New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli.

abstract：:Environmental bacteria persist in various habitats, yet little is known about the genes that contribute to growth and survival in their respective ecological niches. Signature-tagged mutagenesis (STM) of Shewanella oneidensis MR-1 coupled with a screen involving incubations of mutant strains in anoxic aquifer sediment...

journal_title：Applied and environmental microbiology

authors： Groh JL,Luo Q,Ballard JD,Krumholz LR

更新日期：2007-01-01 00:00:00

abstract：:Sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. Based on their hydrolysis profile patterns, Lactobacillus alimentarius 15M, Lactobacillus brevis 14G, Lactobacillus sanfranciscensis 7A, and Lactobacillus hilgardii...

journal_title：Applied and environmental microbiology

authors： Di Cagno R,De Angelis M,Lavermicocca P,De Vincenzi M,Giovannini C,Faccia M,Gobbetti M

更新日期：2002-02-01 00:00:00

abstract：:Algal-virus-specific PCR primers were used to amplify DNA polymerase gene (pol) fragments (683 to 689 bp) from the virus-sized fraction (0.02 to 0.2 microns) concentrated from inshore and offshore water samples collected from the Gulf of Mexico. Algal-virus-like DNA pol genes were detected in five samples collected fr...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA,Short SM

更新日期：1996-08-01 00:00:00

abstract：:Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

journal_title：Applied and environmental microbiology

authors： Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

更新日期：2005-12-01 00:00:00

abstract：:The electron donor cytochrome b5 (CybE/Cyb5) fuels the activity of the ergosterol biosynthesis-related P450 enzymes/P450s by providing electrons to P450s to promote ergosterol biosynthesis. Previous studies reported that lack of Aspergillus fumigatus (A. fumigatus) CybE reduces the proportion of ergosterol in total st...

journal_title：Applied and environmental microbiology

authors： Zhang C,Ren Y,Gao L,Gu H,Lu L

更新日期：2020-11-30 00:00:00

abstract：:Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and pro...

journal_title：Applied and environmental microbiology

authors： Arensdorf JJ,Focht DD

更新日期：1995-02-01 00:00:00

abstract：:Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...

journal_title：Applied and environmental microbiology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1992-04-01 00:00:00

abstract：:We investigated associations of species of the Bacteroides fragilis group with Japanese cedar pollinosis (JCPsis). Cell numbers of Bacteroides fragilis and Bacteroides intestinalis were significantly higher in JCPsis subjects than in non-JCPsis subjects before the pollen season. They correlated positively with both sy...

journal_title：Applied and environmental microbiology

authors： Odamaki T,Xiao JZ,Sakamoto M,Kondo S,Yaeshima T,Iwatsuki K,Togashi H,Enomoto T,Benno Y

更新日期：2008-11-01 00:00:00

abstract：:Streptococcus thermophilus is a facultative anaerobic bacterium that has the ability to grow and survive in aerobic environments, but the mechanism for this remains unclear. In this study, the efeB gene, encoding a dye-decolorizing peroxidase, was identified in the genome of Streptococcus thermophilus CGMCC 7.179, and...

journal_title：Applied and environmental microbiology

authors： Zhang C,Xin Y,Wang Y,Guo T,Lu S,Kong J

更新日期：2015-09-01 00:00:00

abstract：:The haloalkane dehalogenase enzyme DmmA was identified by marine metagenomic screening. Determination of its crystal structure revealed an unusually large active site compared to those of previously characterized haloalkane dehalogenases. Here we present a biochemical characterization of this interesting enzyme with e...

journal_title：Applied and environmental microbiology

authors： Buryska T,Babkova P,Vavra O,Damborsky J,Prokop Z

更新日期：2018-01-02 00:00:00

abstract：:Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean...

journal_title：Applied and environmental microbiology

authors： Demezas DH,Reardon TB,Watson JM,Gibson AH

更新日期：1991-12-01 00:00:00

abstract：:The purpose of this investigation was to examine the capacity of the biphenyl catabolic enzymes of Comamonas testosteroni B-356 to metabolize dihydroxybiphenyls symmetrically substituted on both rings. Data show that 3,3'-dihydroxybiphenyl is by far the preferred substrate for strain B-356. However, the dihydrodiol me...

journal_title：Applied and environmental microbiology

authors： Sondossi M,Barriault D,Sylvestre M

更新日期：2004-01-01 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

journal_title：Applied and environmental microbiology

authors： Nakayama A,Yano Y,Yoshida K

更新日期：1994-11-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...

journal_title：Applied and environmental microbiology

authors： Wagh A,Shen S,Shen FA,Miller CD,Walsh MK

更新日期：2012-05-01 00:00:00

abstract：:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

journal_title：Applied and environmental microbiology

authors： Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

更新日期：1993-06-01 00:00:00

abstract：:An acclimated shake flask CO(2) evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO(2) evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured. With only a few exceptions, primary ...

journal_title：Applied and environmental microbiology

authors： Sugatt RH,O'grady DP,Banerjee S,Howard PH,Gledhill WE

更新日期：1984-04-01 00:00:00

abstract：:The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacterium Methylophilus methylotrophus AS1 that are auxotrophic for aromatic...

journal_title：Applied and environmental microbiology

authors： Yomantas YA,Tokmakova IL,Gorshkova NV,Abalakina EG,Kazakova SM,Gak ER,Mashko SV

更新日期：2010-01-01 00:00:00

abstract：:We previously showed a pivotal role of the polysaccharide (PS) moiety in the cell wall of the Lactobacillus casei strain Shirota (YIT 9029) as a possible immune modulator (E. Yasuda M. Serata, and T. Sako, Appl. Environ. Microbiol. 74:4746-4755, 2008). To distinguish PS structures on the bacterial cell surface of indi...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Tateno H,Hirabayashi J,Iino T,Sako T

更新日期：2011-07-01 00:00:00

abstract：:The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

journal_title：Applied and environmental microbiology

authors： Marciano-Cabral FM,Fulford DE

更新日期：1986-05-01 00:00:00

abstract：:A new bacterial pathogen of chinook salmon (oncorhynchus tshawytscha) was isolated from fish in Oregon's Rogue River. The bacteria are biochemically and serologically related to strains of Edwardsiella tarda. Initially isolated from chinook salmon, the bacteria were also pathogenic for steelhead and rainbow trout (Sal...

journal_title：Applied and environmental microbiology

authors： Amandi A,Hiu SF,Rohovec JS,Fryer JL

更新日期：1982-06-01 00:00:00

abstract：:Waters in marine and freshwater areas of Puerto Rico were analyzed for the presence of Legionella spp. by direct fluorescent antibody assay with guinea pig confirmation. Several species, including L. bozemanii, L. dumoffii, L. gormanii, L. longbeachae, L. micdadei, and L. pneumophila, were widely distributed among all...

journal_title：Applied and environmental microbiology

authors： Ortiz-Roque CM,Hazen TC

更新日期：1987-09-01 00:00:00

abstract：:The frequency of isolation of three nonhomologous chlorobenzoate catabolic genotypes (clc, cba, and fcb) was determined for 464 isolates from freshwater sediments and groundwater in the vicinity of the Hyde Park industrial landfill site in the Niagara watershed. Samples were collected from both contaminated and noncon...

journal_title：Applied and environmental microbiology

authors： Peel MC,Wyndham RC

更新日期：1999-04-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：:[This corrects the article on p. 2161 in vol. 59.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1993-12-01 00:00:00

abstract：:A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

journal_title：Applied and environmental microbiology

authors： Kennedy MJ,Lawless JG

更新日期：1985-01-01 00:00:00

abstract：:Chemolithotrophic ammonium-oxidizing and nitrite-oxidizing bacteria including Nitrosomonas europaea, Nitrosococcus oceanus, Nitrobacter sp., Nitiospina gracilis, and Nitrococcus mobilis were examined as to their ability to oxidize methane in the absence of ammonium or nitrite. All ammonium oxidizers tested had the abi...

journal_title：Applied and environmental microbiology

authors： Jones RD,Morita RY

更新日期：1983-02-01 00:00:00

abstract：:The enrichment and isolation of thermophilic bacteria capable of rubber [poly(cis-1,4-isoprene)] degradation revealed eight different strains exhibiting both currently known strategies used by rubber-degrading mesophilic bacteria. Taxonomic characterization of these isolates by 16S rRNA gene sequence analysis demonstr...

journal_title：Applied and environmental microbiology

authors： Ibrahim EM,Arenskötter M,Luftmann H,Steinbüchel A

更新日期：2006-05-01 00:00:00

abstract：:Pseudobactin production by Pseudomonas putida WCS358 significantly improves biological control of fusarium wilt caused by nonpathogenic Fusarium oxysporum Fo47b10 (P. Lemanceau, P. A. H. M. Bakker, W. J. de Kogel, C. Alabouvette, and B. Schippers, Appl. Environ. Microbiol. 58:2978-2982, 1992). The antagonistic effect ...

journal_title：Applied and environmental microbiology

authors： Lemanceau P,Bakker PA,De Kogel WJ,Alabouvette C,Schippers B

更新日期：1993-01-01 00:00:00