Expanded Fermi solution for estimating the survival of ingested pathogenic and probiotic microbial cells and spores.

abstract：:A new virulent phage belonging to the Siphoviridae family and able to infect Lactococcus garvieae strains was isolated from compost soil. Phage GE1 has a prolate capsid (56 by 38 nm) and a long noncontractile tail (123 nm). It had a burst size of 139 and a latent period of 31 min. Its host range was limited to only tw...

journal_title：Applied and environmental microbiology

authors： Eraclio G,Tremblay DM,Lacelle-Côté A,Labrie SJ,Fortina MG,Moineau S

更新日期：2015-12-01 00:00:00

abstract：:Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

journal_title：Applied and environmental microbiology

authors： Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

更新日期：1999-11-01 00:00:00

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00

abstract：:There is much interest in the use of seed-applied bacteria for biocontrol and biofertilization, and several commercial products are available. However, many attempts to use this strategy fail because the seed-applied bacteria do not colonize the rhizosphere. Mechanisms of rhizosphere colonization may involve active ba...

journal_title：Applied and environmental microbiology

authors： Knox OG,Killham K,Artz RR,Mullins C,Wilson M

更新日期：2004-08-01 00:00:00

abstract：:Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were ...

journal_title：Applied and environmental microbiology

authors： Donegan K,Fieland V,Fowles N,Ganio L,Seidler R

更新日期：1992-04-01 00:00:00

abstract：:Sequence analysis of the five genes (gutRMCBA) downstream from the previously described sorbitol-6-phosphate dehydrogenase-encoding Lactobacillus casei gutF gene revealed that they constitute a sorbitol (glucitol) utilization operon. The gutRM genes encode putative regulators, while the gutCBA genes encode the EIIC, E...

journal_title：Applied and environmental microbiology

authors： Alcántara C,Sarmiento-Rubiano LA,Monedero V,Deutscher J,Pérez-Martínez G,Yebra MJ

更新日期：2008-09-01 00:00:00

abstract：:Lactobacillus bulgaricus LT4(0448) is a lysogenic strain from which a temperate bacteriophage can be induced by mitomycin C or UV irradiation. Lactobacillus lactis CNRZ 326 is an indicator strain for the temperate phage 0448, but this strain lyses only in the presence of Ca ions. A resistant culture developed secondar...

journal_title：Applied and environmental microbiology

authors： Cluzel PJ,Serio J,Accolas JP

更新日期：1987-08-01 00:00:00

abstract：:Wax-coated capsules containing selective ingredients (brilliant green and oxgall) were added at the time of inoculation of most-probable-number media (modified lactose broths). The inhibitory ingredients gradually diffused from the capsules into the nonselective media, imparting selectivity to the media. Concentration...

journal_title：Applied and environmental microbiology

authors： Lanz WW,Hartman PA

更新日期：1976-11-01 00:00:00

abstract：:Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are chara...

journal_title：Applied and environmental microbiology

authors： McMurdie PJ,Behrens SF,Holmes S,Spormann AM

更新日期：2007-04-01 00:00:00

abstract：:Most of the edible mushrooms cannot be cultivated or have low yield under industrial conditions, partially due to the lack of knowledge on how basidioma (fruiting body) development is regulated. From winter mushroom (Flammulina velutipes), one of the most popular industrially cultivated mushrooms, a transcription fact...

journal_title：Applied and environmental microbiology

authors： Wu T,Hu C,Xie B,Zhang L,Yan S,Wang W,Tao Y,Li S

更新日期：2019-11-27 00:00:00

abstract：:In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET prote...

journal_title：Applied and environmental microbiology

authors： Swingle B,Bao Z,Markel E,Chambers A,Cartinhour S

更新日期：2010-08-01 00:00:00

abstract：:The fungus Cladosporium sphaerospermum was isolated from a biofilter used for the removal of toluene from waste gases. This is the first report describing growth of a eukaryotic organism with toluene as the sole source of carbon and energy. The oxygen consumption rates, as well as the measured enzyme activities, of to...

journal_title：Applied and environmental microbiology

authors： Weber FJ,Hage KC,de Bont JA

更新日期：1995-10-01 00:00:00

abstract：:Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

journal_title：Applied and environmental microbiology

authors： Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

更新日期：1986-03-01 00:00:00

abstract：:Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all...

journal_title：Applied and environmental microbiology

authors： Haas JH,Moore LW,Ream W,Manulis S

更新日期：1995-08-01 00:00:00

abstract：:Phylogeny and the distribution of symbiotic bacteria in the mixed segment of the wood-eating termite Nasutitermes takasagoensis (Shiraki) were studied. Bacterial 16S rRNA genes (rDNA) were amplified from the mixed segment of the gut by PCR, and two kinds of sequences were identified. The phylogenetic tree was construc...

journal_title：Applied and environmental microbiology

authors： Tokuda G,Yamaoka I,Noda H

更新日期：2000-05-01 00:00:00

abstract：:The microbial assemblages of Lake Cisó and Lake Vilar (Banyoles, northeast Spain) were analyzed in space and time by microscopy and by performing PCR-denaturing gradient gel electrophoresis (DGGE) and sequence analysis of 16S rRNA gene fragments. Samples obtained from different water depths and at two different times ...

journal_title：Applied and environmental microbiology

authors： Casamayor EO,Schäfer H,Bañeras L,Pedrós-Alió C,Muyzer G

更新日期：2000-02-01 00:00:00

abstract：:We examined the bacterial communities of epilithic biofilms in 17 streams which represented a gradient ranging from relatively pristine streams to streams highly impacted by acid mine drainage (AMD). A combination of automated ribosomal intergenic spacer analysis with multivariate analysis and ordination provided a se...

journal_title：Applied and environmental microbiology

authors： Lear G,Niyogi D,Harding J,Dong Y,Lewis G

更新日期：2009-06-01 00:00:00

abstract：:The conversion of ochratoxin C to ochratoxin A was studied in rats after oral and intravenous administration. The concentration of ochratoxin A in the blood as a function of time was the same after oral administration of equivalent amounts of either ochratoxin C or ochratoxin A. The maximum ochratoxin A concentrations...

journal_title：Applied and environmental microbiology

authors： Fuchs R,Hult K,Peraica M,Radić B,Plestina R

更新日期：1984-07-01 00:00:00

abstract：:Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Caputo RA,Graham GS

更新日期：1981-06-01 00:00:00

abstract：:Fully grown broth cultures of various fast- and slow-growing rhizobia were deliberately diluted with various diluents before their aseptic incorporation into autoclaved peat in polypropylene bags (aseptic method) or mixed with the peat autoclaved in trays (tray method). In a factorial experiment with the aseptic metho...

journal_title：Applied and environmental microbiology

authors： Somasegaran P

更新日期：1985-08-01 00:00:00

abstract：:Bottlenecks in protein expression and secretion often limit the development of industrial processes. By manipulating chaperone and foldase levels, improvements in yeast secretion were found for a number of proteins. Recently, sustained endoplasmic reticulum stress, occurring due to recombinant protein production, was ...

journal_title：Applied and environmental microbiology

authors： Raimondi S,Zanni E,Talora C,Rossi M,Palleschi C,Uccelletti D

更新日期：2008-12-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:We analyzed the communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellogg Biological Station Long-Term Ecological Research site near Battle Creek, Michigan. The approach combined soil DNA extraction through a bead-beating method modified to increase recovery of fungal DNA, PCR ...

journal_title：Applied and environmental microbiology

authors： Lynch MD,Thorn RG

更新日期：2006-11-01 00:00:00

abstract：:Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA extraction methods (DN...

journal_title：Applied and environmental microbiology

authors： Jiang J,Alderisio KA,Singh A,Xiao L

更新日期：2005-03-01 00:00:00

abstract：:This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,Altermann E,Goh YJ,Tallon R,Sanozky-Dawes RB,Pfeiler EA,O'Flaherty S,Buck BL,Dobson A,Duong T,Miller MJ,Barrangou R,Klaenhammer TR

更新日期：2008-08-01 00:00:00

abstract：:Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH,...

journal_title：Applied and environmental microbiology

authors： Wilkins JC,Homer KA,Beighton D

更新日期：2001-08-01 00:00:00

abstract：:The use of two semisolid media (semisolid Rappaport and semisolid Rappaport-Vassiliadis) for the isolation of Salmonella spp. from coastal waters was compared with the use of conventional media. Two hundred and fifty six samples were studied; Salmonella spp. were detected in 83. The semisolid media were the most sensi...

journal_title：Applied and environmental microbiology

authors： Perales I,Audicana A

更新日期：1989-11-01 00:00:00

abstract：:The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved ...

journal_title：Applied and environmental microbiology

authors： Bachmann H,Santos F,Kleerebezem M,van Hylckama Vlieg JE

更新日期：2007-07-01 00:00:00

abstract：:Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

journal_title：Applied and environmental microbiology

authors： Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

更新日期：1998-08-01 00:00:00

abstract：:Bacteriophage resistance mechanisms which are derived from a bacteriophage genome are termed Per (phage-encoded resistance). When present in trans in Lactococcus lactis NCK203, Per50, the cloned origin of replication from phage phi50, interferes with phi50 replication. The per50 fragment was found to afford negligible...

journal_title：Applied and environmental microbiology

authors： O'sullivan DJ,Hill C,Klaenhammer TR

更新日期：1993-08-01 00:00:00