Abstract:
:The role of three key nitrogen regulatory genes, glnB (encoding the P(II) protein), glnZ (encoding the P(z) protein), and glnD (encoding the GlnD protein), in regulation of poly-3-hydroxybutyrate (PHB) biosynthesis by ammonia in Azospirillum brasilense Sp7 was investigated. It was observed that glnB glnZ and glnD mutants produce substantially higher amounts of PHB than the wild type produces during the active growth phase. glnB and glnZ mutants have PHB production phenotypes similar to that of the wild type. Our results indicate that the P(II)-P(z) system is apparently involved in nitrogen-dependent regulation of PHB biosynthesis in A. brasilense Sp7.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Sun J,Van Dommelen A,Van Impe J,Vanderleyden Jdoi
10.1128/aem.68.2.985-988.2002keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
985-8issue
2eissn
0099-2240issn
1098-5336journal_volume
68pub_type
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