse BJ,Wu T,Findlay RH,Roden EE

更新日期：2013-07-01 00:00:00

abstract：:Although many Lactobacillus strains used as probiotics are believed to modulate host immune responses, the molecular natures of the components of such probiotic microorganisms directly involved in immune modulation process are largely unknown. We aimed to assess the function of polysaccharide moiety of the cell wall o...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Serata M,Sako T

更新日期：2008-08-01 00:00:00

abstract：:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive lux...

journal_title：Applied and environmental microbiology

authors： de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

更新日期：1991-12-01 00:00:00

abstract：:Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activit...

journal_title：Applied and environmental microbiology

authors： Mayer VW,Goin CJ

更新日期：1983-02-01 00:00:00

abstract：:The capacity of Azospirillum brasilense to enhance the accumulation of K, P, Ca, Mg, S, Na, Mn, Fe, B, Cu, and Zn in inoculated wheat and soybean plants was evaluated by using two different analytical methods with five A. brasilense strains originating from four distinct geographical regions. A Pseudomonas isolate fro...

journal_title：Applied and environmental microbiology

authors： Bashan Y,Harrison SK,Whitmoyer RE

更新日期：1990-03-01 00:00:00

abstract：:The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

journal_title：Applied and environmental microbiology

authors： McMeekin TA

更新日期：1977-06-01 00:00:00

abstract：:Stability of foreign DNA transformed into a novel host is an important parameter in decisions to permit the release of genetically engineered microorganisms into the environment. Meiotic instability of transformed DNA has been reported in fungi such as Ascobolus, Aspergillus, and Neurospora. We used strains of Gibbere...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Dickman MB

更新日期：1991-05-01 00:00:00

abstract：:Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

更新日期：2008-12-01 00:00:00

abstract：:A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and red...

journal_title：Applied and environmental microbiology

authors： Thimm T,Tebbe CC

更新日期：2003-05-01 00:00:00

abstract：:The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....

journal_title：Applied and environmental microbiology

authors： Murata H,Chatterjee A,Liu Y,Chatterjee AK

更新日期：1994-09-01 00:00:00

abstract：:A norleucine-resistant mutant was derived from an isoleucine-valine auxotroph of a leucine accumulator of Serratia marcescens. The norleucine-resistant mutant could accumulate norleucine from norvaline in the medium without the addition of methionine, which antagonized norleucine. This mutant constitutively formed hom...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Sugiura M,Chibata I

更新日期：1977-08-01 00:00:00

abstract：:Legionella pneumophila suspended in tap water was exposed to biocides recommended for inhibiting biological growth in cooling towers and evaporative condensers of air-conditioning systems. Chlorine, 2,2-dibromo-3-nitrilopropionamide, and a compound containing didecyldimethylammonium chloride and isopropanol were effec...

journal_title：Applied and environmental microbiology

authors： Skaliy P,Thompson TA,Gorman GW,Morris GK,McEachern HV,Mackel DC

更新日期：1980-10-01 00:00:00

abstract：:GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...

journal_title：Applied and environmental microbiology

authors： Lin CC,Casida LE

更新日期：1984-02-01 00:00:00

abstract：:Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relativ...

journal_title：Applied and environmental microbiology

authors： Król JE,Penrod JT,McCaslin H,Rogers LM,Yano H,Stancik AD,Dejonghe W,Brown CJ,Parales RE,Wuertz S,Top EM

更新日期：2012-02-01 00:00:00

abstract：:Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration ...

journal_title：Applied and environmental microbiology

authors： Sayyed AH,Gatsi R,Kouskoura T,Wright DJ,Crickmore N

更新日期：2001-09-01 00:00:00