Use of Bioluminescence Markers To Detect Pseudomonas spp. in the Rhizosphere.

Abstract:

:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive luxAB construct (coding only for the luciferase) was introduced into the Pseudomonas cells, light emission could be initiated just prior to measurement by the addition of naphthalene or the substrate for luciferase, n-decyl aldehyde, respectively. These Pseudomonas cells could successfully be detected in the rhizosphere by using autophotography or optical fiber light measurement techniques. Detection required the presence of 10 to 10 CFU/cm of root, showing that the bioluminescence technique is at least 1,000-fold more sensitive than beta-galactosidase-based systems.

journal_name

Appl Environ Microbiol

authors

de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

doi

10.1128/AEM.57.12.3641-3644.1991

keywords:

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

3641-4

issue

12

eissn

0099-2240

issn

1098-5336

journal_volume

57

pub_type

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