Influence of CO(2)-HCO(3) Levels and pH on Growth, Succinate Production, and Enzyme Activities of Anaerobiospirillum succiniciproducens.

Abstract:

:Growth and succinate versus lactate production from glucose by Anaerobiospirillum succiniciproducens was regulated by the level of available carbon dioxide and culture pH. At pH 7.2, the generation time was almost doubled and extensive amounts of lactate were formed in comparison with growth at pH 6.2. The succinate yield and the yield of ATP per mole of glucose were significantly enhanced under excess-CO(2)-HCO(3) growth conditions and suggest that there exists a threshold level of CO(2) for enhanced succinate production in A. succiniciproducens. Glucose was metabolized via the Embden-Meyerhof-Parnas route, and phosphoenolpyruvate carboxykinase levels increased while lactate dehydrogenase and alcohol dehydrogenase levels decreased under excess-CO(2)-HCO(3) growth conditions. Kinetic analysis of succinate and lactate formation in continuous culture indicated that the growth rate-linked production rate coefficient (K) cells was much higher for succinate (7.2 versus 1.0 g/g of cells per h) while the non-growth-rate-related formation rate coefficient (K') was higher for lactate (1.1 versus 0.3 g/g of cells per h). The data indicate that A. succiniciproducens, unlike other succinate-producing anaerobes which also form propionate, can grow rapidly and form high final yields of succinate at pH 6.2 and with excess CO(2)-HCO(3) as a consequence of regulating electron sink metabolism.

journal_name

Appl Environ Microbiol

authors

Samuelov NS,Lamed R,Lowe S,Zeikus JG

doi

10.1128/AEM.57.10.3013-3019.1991

keywords:

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

3013-9

issue

10

eissn

0099-2240

issn

1098-5336

journal_volume

57

pub_type

杂志文章
  • Metatranscriptomic analysis of microbes in an Oceanfront deep-subsurface hot spring reveals novel small RNAs and type-specific tRNA degradation.

    abstract::Studies of small noncoding RNAs (sRNAs) have been conducted predominantly using culturable organisms, and the acquisition of further information about sRNAs from global environments containing uncultured organisms now is very important. In this study, hot spring water (57°C, pH 8.1) was collected directly from the und...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06811-11

    authors: Murakami S,Fujishima K,Tomita M,Kanai A

    更新日期:2012-02-01 00:00:00

  • Action of douglas fir tussock moth larvae and their microflora on dietary terpenes.

    abstract::A single type of bacterium, tentatively identified as a member of the genus Bacillus, was isolated from 2 of 20 midguts of Douglas fir tussock moth larvae being fed a diet of fir needles. No bacteria could be isolated from most midguts. Although spherically shaped bodies were present in the food bolus, these bodies, i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.5.959-963.1980

    authors: Andrews RE,Spence KD

    更新日期:1980-11-01 00:00:00

  • Aerobic, Selenium-Utilizing Bacillus Isolated from Seeds of Astragalus crotalariae.

    abstract::Bacillus sp. strain SS, an aerobic, gram-positive sporeformer, was isolated from seeds of Astragalus crotalariae, a selenium-accumulating plant. This bacillus grew in a nutrient broth (containing beef extract and peptone) if the medium was supplemented with high concentrations of selenium. Concentrations of Na(2)SeO(3...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.3.737-743.1982

    authors: Lindblow-Kull C,Shrift A,Gherna RL

    更新日期:1982-09-01 00:00:00

  • Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain ac10: gene cloning and enzyme purification and characterization.

    abstract::The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of 85,113. SapSh exhib...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.611-617.1999

    authors: Kulakova L,Galkin A,Kurihara T,Yoshimura T,Esaki N

    更新日期:1999-02-01 00:00:00

  • Shuttle vector expression in Thermococcus kodakaraensis: contributions of cis elements to protein synthesis in a hyperthermophilic archaeon.

    abstract::Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00305-08

    authors: Santangelo TJ,Cubonová L,Reeve JN

    更新日期:2008-05-01 00:00:00

  • Characterization of airborne bacteria at an underground subway station.

    abstract::The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07212-11

    authors: Dybwad M,Granum PE,Bruheim P,Blatny JM

    更新日期:2012-03-01 00:00:00

  • Protein degradation by ruminal microorganisms from sheep fed dietary supplements of urea, casein, or albumin.

    abstract::Ruminal fluid from sheep fed hay plus concentrate diets containing 1.8% urea, 6% casein, or 6% egg albumin had proteolytic activities of 4.12, 3.02, or 4.00 mg of [14C]casein hydrolyzed ml-1 h-1, respectively. Dietary albumin had no effect on the rate of albumin breakdown relative to that of casein (0.06). Greater num...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.4.751-753.1987

    authors: Wallace RJ,Broderick GA,Brammall ML

    更新日期:1987-04-01 00:00:00

  • Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons.

    abstract::Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4264-4267.1999

    authors: Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

    更新日期:1999-09-01 00:00:00

  • Rapid evaluation of the mycobactericidal efficacy of disinfectants in the quantitative carrier test EN 14563 by using fluorescent Mycobacterium terrae.

    abstract::To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01660-09

    authors: Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

    更新日期:2010-01-01 00:00:00

  • Marine cyanophages demonstrate biogeographic patterns throughout the global ocean.

    abstract::Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02483-14

    authors: Huang S,Zhang S,Jiao N,Chen F

    更新日期:2015-01-01 00:00:00

  • Prevalence of Ca²⁺-ATPase-mediated carbonate dissolution among cyanobacterial euendoliths.

    abstract::Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06633-11

    authors: Ramírez-Reinat EL,Garcia-Pichel F

    更新日期:2012-01-01 00:00:00

  • 3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species.

    abstract::Glycerol fermentation by Enterobacter agglomerans revealed that both growth and 1,3-propanediol production ceased after consumption of about 430 mM glycerol, irrespective of the initial glycerol content. This phenomenon was assigned to the production of 3-hydroxypropionaldehyde, which was identified by proton nuclear ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.4.1448-1451.1996

    authors: Barbirato F,Grivet JP,Soucaille P,Bories A

    更新日期:1996-04-01 00:00:00

  • Thiosulfate-dependent chemolithoautotrophic growth of Bradyrhizobium japonicum.

    abstract::Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02783-09

    authors: Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

    更新日期:2010-04-01 00:00:00

  • High-Level Nickel Resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2.

    abstract::Two new nickel-resistant strains of Alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. Alcaligenes xylosoxydans 31A is a heterotrophic bacterium, and Alcaligenes eutrophus KTO2 is an a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.11.3301-3309.1991

    authors: Schmidt T,Stoppel RD,Schlegel HG

    更新日期:1991-11-01 00:00:00

  • Relationships among Cell Size, Membrane Permeability, and Preservative Resistance in Yeast Species.

    abstract::The rate of uptake of propanoic acid and the cell dimensions were measured for 23 yeasts differing in their resistance to weak-acid-type preservatives. Relationships between reciprocal uptake rate, reciprocal permeability, cell volume, cell area, volume/area, and the MICs of benzoic acid and propanoic acid for the yea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.11.2995-2999.1989

    authors: Warth AD

    更新日期:1989-11-01 00:00:00

  • Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities.

    abstract::In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03384-16

    authors: Mao X,Polasko A,Alvarez-Cohen L

    更新日期:2017-03-31 00:00:00

  • Heat-induced temperature sensitivity of outgrowing Bacillus cereus spores.

    abstract::Inactivation of Bacillus cereus spores during cooling (10 degrees C/h) from 90 degrees C occurred in two phases. One phase occurred during cooling from 90 to 80 degrees C; the second occurred during cooling from 46 to 38 degrees C. In contrast, no inactivation occurred when spores were cooled from a maximum temperatur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.4.768-774.1984

    authors: Johnson KM,Busta FF

    更新日期:1984-04-01 00:00:00

  • New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli.

    abstract::Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03803-14

    authors: Schwendener S,Perreten V

    更新日期:2015-05-01 00:00:00

  • Novel probiotic Bifidobacterium bifidum CECT 7366 strain active against the pathogenic bacterium Helicobacter pylori.

    abstract::Helicobacter pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers. Moreover, 50% of the population carries this bacterium, and consequently, when it is detected, eradication of H. pylori is strongly recommended. Regarding the use of probiotics as fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01820-10

    authors: Chenoll E,Casinos B,Bataller E,Astals P,Echevarría J,Iglesias JR,Balbarie P,Ramón D,Genovés S

    更新日期:2011-02-01 00:00:00

  • Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis.

    abstract::In Europe, Borrelia burgdorferi genospecies causing Lyme borreliosis are mainly transmitted by the tick Ixodes ricinus. Since its discovery, B. burgdorferi has been the subject of many epidemiological studies to determine its prevalence and the distribution of the different genospecies in ticks. In the current study w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,meta分析

    doi:10.1128/AEM.71.11.7203-7216.2005

    authors: Rauter C,Hartung T

    更新日期:2005-11-01 00:00:00

  • Effect of rice plants on nitrogenase activity of flooded soils.

    abstract::In samples of flooded soil containing blue-green algae (cyanobacteria), the presence of rice plants did not influence the nitrogenase activity of the algae. Nitrogenase activity of heterotrophic bacteria was enhanced by the presence of rice plants, but this activity was not affected by changes in plant density. The ra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.3.507-510.1980

    authors: Habte M,Alexander M

    更新日期:1980-09-01 00:00:00

  • Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis.

    abstract::A soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. By standard bacteriological methods, this bacterium was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.1079-1086.1990

    authors: Mueller JG,Chapman PJ,Blattmann BO,Pritchard PH

    更新日期:1990-04-01 00:00:00

  • Determination of clonality and relatedness of Vibrio cholerae isolates by genomic fingerprinting, using long-range repetitive element sequence-based PCR.

    abstract::A high-throughput method which is applicable for rapid screening, identification, and delineation of isolates of Vibrio cholerae, sensitive to genome variation, and capable of providing phylogenetic inferences enhances environmental monitoring of this bacterium. We have developed and optimized a method for genomic fin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00151-08

    authors: Chokesajjawatee N,Zo YG,Colwell RR

    更新日期:2008-09-01 00:00:00

  • Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil.

    abstract::The root-colonizing bacterium Pseudomonas fluorescens CHA0 was used to construct an oxygen-responsive biosensor. An anaerobically inducible promoter of Pseudomonas aeruginosa, which depends on the FNR (fumarate and nitrate reductase regulation)-like transcriptional regulator ANR (anaerobic regulation of arginine deimi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4085-4093.1999

    authors: Højberg O,Schnider U,Winteler HV,Sørensen J,Haas D

    更新日期:1999-09-01 00:00:00

  • Characterization of an NaCl-sensitive Staphylococcus aureus mutant and rescue of the NaCl-sensitive phenotype by glycine betaine but not by other compatible solutes.

    abstract::To further study mechanisms of coping with osmotic stress-low water activity, mutants of Staphylococcus aureus with transposon Tn917-lacZ-induced NaCl sensitivity were selected for impaired ability to grow on solid defined medium containing 2 M NaCl. Southern hybridization experiments showed that NaCl-sensitive mutant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.5.1889-1897.1997

    authors: Vijaranakul U,Nadakavukaren MJ,Bayles DO,Wilkinson BJ,Jayaswal RK

    更新日期:1997-05-01 00:00:00

  • Oxidation of ethylene glycol by a salt-requiring bacterium.

    abstract::Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.1.180-183.1981

    authors: Caskey WH,Taber WA

    更新日期:1981-07-01 00:00:00

  • Metabolic and Genomic Traits of Phytobeneficial Phenazine-Producing Pseudomonas spp. Are Linked to Rhizosphere Colonization in Arabidopsis thaliana and Solanum tuberosum.

    abstract::Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing P...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02443-19

    authors: Zboralski A,Biessy A,Savoie MC,Novinscak A,Filion M

    更新日期:2020-02-03 00:00:00

  • Data Acceptance Criteria for Standardized Human-Associated Fecal Source Identification Quantitative Real-Time PCR Methods.

    abstract::There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03661-15

    authors: Shanks OC,Kelty CA,Oshiro R,Haugland RA,Madi T,Brooks L,Field KG,Sivaganesan M

    更新日期:2016-04-18 00:00:00

  • Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic.

    abstract::Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.3.1210-1214.2005

    authors: Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

    更新日期:2005-03-01 00:00:00

  • Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines.

    abstract::A novel S-adenosyl-L-methionine (SAM)-dependent methyltransferase catalyzing the O methylation of several chlorophenols and other halogenated phenols was purified 220-fold to apparent homogeneity from mycelia of Trichoderma longibrachiatum CECT 20431. The enzyme could be identified in partially purified protein prepar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.9.5089-5095.2003

    authors: Coque JJ,Alvarez-Rodríguez ML,Larriba G

    更新日期:2003-09-01 00:00:00