Abstract:
:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive luxAB construct (coding only for the luciferase) was introduced into the Pseudomonas cells, light emission could be initiated just prior to measurement by the addition of naphthalene or the substrate for luciferase, n-decyl aldehyde, respectively. These Pseudomonas cells could successfully be detected in the rhizosphere by using autophotography or optical fiber light measurement techniques. Detection required the presence of 10 to 10 CFU/cm of root, showing that the bioluminescence technique is at least 1,000-fold more sensitive than beta-galactosidase-based systems.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GSdoi
10.1128/AEM.57.12.3641-3644.1991keywords:
subject
Has Abstractpub_date
1991-12-01 00:00:00pages
3641-4issue
12eissn
0099-2240issn
1098-5336journal_volume
57pub_type
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