Recombineering using RecTE from Pseudomonas syringae.

abstract：:The initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. Characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. Usi...

journal_title：Applied and environmental microbiology

authors： Diaz PI,Chalmers NI,Rickard AH,Kong C,Milburn CL,Palmer RJ Jr,Kolenbrander PE

更新日期：2006-04-01 00:00:00

abstract：:Fusarium verticillioides (Gibberella fujikuroi mating population A [MP-A]) is a widespread pathogen on maize and is well-known for producing fumonisins, mycotoxins that cause severe disease in animals and humans. The species is a member of the Gibberella fujikuroi species complex, which consists of at least 11 differe...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Hedden P,Tudzynski B

更新日期：2008-12-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and n...

journal_title：Applied and environmental microbiology

authors： Nassar A,Darrasse A,Lemattre M,Kotoujansky A,Dervin C,Vedel R,Bertheau Y

更新日期：1996-07-01 00:00:00

abstract：:White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely ...

journal_title：Applied and environmental microbiology

authors： Shuey MM,Drees KP,Lindner DL,Keim P,Foster JT

更新日期：2014-03-01 00:00:00

abstract：:Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...

journal_title：Applied and environmental microbiology

authors： Chesson A,Stewart CS,Wallace RJ

更新日期：1982-09-01 00:00:00

abstract：:Anaerobic ammonium-oxidizing (anammox) bacteria have been recognized as an important sink for fixed nitrogen and are detected in many natural environments. However, their presence in terrestrial ecosystems has long been overlooked, and their contribution to the nitrogen cycling in natural and agricultural soils is cur...

journal_title：Applied and environmental microbiology

authors： Hu BL,Rush D,van der Biezen E,Zheng P,van Mullekom M,Schouten S,Sinninghe Damsté JS,Smolders AJ,Jetten MS,Kartal B

更新日期：2011-02-01 00:00:00

abstract：:Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

journal_title：Applied and environmental microbiology

authors： Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

更新日期：2019-07-01 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum str...

journal_title：Applied and environmental microbiology

authors： Bakan B,Giraud-Delville C,Pinson L,Richard-Molard D,Fournier E,Brygoo Y

更新日期：2002-11-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:The induction of the inducible lysyl-tRNA synthetase, LysU, and the inducible lysine and arginine decarboxylases of Escherichia coli K-12 grown in AC broth to a pH of 5.5 or less is temperature dependent, being distinctly lower at 24 than at 37 degrees C. This induction does not appear to be under HtpR control. ...

journal_title：Applied and environmental microbiology

authors： Hassani M,Pincus DH,Bennett GN,Hirshfield IN

更新日期：1992-08-01 00:00:00

abstract：:Comparative genomic hybridizations have been used to examine genetic relationships among bacteria. The microarrays used in these experiments may have open reading frames from one or more reference strains (whole-genome microarrays), or they may be composed of random DNA fragments from a large number of strains (mixed-...

journal_title：Applied and environmental microbiology

authors： Wan Y,Broschat SL,Call DR

更新日期：2007-03-01 00:00:00

abstract：:Many but not all ammonia-oxidizing bacteria (AOB) produce urease (urea amidohydrolase, EC 3.5.1.5) and are capable of using urea for chemolithotrophic growth. We sequenced the urease operons from two AOB, the beta-proteobacterium Nitrosospira sp. strain NpAV and the gamma-proteobacterium Nitrosococcus oceani. In both ...

journal_title：Applied and environmental microbiology

authors： Koper TE,El-Sheikh AF,Norton JM,Klotz MG

更新日期：2004-04-01 00:00:00

abstract：:Spontaneous vegetable fermentations, with their rich flavors and postulated health benefits, are regaining popularity. However, their microbiology is still poorly understood, therefore raising concerns about food safety. In addition, such spontaneous fermentations form interesting cases of man-made microbial ecosystem...

journal_title：Applied and environmental microbiology

authors： Wuyts S,Van Beeck W,Oerlemans EFM,Wittouck S,Claes IJJ,De Boeck I,Weckx S,Lievens B,De Vuyst L,Lebeer S

更新日期：2018-05-31 00:00:00

abstract：:Botulinum neurotoxin (BoNT), produced by neurotoxigenic clostridia, is the most potent biological toxin known and the causative agent of the paralytic disease botulism. The nutritional, environmental, and genetic regulation of BoNT synthesis, activation, stability, and toxin complex (TC) formation is not well studied....

journal_title：Applied and environmental microbiology

authors： Fredrick CM,Lin G,Johnson EA

更新日期：2017-06-16 00:00:00

abstract：:Air drying of raw sludge caused inactivation of indigenous viruses. A gradual loss of infectivity occurred with the loss of water until the solids content reached about 80%. A more rapid decline of viral infectivity occurred with further dewatering. ...

journal_title：Applied and environmental microbiology

authors： Brashear DA,Ward RL

更新日期：1983-06-01 00:00:00

abstract：UNLABELLED:A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalyt...

journal_title：Applied and environmental microbiology

authors： Horiuchi A,Aslam M,Kanai T,Atomi H

更新日期：2016-05-31 00:00:00

abstract：:The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

journal_title：Applied and environmental microbiology

authors： Zappe H,Jones WA,Jones DT,Woods DR

更新日期：1988-05-01 00:00:00

abstract：:rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...

journal_title：Applied and environmental microbiology

authors： Parker MA

更新日期：2002-04-01 00:00:00

abstract：:pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Salyers AA

更新日期：1985-10-01 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by...

journal_title：Applied and environmental microbiology

authors： Klijn N,Weerkamp AH,de Vos WM

更新日期：1995-07-01 00:00:00

abstract：:The importance of viruses in marine microbial ecology has been established over the past decade. Specifically, viruses influence bacterial abundance and community composition through lysis and alter bacterial genetic diversity through transduction and lysogenic conversion. By contrast, the abundance and distribution o...

journal_title：Applied and environmental microbiology

authors： Williamson KE,Radosevich M,Wommack KE

更新日期：2005-06-01 00:00:00

abstract：:Eighteen of 1,200 colonies of Escherichia coli isolated from "keebe," hamburger, or sausage produced heat-labile enterotoxin. None of them produced heat-stable enterotoxin. The characteristics of 9 of the 18 strains are presented. ...

journal_title：Applied and environmental microbiology

authors： Reis MH,Vasconcelos JC,Trabulsi LR

更新日期：1980-01-01 00:00:00

abstract：:Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated wi...

journal_title：Applied and environmental microbiology

authors： Kubota H,Mitani A,Niwano Y,Takeuchi K,Tanaka A,Yamaguchi N,Kawamura Y,Hitomi J

更新日期：2012-05-01 00:00:00

abstract：:It has been hypothesized that the environment can influence the composition of the nasal microbiota. However, the direct influence of pig farming on the anterior and posterior nasal microbiota is unknown. Using a cross-sectional design, pig farms (n = 28) were visited in 2014 to 2015, and nasal swabs from 43 pig farme...

journal_title：Applied and environmental microbiology

authors： Kraemer JG,Ramette A,Aebi S,Oppliger A,Hilty M

更新日期：2018-03-01 00:00:00

abstract：:Penicillium expansum isolated from meat and apples produced both patulin and citrinin. Toxin identity was confirmed by spectroscopic and physical methods. The mean lethal dose in chicken embryos was determined for toxins administered both singly and in various ratios. Data from simultaneous administration of mycotoxin...

journal_title：Applied and environmental microbiology

authors： Ciegler A,Vesonder RF,Jackson LK

更新日期：1977-04-01 00:00:00