Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract.

abstract：:Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-...

journal_title：Applied and environmental microbiology

authors： Bretschger O,Obraztsova A,Sturm CA,Chang IS,Gorby YA,Reed SB,Culley DE,Reardon CL,Barua S,Romine MF,Zhou J,Beliaev AS,Bouhenni R,Saffarini D,Mansfeld F,Kim BH,Fredrickson JK,Nealson KH

更新日期：2007-11-01 00:00:00

abstract：:Bacteriophages against Streptococcus thermophilus are a growing problem in the Italian cheese industry. One possible control method involves replacing S. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. In this study, we evaluated lactose-positive pediococci for th...

journal_title：Applied and environmental microbiology

authors： Caldwell SL,McMahon DJ,Oberg CJ,Broadbent JR

更新日期：1996-03-01 00:00:00

abstract：:A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceed...

journal_title：Applied and environmental microbiology

authors： Lauff JJ,Steele DB,Coogan LA,Breitfeller JM

更新日期：1990-11-01 00:00:00

abstract：:Escherichia coli O157:H7 carried on plant surfaces, including alfalfa sprouts, has been implicated in food poisoning and outbreaks of disease in the United States. Adhesion to cell surfaces is a key component for bacterial establishment and colonization on many types of surfaces. Several E. coli O157:H7 surface protei...

journal_title：Applied and environmental microbiology

authors： Torres AG,Jeter C,Langley W,Matthysse AG

更新日期：2005-12-01 00:00:00

abstract：:Phages play a key role in the marine environment by regulating the transfer of energy between trophic levels and influencing global carbon and nutrient cycles. The diversity of marine phage communities remains difficult to characterize because of the lack of a signature gene common to all phages. Recent studies have d...

journal_title：Applied and environmental microbiology

authors： Goldsmith DB,Crosti G,Dwivedi B,McDaniel LD,Varsani A,Suttle CA,Weinbauer MG,Sandaa RA,Breitbart M

更新日期：2011-11-01 00:00:00

abstract：:Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...

journal_title：Applied and environmental microbiology

authors： Murinda SE,Roberts RF,Wilson RA

更新日期：1996-09-01 00:00:00

abstract：:The design of fully or partly defined media for mycoplasma cultivation involves the need to provide the essential lipids, cholesterol and long-chain fatty acids, in an assimilable and nontoxic form. This study introduces cyclodextrins (CDs) as carriers of these lipids, thus suggesting alternatives to serum or bovine s...

journal_title：Applied and environmental microbiology

authors： Greenberg-Ofrath N,Terespolosky Y,Kahane I,Bar R

更新日期：1993-02-01 00:00:00

abstract：:Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-alpha2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-density cultivat...

journal_title：Applied and environmental microbiology

authors： Sletta H,Tøndervik A,Hakvåg S,Aune TE,Nedal A,Aune R,Evensen G,Valla S,Ellingsen TE,Brautaset T

更新日期：2007-02-01 00:00:00

abstract：:A total of 52 strains of Lactobacillus acidophilus were examined for production of bacteriocins. A majority (63%) demonstrated inhibitory activity against all members of a four-species grouping of Lactobacillus leichmannii, Lactobacillus bulgaricus, Lactobacillus helveticus, and Lactobacillus lactis. Four L. acidophil...

journal_title：Applied and environmental microbiology

authors： Barefoot SF,Klaenhammer TR

更新日期：1983-06-01 00:00:00

abstract：:We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. col...

journal_title：Applied and environmental microbiology

authors： Salinger N,Kokona B,Fairman R,Okeke IN

更新日期：2009-01-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00

abstract：:Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated...

journal_title：Applied and environmental microbiology

authors： Paddock CD,Fournier PE,Sumner JW,Goddard J,Elshenawy Y,Metcalfe MG,Loftis AD,Varela-Stokes A

更新日期：2010-05-01 00:00:00

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:The human intestine harbors both lactate-producing and lactate-utilizing bacteria. Lactate is normally present at <3 mmol liter(-1) in stool samples from healthy adults, but concentrations up to 100 mmol liter(-1) have been reported in gut disorders such as ulcerative colitis. The effect of different initial pH values...

journal_title：Applied and environmental microbiology

authors： Belenguer A,Duncan SH,Holtrop G,Anderson SE,Lobley GE,Flint HJ

更新日期：2007-10-01 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

journal_title：Applied and environmental microbiology

authors： Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

更新日期：2005-11-01 00:00:00

abstract：:A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fift...

journal_title：Applied and environmental microbiology

authors： Terrón-González L,Martín-Cabello G,Ferrer M,Santero E

更新日期：2016-04-04 00:00:00

abstract：:Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

journal_title：Applied and environmental microbiology

authors： Bender J,Vatcharapijarn Y,Jeffries TW

更新日期：1985-03-01 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...

journal_title：Applied and environmental microbiology

authors： Booker AE,Hoyt DW,Meulia T,Eder E,Nicora CD,Purvine SO,Daly RA,Moore JD,Wunch K,Pfiffner SM,Lipton MS,Mouser PJ,Wrighton KC,Wilkins MJ

更新日期：2019-05-30 00:00:00

abstract：:Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

更新日期：1999-09-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：:The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

journal_title：Applied and environmental microbiology

authors： Gayán E,Cambré A,Michiels CW,Aertsen A

更新日期：2016-10-27 00:00:00

abstract：:During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previo...

journal_title：Applied and environmental microbiology

authors： Pomeroy LR,Sheldon JE,Sheldon WM

更新日期：1994-01-01 00:00:00

abstract：:An accurate most-probable-number enumeration method was developed for counting the number of Beggiatoa trichomes from various freshwater sediments. The medium consisted of extracted hay, diluted soil extract, 0.05% acetate, and 15 to 35 U of catalase per ml. The same enrichment medium, but without the acetate, was the...

journal_title：Applied and environmental microbiology

authors： Strohl WR,Larkin JM

更新日期：1978-11-01 00:00:00

abstract：:A novel xylanase (xylanase IV) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of Aeromonas caviae ME-1. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase IV molecular weight was 41,000. Xylanase IV catalyze...

journal_title：Applied and environmental microbiology

authors： Kubata BK,Takamizawa K,Kawai K,Suzuki T,Horitsu H

更新日期：1995-04-01 00:00:00

abstract：:Saccharomyces pastorianus strains are hybrids of Saccharomyces cerevisiae and Saccharomyces eubayanus that have been domesticated for centuries in lager beer brewing environments. As sequences and structures of S. pastorianus genomes are being resolved, molecular mechanisms and evolutionary origins of several industri...

journal_title：Applied and environmental microbiology

authors： Brouwers N,Brickwedde A,Gorter de Vries AR,van den Broek M,Weening SM,van den Eijnden L,Diderich JA,Bai FY,Pronk JT,Daran JG

更新日期：2019-10-30 00:00:00